We narrowed to 554 results for: PPL

...labs, BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today...

... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

...a gene of interest is one of the most common applications of precision genome editing, the remainder of...

... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...

...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...

...proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification of Type VI Cas13d...

... stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP...

... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and...

...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ...

...cleaved by Cas9 and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP...

... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...

...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...

... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

...Scientists can also use electroporation, the application of an electrical charge to cells, to increase...

...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...

...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...

...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin...

...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...

...antibody and content team, we identified what applications should be included and what basic information...

...require there to be a culture condition that can apply enough pressure to select for variants of interest...