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We narrowed to 729 results for: NSI

Showing: 211 - 220 of 729 results

  1. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...do you make the most of this new relationship? Consider adding formality and active goal setting to your...major advantage of working with a mentor.The responsibility of arranging meetings and setting the direction...meetings and discuss these in person. See the extensive resource list available from this post. Use an...items and make progress. To illustrate this point, consider that I try to exercise every morning, but the ...

  2. Creating accessible biology activities in schools with BioBits

    Type
    Blog Post
    Published
    Aug. 2, 2018, 12:56 p.m.
    ...lab knows how expensive tiny amounts of reagents are, how complicated (and again, expensive) the different... college level, perhaps consider using some elements of BioBits to transition your undergraduate students...

  3. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...of the University of British Columbia. When transitioning from an academic science career path to a non-academic...applying for the position?   Here are a few things to consider when you're preparing your resume for a non-academic...postings and look at the jobs you are interested in. Consider listing the skills you already have, and the skills...Originally on track for a research career, she transitioned into a communications role instead after she...

  4. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

    Type
    Blog Post
    Published
    Aug. 28, 2014, 3:34 p.m.
    ...the plasmids we distribute. This method is time-intensive, so we recommend a variety of ways to screen and...10kb, with the 3.0kb fragment having increased intensity to serve as a reference band. The uncut DNA (lane...accurately visualize the full size of your construct. Consider buffer and temperature compatibility when digesting...methylation issues. Enzymes like XbaI and ClaI are sensitive to methylation and their activity may be blocked...

  5. Fluorescent Proteins 101: Luciferase

    Type
    Blog Post
    Published
    June 24, 2014, 3:59 p.m.
    ...ideally would have low background signal, high sensitivity and of course be quick, accurate and safe. In...the chemical reaction compared to using a high-intensity laser to rapidly excite GFP. As a result of the... lower background levels and improved signal sensitivity since photons are simply measured – they are ...Not Required Signal Strength Lower Higher Sensitivity Higher Lower Background Lower Higher Post-translational...

  6. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ...Institute were instrumental in helping us establish consistent, high-quality protocols for lentiviral production...Janelia Research Campus have provided valuable insight into AAV production. Finally, we are blessed to...vector project team develops methods, performs extensive quality control, generates  educational materials... ship internationally and be ready to provide extensive logistics support for export and shipping on dry...

  7. Q&A with Karen Katz and Carl Paratore, the Newest Members of the Addgene Board of Directors

    Type
    Blog Post
    Published
    Feb. 19, 2019, 1:36 p.m.
    ... Directors brings complementary experience and insight to the now 10 member board at Addgene. Let’s meet...perspective, what do you think are important considerations for the future of open science? What about ... he leads the Internal Audit function and is responsible for bringing a systematic and disciplined approach...Addgene blog Learn more about about Addgene in our "Inside Addgene" blog series Subscribe to the Addgene blog...

  8. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ... kB, then you would be oversized even before considering the need for some manner of polyA. In this scenario...dosing, we advise that users keep titering method consistent across samples within a project. Targeting Your...experiments using PHP.eB in the brain give some insight into codelivery efficiency at different doses. ...best practices’ for model organisms.    Q11: How consistent is expression is when injecting CAP-B10 or CAP-B22...

  9. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...positive population is no higher than 105 signal intensity. Signals any higher than this may not be clearly...comparison, say somewhere between a 104–105 signal intensity. If your samples and controls were processed ...better. The histogram shows peaks of high signal densities, so signal shifts due to upregulation or downregulation... shifts in signal using the mean fluorescence intensity (MFI) of the different populations. MFI is the...

  10. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...using sequence homology is also common. Overlap extension PCR is used to splice together two PCR amplicons... multiple fragments, but these methods can be expensive because they require particular enzymes, some ...recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then joins the...this insertion size, primer synthesis is more expensive than gBlock synthesis, and multifragment assembly...
Showing: 211 - 220 of 729 results