... Originally published Jul 14, 2015 and last updated Sep 16, 2020 by Beth Kenkel.  CRISPR genome editing...Cas12b and CasX. While there are no papers yet published that deliver these Cas proteins with AAV, at just...0908-x  Nishimasu H, Ran FA, Hsu PD, Konermann S, Shehata SI, Dohmae N, Ishitani R, Zhang F, Nureki O (2014...

...(Gootenberg et al., 2017). This system, dubbed SHERLOCK (depicted in figure 1) has been used to differentiate...applications of CRISPR. Since this post was originally published, we've also seen the new development of Cas13b...al., ‘Point-of-care testing for COVID-19 using SHERLOCK diagnostics.’ medRxiv 2020 May 8. Pubmed PMID:...

... incubator). This means any other scientist who wishes to use these cells can see their passage number...immortal life of Henrietta Lacks. New York: Crown Publishers, 2010. 2. Gold, Michael. "A conspiracy of cells...Additional Resources ATCC Animal Cell Culture Guide Thermofisher Guidelines for Maintaining Cultured Cells Invitrogen...

... is a loop in between secondary structure (beta-sheets or alpha helices). An additional requirement is...be generated with a transposon-based strategy (Sheridan, 2002). Avoiding protein over-expression artifacts...PMID: 18454154. PubMed Central PMCID: PMC2853173. Sheridan, Douglas L., et al. "A new way to rapidly create...

...depending on the structure of the TO derivative (Dolgosheina et al., 2014). While 10 derivatives of TO exist...ingredients in the aptamer soup In addition to these established RNA aptamer fluorophores, more are in development...29440634. PubMed Central  PMCID: PMC5811451. Dolgosheina, Elena V., et al. "RNA mango aptamer-fluorophore...

...repressed more   LacZ β-galactosidase activity abolished   lacY Lactose permease inactivated, lactose...plasmid stability recBCD Exonuclease V activity abolished Increased plasmid stability, reduced recombination...r-m+)  MG1655    Wild type K-12 strain; first published sequence of an E. coli K-12 strain. F- λ- ilvG...

...teams: Watson, Crick, & Franklin, Meselson-Stahl, Hershey-Chase...Who was I to dispute history? And boy did...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...myth. The original Dr. Frankenstein from Mary Shelley’s novel worked alone, but even the movie makers ...

...Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents are nonfluorescent...is that binding is covalent, so free dye can be washed away. In addition, labeling contrast can be improved... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...

...around the same time. (The papers were actually published in the same journal on the same day.) This team...lot of terminology in this post! Here’s a quick refresher if you ever lose track of which acronym is which...10.1038/s41556-023-01342-2 Koblan, L. W., Arbab, M., Shen, M. W., Hussmann, J. A., Anzalone, A. V., Doman,...

...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...Yang, W., Leith, A., Daza, R. M., Martin, B., & Shendure, J. (2022). Precise genomic deletions using paired...s41587-021-00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, ...