We narrowed to 695 results for: prot

...are a number of drawbacks to this approach: TET proteins are not demethylases; they are dioxygenases that...rare DNA bases that are recognized by distinct proteins and result in unclear transcriptional consequences..., Charlton et al. 2020). Most importantly, TET proteins affect transcription independent of their enzymatic...may seem counterintuitive: how can a DNA-binding protein with no enzymatic activity cause demethylation?...

...potential to bind many different sites on the target protein they tend to have a strong signal and to work in...mouse derived primary antibody to stain a target protein in mouse tissues, causes a high degree of background...sample. There are species-on-species staining protocols that use antibody fragments to block the endogenous...Biological systems tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex...

...once. Fibronectin is another membrane-interacting protein that can be used to enhance transduction efficiency...Alternatively, if the virus encodes a fluorescent protein, the packaging cells could be examined with a microscope... While there may be some low-level fluorescent protein or resistance marker expression following transfection...Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

...drawbacks of different methods (for comprehensive protocols for all of the methods discussed here refer to...and are usually based on the presence of a viral protein, such as p24, or viral nucleic acid. Functional...staining. Check Out Our In-Depth Fluoresence Titering Protocol Another popular approach utilizes the antibiotic...pseudotyped HIV-1-based lentiviral vectors."Nature protocols 4.4 (2009): 495-505. PubMed PMID: 19300443. 2....

...remodeling. Chromatin is a complex of DNA and histone proteins that compacts cellular DNA into chromosomes and...localise the construct specifically to the histone protein of interest (in the figure above, H3 at K9 or K27...Lab plasmids at Addgene Browse More Fluorescent Protein Biosensors Read Our Tips for Using FRET in Your...Experiments Post Read Other Blog Posts about Fluorescent Proteins  ...

...details on this procedure check out our PCR protocol page, protocol video and reference on how to design PCR...Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol: How to Design a primer  ...

...thinking of an amazing project. You’ve written your protocols, designed your experiments, and prepared your ...purifications, or ligations. The general Gateway cloning protocol is fairly flexible and adaptable to cloning different... they will come with comprehensive and tested protocols, as well as premixed and ready-to-use reagents...101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find vector backbones Read...

...boundaries defined by anti-CRISPR proteins or running into other proteins naturally bound to the DNA. Cas3...Type I-E Cascades contains all five of these Cas proteins, while type I-C Cascades do not contain Cas6. ...Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems INTEGRATE: Bacterial...

...to tag all 22,000 protein coding genes in the human genome with fluorescent proteins. For each gene, we...guide sequence to every possible 23bp sequence (protospacer + PAM) in the target region after HDR. If the...outwards so that the DNA sequence is altered but the protein sequence they encode is not. After each mutation...

...previous work, Sakuma et al. describe a detailed protocol for MMEJ-mediated knock-in of a GFP-Puro cassette...much shorter sequence homologies. Abbreviated protocol for PITCh Step 1: Generate microhomologies in ...TALENs and CRISPR-Cas9 with the PITCh systems.” Nat Protoc. 11(1): 118-33. PMID: 26678082. Find the plasmids...Scalable Genome-Editing Based Approach for Mapping Multiprotein Complexes in Human Cells.” Cell Rep. 13: 621...

...construct on the same piece of DNA. We do a lot of protein-protein interaction work and we’ve gotten some tools...contains sequences for several different fluorescent proteins. Visualizing fluorescence can be challenging in...chlorophyll, so we looked at newer fluorescent proteins that get around that problem. MoClo is really ...

...resources: plasmid, pooled library, blog posts, protocols, and more. Selecting crispr in Guides will return...collection for an antibody against PARIS, a zinc finger protein involved in neurodegeneration in Parkinson’s disease...lentiviral prep needed Tags: Identify a specific marker protein Use: Similar to “Vector Type” you can filter by...about the subject, link to related guides and protocols, and provide easy to sort tables of hand-picked...

... tries to “optimize” a particular standard lab protocol. For example, someone may attempt super quick ...quick incubation times in a Western Blot protocol and then blame the antibody when no signals are obtained...so many things which can go wrong in a lengthy protocol. And customers may tell all sorts of details about...reviewing customer data sets, troubleshooting protocols, analyzing information, and solving customer problems...

...) the sequence is located just upstream of a Protospacer Adjacent Motif (PAM sequence). The 3-5 nucleotide...the CRISPR toolbox The isolation of novel CRISPR proteins has and will continue to dramatically increase...CRISPR applications. The first non-Cas9 CRISPR protein adapted for genome engineering was Cas12a (formerly...edit. Some Cas13 enzymes require a single base protospacer flanking sequence (PFS) adjacent to the target...

...producing vectors from plasmids — including the protocols we use in our lab — being able to order high quality...biosensors, optogenetic tools, and chemogenetic tools: protein tools that are activated, respectively, by substrates...educational resources, from eBooks to guides to protocols, which was why I was so excited to delve into ...Using Pooled Lentiviral CRISPR Libraries. Current Protocols in Molecular Biology, 115, 31.5.1-31.5.13. https...

...group box 1 protein. Normally localized to the nucleus, if the cell dies from injury, this protein leaks out... asbestos. However, as the U.S. Environmental Protection Agency has noted, there is no safe level of asbestos...thus ensuring that future generations will be protected from this dangerous material. This post was contributed...

...plasmid that constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected...your cells. Regardless, the amount of reporter protein activity correlates to both the amount of DNA transfected...cells and the ability of the cells to express the protein.   In analysis of the result in Figure 1, an internal...were transfected successfully and expressing the protein. For example, fluorescence microscopy images resulting...

...overhang. This will serve as both a substrate for proteins required for strand invasion and a primer for ...preferred for large insertions such as fluorescent proteins or selection cassettes. This class of template...the CRISPR-Cas9 system. Ran FA, et al. Nature Protocols Nov;8(11):2281-308; (2013). PubMed. A Mouse ...alleles using long ssDNA donors and CRISPR ribonucleoproteins. Quadros RM, et al. Genome Biol. 18(1); (...

...hybridization for detection of gene copy number changes." Protocols for Nucleic Acid Analysis by Nonradioactive Probes...engineering transgenic constructs and mice." Current Protocols in Cell Biology (2009): 19-10. PubMed PMID: 19283728...fourth-generation DNA sequencing technology." Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID...by PCR Resources on Addgene.org Check out Our Protocol Pages Find Molecular Biology Tools Analyze Your...

...of indels and thus frameshifts that disrupt the protein-coding capacity of a locus. When using S. pyogenes...code for amino acids near the N’ terminus of the protein, in order to mitigate the ability of the cell to...for amino acids close to the C’ terminus of the protein, to maximize the chances of creating a non-functional...nucleotides, restricting gRNAs to 5 – 65% of the protein coding region will still result in many dozens ...