We narrowed to 842 results for: Eras

...(2023) Nanobodies: A Review of Generation, Diagnostics and Therapeutics. Int J Mol Sci 24:5994. https:...The UN General Assembly has declared 2024 the International Year of Camelids. The declaration is intended...into the clinic as well. The first sdAb based therapeutic was approved in 2018, with many others in clinical...clinical trials (Jin, et al. 2023). In addition to therapeutics, there is a lot of excitement around using sdAbs... synthetic single-domain antibody libraries to generate new sdAbs. There has also been some interesting... (A) Overview of genetic modifications made to generate the nanomouse. (VHH = variable domains of heavy...their impact grow as new sdAbs become easier to generate and more widely adopted. So thank you to the camelids...

... due to the interaction with quizzes and discussion on that week’s topic by the moderators. The assignments...scientists. Over the course of the class, the moderator discussed individual sections of a paper, the ...laborious, but helped build my understanding of how to generate a manuscript. At the end of the online course,...understanding of how to apply it. The personal interaction of critiquing others’ manuscripts as well as ...the writer works.  4. Practice writing everyday Overall, the most important instruction the classes taught.... Over time that portion of the paper will be generated. After you complete a publication quality figure...figure. Writing a scientific manuscript is like generating a quilt with your lab mates, you add the individual...

...Addgene blog Important Considerations When Using AAVs Three Key Considerations For Precise Neuronal Targeting... for sharing on the site. The entry form has 5 general question types and it takes approximately 15 minutes...experiment, read our blog post on important considerations when using AAVs. Experimental results: These...virus? If you were using a genetic tool, did it operate as expected? If your experiment did not work, we...Frontiers in Neuroanatomy and summarized some considerations for precise neuronal targeting using AAV on...submission of an experimental image result that you generated with a caption. For microscopy images, please ...microscope used, and objective used. If you did not generate the image yourself and it is under copyright, ...

...high efficiency CRISPR-based approaches for the generation of knockins via homologous recombination with...approach is most well suited, since it routinely generates knockin efficiencies of 1-40% with G418-selected... CRISPR-based approach. Second, the AAV preps generally used for gene editing are low titre, and are therefore... most current CRISPR-based approaches for the generation of knockins, it is not always ideal. Because ...advantages of the AAV and CRISPR-based approaches, several groups have recently worked to enhance the efficiency...AAV and CRISPR-based approaches is described in several recent reviews (Howes and Schofield, 2015; Moser...PMC3010411. 2. Kohli, Manu, et al. "Facile methods for generating human somatic cell gene knockouts using recombinant...

...kits with greatly reduced costs per plasmid. We generally create kits to allow you to easily use a set of... by making it easy for a researcher to quickly generate N- and C-terminal epitope tag fusions to a protein...protein of interest. The plasmids used to generate these fusions all use the same restriction enzymes for...provided a solution for this problem by developing a general translation initiation sequence known as the Species-Independent...locus-specific libraries compatible with Illumina next generation sequencing in order to study evolutionary dynamics...These bridging primers are amplified and used to generate Illumina sequencing libraries using a 2-step PCR-based...open source platform and the libraries can be generated in a cost-effective manner with minimal bias. ...

...Standard AAV vectors are generated with a producer cell line like the widely used 293T. During vector...Casey Maguire and colleagues from Massachusetts General Hospital and Harvard Medical School took a closer... antibodies. This is particularly useful for therapeutic applications in vivo where endogenous anti-AAV...anti-AAV antibodies often neutralize the therapeutic efficacy of AAV vectors. How are exosome AAVs produced...exosomes or stabilize their structure or their interaction with AAVs (Schiller et al., 2018). Exosomal vesicles...interest special contexts, such as the delivery of therapeutic proteins to the brain which requires crossing...cells using exosome-associated AAV." Molecular Therapy 25.2 (2017): 379-391. PubMed PMID: 28082074. PubMed...

...and easily generate zebrafish transgenic lines to study neurobiology and neuorodgenerative diseases. The...Gateway-Compatible Toolbox is based on the original Tol2kit generated by the Chi-Bin Chien lab (Kwan et al., 2007) and... specific neuronal cell types involved in neurodegenerative diseases, the Cole Lab selected promoters ...The impact of KLF4 N-terminal variants on iPSC generation Deriving iPSCs (induced pluripotent stem cells...reprogramming somatic cells. A common method for generating iPSCs to express the transcription factors from...Polycistronic cassettes that contained KLF4s had overall diminished KLF4 expression levels; this altered...mScarlet. Bindels et al used a combination of deliberate and random mutagenesis to isolate the best RFP...

...(tracrRNA) which interacts with the Cas9 protein. These RNA molecules are vulnerable to attack by both...effectively serving their function of guiding Cas9 to generate a targeted break. These advances in gRNA technology...foreign RNA. To protect the RNA from degradation, several simple methods have been employed which modify ...modification is best? Studies suggest coupling several of them together, for example phosphorothioates...more efficient on a budget is to simply swap out several of the ribonucleotides for deoxynucleotides. A ...A partially DNA gRNA is surprisingly well-tolerated by the Cas9 protein and can increase on-target efficiency...photocleavable gRNAs. Photocleavable guides are generated by introduction of a single photocleavable 2-nitrobenzyl...

...neurons and astrocytes By Mike Lacy Cell-cell interactions underlie many kinds of tissue function, but ...To better understand how astrocytes and neurons interact, the labs of Baljit Khakh and colleagues mapped... The team expressed extracellularly-anchored horseradish peroxidase (HRP) in striatal astrocytes and neurons...studies of the molecular bases of astrocyte-neuron interactions. Plus, the tools used here (also available as...molecular foundations of their multicellular interactions. Neuron, 113(16), 2599–2620.e7. https://doi....peptide that caused nucleolar disruption into a general "killswitch" for other biomolecular condensates...this perturbation interrupted the functions of several endogenous or pathological condensates, including...

...Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region ...protein, the repressor protein can bind to the operator. The bound repressor then prevents transcription... repressor (TetR) can bind to the tetracycline operator sequences (TetO), preventing transcription. In...have been engineered and improved. Takara’s “2nd generation Tet-Off vectors (Tet-Advanced)” are the most ...VP16. cTA binds to promoters containing putative operator sequences (CuO) (linked in groups of 6), allowing...the same manner, with LexA binding to the lexA operator (lexAop). Fusing the LexA DNA-binding domain (...refined the Q system and introduced the second generation QF2 activators. These activators solve the problem...