We narrowed to 468 results for: SEC

1. Introducing Addgene's New Search - Find Plasmids More Easily For Your Research!

   Type

   Blog Post

   Published

   Dec. 3, 2019, 11:38 a.m.

   ... a convenient most requested filter. Applying a second filter, this one from the ‘Requests’ category, ...

2. CRISPR 101: Making a Knock-In Cell Line

   Type

   Blog Post

   Published

   Dec. 22, 2022, 2:15 p.m.

   ...possible to the knock-in location. HDR depends on resection at the break site to form ssDNA and uses the ends...

3. Plasmids 101: Colony PCR

   Type

   Blog Post

   Published

   May 12, 2016, 2:30 p.m.

   ...plasmid backbone. Backbone-specific primers: A second option is to design backbone-specific primers. ...

4. CRISPR Antimicrobials

   Type

   Blog Post

   Published

   May 3, 2016, 2:30 p.m.

   ...targeting 1) the given resistance gene and 2) a second lytic phage. Bacteria that receive this phage are...

5. Tips for Screening with Yeast Two Hybrid Systems

   Type

   Blog Post

   Published

   Oct. 22, 2015, 2:30 p.m.

   ...single-pass two-hybrid screening (6). See the Tips section for more on how to increase your rate of interactions...

6. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...s possible to link between various documents. A second example is the integration between RSpace and Slack...

7. CRISPR-mediated Plant Base Editors

   Type

   Blog Post

   Published

   Jan. 3, 2019, 1:35 p.m.

   ...uracil glycosylase inhibitor (UGI) minimizes the second outcome and increases the generation of the desired...

8. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

   Type

   Blog Post

   Published

   Nov. 1, 2018, 12:35 p.m.

   ... cells that have removed the cassette through a second recombination event between the two flanking repeats...

9. Finding nucleic acids with SHERLOCK and DETECTR

   Type

   Blog Post

   Published

   April 16, 2020, 1 p.m.

   ...subsequently degraded at a rate of ~1,250 cuts per second. A quantifiable fluorescent signal designates the...

10. Plasmids 101: Plasmid Incompatibility

    Type

    Blog Post

    Published

    March 31, 2020, 1:15 p.m.

    ...repressed and replication is downregulated. In the second model, termed the “handcuff” model, iterons of ...

11. New CRISPR Tools: Cas7-11 and PASTE

    Type

    Blog Post

    Published

    Feb. 17, 2022, 2 p.m.

    ...The first generation of CRISPR could cut DNA, the second generation could do base editing, and with the ...

12. Hot Plasmids - August 2020

    Type

    Blog Post

    Published

    Aug. 18, 2020, 1:15 p.m.

    ...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...

13. CRISPR 101: Any Base Transversion Editors

    Type

    Blog Post

    Published

    April 1, 2025, 1:15 p.m.

    .... ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE C → G Base...

14. Beyond the Blood Brain Barrier: PHP.B Enters the Central Nervous System Via the LY6A Receptor

    Type

    Blog Post

    Published

    July 23, 2019, 12:46 p.m.

    ...PHP.B transduction as their C57BL/6J parent. The second generation (F2) of outbred mice also showed a Mendelian...

15. New Tools Enable CRISPRa for Neuroscience Applications

    Type

    Blog Post

    Published

    July 2, 2019, 12:51 p.m.

    ... Thus, CRISPRa represents a powerful method to dissect the individual and shared contributions of transcript...

16. Viral Production at Addgene

    Type

    Blog Post

    Published

    Oct. 24, 2023, 1:15 p.m.

    ...page for the corresponding catalog item (see maps section for images).     Figure 2: AAV Pro cells...

17. Viral Vectors 101: Preparing Pooled Libraries

    Type

    Blog Post

    Published

    July 13, 2023, 1:15 p.m.

    ...associated with the library and reading their methods sections to see what worked.  Ready, optimize, transduce...

18. Neuronal labeling with Spaghetti Monster

    Type

    Blog Post

    Published

    Aug. 14, 2018, 12:42 p.m.

    ...channel. In multi-color imaging experiments, the second color is usually provided by red fluorescent proteins...

19. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...duplicated during lentiviral integration, producing a second copy of the sgRNAs. Image reused from Walton et...

20. Antibodies 101: Reading a Flow Plot

    Type

    Blog Post

    Published

    Feb. 6, 2024, 3:13 p.m.

    ...because we usually assume (0,0) to be at the intersection of X,Y. For flow plots, however, that is generally...