We narrowed to 524 results for: SHE

1. Viral Vectors 101: Viruses as Biological Tools

   Type

   Blog Post

   Published

   March 21, 2023, 1:15 p.m.

   ...specific. Once inside the host cell, the virus unleashes its cargo – its own genome.   Fig. 1:...

2. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

   Type

   Blog Post

   Published

   Nov. 19, 2019, 2:08 p.m.

   ...of endogenous signaling activity in cells.  Let’s shed some light on the key components of OptoNBs, how...

3. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...In January 2016 we first published a blog post titled: Which Cas9 Do I Choose for My CRISPR Experiment...

4. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

   Type

   Blog Post

   Published

   July 28, 2017, 1:30 p.m.

   ...chromosome your transgene has integrated. This can be accomplished with a few matings to balancer chromosome lines...

5. Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection

   Type

   Blog Post

   Published

   May 11, 2017, 2:30 p.m.

   ...gene of poorly classified function and a well established cellular signaling gene, thus providing a starting...

6. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ...Antibodies 101: Introduction to Antibodies for a refresher). There are five antibody isotypes that each have...

7. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ... the example of our Antibody Guide, which was published alongside our new antibody services in the spring...

8. Hot Plasmids - February 2022

   Type

   Blog Post

   Published

   Feb. 22, 2022, 2:15 p.m.

   ...Stasevich and Hiroshi Kimura’s labs have recently published a set of anti-FLAG frankenbodies, a flexible, ...

9. Antibodies 101: Buffers, Storage, and Conjugates

   Type

   Blog Post

   Published

   Feb. 1, 2022, 2:15 p.m.

   ... the buffer, which will be noted on the product sheet. Thankfully, sodium azide can easily be removed ...

10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type

    Blog Post

    Published

    May 26, 2020, 1:15 p.m.

    ...posts Find more about fluorescent proteins Get a refresher on CRISPR with our CRISPR 101 blog posts Resources...

11. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

    Type

    Blog Post

    Published

    May 19, 2020, 1:15 p.m.

    ... from this collection in your experiments and published your data, please fill out this form and we’ll...

12. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...survive, but thrive, in the face of some of Earth’s harshest environments: corrosive pH ranges, crushing pressures...

13. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...lentiviral) or pUMVC (MuLV retroviral). Fun fact: published in 2003, this plasmid predates Addgene’s founding...

14. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

    Type

    Blog Post

    Published

    July 28, 2015, 2:30 p.m.

    ...attention on transcription factors, starting with an established method called ChIP-seq. ChIP-seq relies on antibodies...

15. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang’s group did just that as part...

16. How to Make Friends and Meet People at a Scientific Conference

    Type

    Blog Post

    Published

    Aug. 7, 2014, 1:58 p.m.

    ...Congratulate your connections when they get a new paper published. Stay in touch enough to plan to meet up at the...

17. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type

    Blog Post

    Published

    Dec. 10, 2013, 3:13 p.m.

    ...moment you are designing a TALEN on paper to a finished plasmid. With our protocol, it is feasible to ...

18. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...conjugated antibodies that are ready to use off the shelf. So why are we talking about this? Even though there...

19. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type

    Blog Post

    Published

    Sept. 4, 2018, 12:53 p.m.

    ...PubMed PMID: 29553576. 2. Kittleson, Joshua T., Sherine Cheung, and JChristopher Anderson. "Rapid optimization...

20. Viral Vectors 101: An Introduction to AAV

    Type

    Blog Post

    Published

    May 30, 2023, 1:15 p.m.

    ...Serotype refers to the type of capsid (outer protein shell) used to package an rAAV vector; these capsids allow...