Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 609 results for: cas

Showing: 401 - 420 of 609 results

  1. Fluorescent Proteins 101: Aptamer Fluorophores

    Type
    Blog Post
    Published
    April 11, 2017, 2:30 p.m.
    ...relies on dimerization for activation, only in this case ligand binding would bring the relevant ends together...RNA-based scaffolds to contain and stabilize Broccoli cassettes, thereby extending their use into mammalian cells...

  2. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...research being published on our own topic. Only on occasion do we then have time to read about research from...video abstracts in science journals: An overview and case study." Journal of Librarianship and Scholarly Communication...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...hydrogen bonds holding the DNA together, it recruits helicases to continue unwinding the strands. This creates...present in the chromosomal DNA ensure that the helicases are released from the DNA once the replication...

  4. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ...initially isolated. Regrettably this is not usually the case if you obtain a cell line from a colleague. Time... been directly isolated from patients. In these cases, a test known as Short Tandem Repeat (STR) profiling...

  5. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...density within a reasonable time-frame. For these cases, some strains carry an additional measure of control... plasmid contains a chloramphenicol resistance cassette for positive selection and a p15A origin of replication...

  6. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...approach is reliable, but it’s overkill in many cases. Often, you may not need to know the exact quantity...Although this assumption is likely true in many cases, you should validate it for your particular setup...

  7. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...is that my suggestions can help you make the best case for your candidacy. A major reason that a lot of...encouraged me to publish this manual and earn some cash for my expensive habits of running shoes and single...

  8. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...approaches to record keeping. Top priorities in most cases include IP protection, publications and funding....site licenses might be available as well. In most cases this applies to universities. How are you currently...

  9. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...any additional work you might need to do. In most cases, I would recommend the second option for the experiment... gave me time to listen to some of my favorite podcasts. JUST GET STARTED - One Harvard Business Review...

  10. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...to the occasion Don’t just plan to reuse a prepared talk unless it perfectly fits the occasion.  I speak...

  11. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ... film or with specific imaging devices. In most cases, the primary antibody, which binds the protein of...inconsistent levels of the loading control. In these cases, the western should be repeated.  Finally, while...

  12. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ... will increase the power of the results. In this case, mouse samples from positive (+), negative (-) and...antibodies will be highly specific, but in some cases they may cross-react and bind to more than their...

  13. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...to knock-down expression of your target or CRISPR/Cas9 to create mutations that essentially eliminate expression...gene for your protein has been mutated using CRISPR/Cas9. You check the mutation and it causes a large deletion...

  14. Antibodies 101: Antibody Engineering and Directed Evolution

    Type
    Blog Post
    Published
    Aug. 5, 2025, 1:15 p.m.
    ...-fidelity polymerase to ensure accuracy. In this case, you would want a low-fidelity polymerase, like ...different chimeras might function better. In that case, gene shuffling might be a good option. In gene ...

  15. Deep Dive: Statistical Tests (Comparisons)

    Type
    Blog Post
    Published
    March 7, 2023, 2:15 p.m.
    ...designations all refer to the same test. On some occasions, there is an experimental group being compared... being between control vs. experimental. In this case, save yourself from family-wise error and just perform...

  17. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...antibodies together in the same color panel. In this case, make sure to compare the excitation/emission spectra... so it's better to use a brighter fluorophore in case the CD45 expression turns out to be weak. Table ...

  18. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...been infected with a microbe, to trigger immune cascades that clear these infections. Anything that generates...immune response and initiates different immune cascades. In the research setting, antibodies of different...

  19. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ... double peak even with a single product. In that case, you’ll want to validate your primer/probe through...Higgins, 2000; Ref, Zhu, 2002), though there are cases in which it is an appropriate choice (Panina, 2018...

  20. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ... This makes it possible to use a polycistronic cassette that expresses multiple reprogramming factors ...minicircle expressing a 2A peptide polycistronic cassette comprised of OCT4, SOX2, LIN28, NANOG, and a GFP...
Showing: 401 - 420 of 609 results