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We narrowed to 478 results for: SEC

Showing: 401 - 420 of 478 results
  1. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...mAmetrine1.2). However, for most FPs it is a few seconds to a few minutes. Photostability can be affected...
  2. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...possible to the knock-in location. HDR depends on resection at the break site to form ssDNA and uses the ends...
  3. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ... such as the chicken beta-actin promoter of the pHLsec vector. In this scenario, IVA can be employed by...
  4. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...plasmid backbone. Backbone-specific primers: A second option is to design backbone-specific primers. ...
  5. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...targeting 1) the given resistance gene and 2) a second lytic phage. Bacteria that receive this phage are...
  6. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...duplicated during lentiviral integration, producing a second copy of the sgRNAs. Image reused from Walton et...
  7. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...because we usually assume (0,0) to be at the intersection of X,Y. For flow plots, however, that is generally...
  8. Viral Production at Addgene

    Type
    Blog Post
    Published
    Oct. 24, 2023, 1:15 p.m.
    ...page for the corresponding catalog item (see maps section for images).     Figure 2: AAV Pro cells...
  9. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...The first generation of CRISPR could cut DNA, the second generation could do base editing, and with the ...
  10. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...exported to the periplasm of bacteria where it can be secreted into the supernatant. Four plasmids containing...
Showing: 401 - 420 of 478 results