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We narrowed to 885 results for: ANT;

Showing: 631 - 660 of 885 results

  1. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...at a lower rate than proteins encoded by highly abundant, charged tRNAs. For example Tuller et al. found...ribosome will move quickly along regions calling for abundant, charged tRNAs but will stall at regions calling...! I should just choose the codon with the most abundant pool of charged tRNAs in my host organism for ...normally found at low abundance in E. coli. The advantage of producing additional tRNAs is that you can ...

  2. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...Cut-to-Mutation distance" affects editing efficiency. One important point to note is that Cas enzymes can continue...The size of the intended edit is the biggest determinant when selecting a type of donor. ssDNA templates...Easi-CRISPR for more details. References and resources Want to know more? Check out the additional resources...PLoS ONE 9(8); (2014). PubMed. Making designer mutants in model organisms. Peng, Y. et al. Development...

  3. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...d microinject into blastocysts to be implanted in pseudopregnant female mice. If you obtained the desired...other homology-directed repair experiments, you’ll want the guide cut site to be as close as possible to...microinjection, i-GONAD is performed directly on pregnant female mice using a small incision that exposes...

  4. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ... monomers, although this can be prevented via antibiotic selection (Crozat, 2014). How to Detect Plasmid...CGE-LIF) for detection and can provide data on the quantity of various topologies and multimers present within...DH5α, HB101, NEB Stable, Stbl3, etc., are recA- mutant strains, and therefore should have reduced frequencies...plasmids causes instability: CoIE1 encodes a determinant essential for plasmid monomerization and stability...

  5. New Coomassie Protocol Video

    Type
    Blog Post
    Published
    Oct. 31, 2023, 1:15 p.m.
    ...your antibody's purity? Check out our latest protocol video, "Coomassie Purity Stain of Recombinant Antibodies...

  6. Plasmids 101: Using Transposons in the Lab

    Type
    Blog Post
    Published
    July 6, 2021, 1:28 p.m.
    ...For example, in bacteria, transposons can move antibiotic resistance genes between plasmids or from plasmids...is active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells...2021). Target site preference and host range are important to consider when selecting a transposon for a ... Sleeping Beauty and piggyBac, which is a big advantage over viral vectors (~5 kB payload for AAV and ...

  7. Which Fluorescence Microscopy Techniques is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...contrast, fluorescence imaging has emerged as the dominant light microscopy contrast technique in modern ...acquired all fluorophores throughout the sample are constantly exposed to light, not just those in the focal...for this to happen.Using red light has a second advantage: red light penetrates deeper into biological tissue...-time monitoring of neuronal activity across substantial 3D volumes. In addition to 2-photon microscopy...

  8. Twenty Years of Sharing Science!

    Type
    Blog Post
    Published
    Jan. 8, 2024, 3:25 p.m.
    ...2!) decades that it's hard to summarize, but we wanted to mark the occasion by trying.  When we started...viral vectors, and in 2022 we added ready-to-use antibodies. Now, we think of ourselves as a repository for...keep an eye on our blog and social media. In the meantime, thank you all so much for being part of our community...

  9. Addgene at SfN23!

    Type
    Blog Post
    Published
    Nov. 9, 2023, 2:15 p.m.
    ...Addgene's booth? Well, first, Addgenies! We'll have an antibody specialist, Amrita, and a viral vector specialist...tons of information about Addgene's ready-to-use antibodies, viral vectors, and, of course, plasmids! Plus...Scientific Writing" on Sunday, from 12 pm - 2 pm. If you want to learn about, and practice, principles of clear...

  10. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...approximate, two-dimensional map. The Baker Lab wanted to create a new fold that is not present in nature...design model at atomic resolution” (Kuhlman and Dantas et al., 2003). That made it a powerful proof of...Hassabis, and John Jumper! References Kuhlman, B., Dantas, G., Ireton, G. C., Varani, G., Stoddard, B. L....

  11. Deck the Lab 2022!

    Type
    Blog Post
    Published
    Jan. 17, 2023, 2:15 p.m.
    ... my assay at like 23:00, so I decided to make a giant snow angel for biologists in our building. Unfortunately...2022   Congratulations to all our #DeckTheLab contestants!           View this post on Instagram                     ... labs with everything from Falcon tube trees to giant paper mache Krampus performing routine lab maintenance...

  13. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...depositor, but it can also be valuable data to share in grant proposals or to demonstrate impact in annual reports...features of dashboards are (i) easy access to important numbers and trends, (ii) user interaction with...metrics!  About the Author Joachim Goedhart is an assistant professor at the Section of Molecular Cytology...

  14. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO, deposited...offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO.  This is...bistable nature, it enables inhibition of synapses distant to the site of injection with high temporal precision...

  15. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...peek in case you missed them. July 2016 was a fantastic month for the Addgene blog. We achieved roughly...editing when compared to the most commonly used Cas9 variant, SpCas9. These properties, combined with other ...in the post, may make Cpf1 the preferred CRISPR variant going forward.   Special Delivery Fluorophore Targeting...

  16. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...been working with Seeding Labs to provide plasmid grants to researchers in developing countries. Today we...announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena O. Duedu...excited to discover what new and creative ideas applicants will propose for plasmids from the Addgene community...

  17. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ...into next year we’d like to know what topics you want to learn more about. Our post on “Making Your Own...Finally, we are always looking for guest bloggers who want to share their research with the community, so email...Mentoring Support By Joanne Kamens Plasmids 101: Antibiotic Resistance Genes By Marcy Patrick Plasmids...

  18. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...fresh new friend: Antibody of the Day!  Figure 4: The plasmid, viral prep, and antibody of the day While...others may be less obvious — but every change is meant to make your experience on our website better so...

  19. More Data for You: Find Articles Citing Addgene Plasmids

    Type
    Blog Post
    Published
    June 16, 2015, 2:15 p.m.
    ...programmable DNA-binding domains" publication.  If you want to see this new feature in action, click here to...plasmid of interest, which may help you identify relevant protocols and experimental data Find other related... an article that used a plasmid from Addgene, we want to hear about it so we can link to it in the "Articles...

  20. Download Addgene’s Science Career Guide, Plus 8 Career Tips from Addgenies

    Type
    Blog Post
    Published
    Feb. 26, 2019, 1:40 p.m.
    ...Product Management “I knew very early on that I wanted to be an artist/designer. But there were a few ...Campbell, and read about his "follow your bliss' mantra and never forgot it. Usually when I see inspiring...Designer On the job search “Always go for the job you want, even if you don’t think you’re qualified. The worst...

  21. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ...are some additional features. The first and most imporant is that, upstream of the Cbh promoter, this plasmid...GFP-tagged and un-tagged versions of SpCas9 and further variants replace SpCas9 with SpCas9n (nickase), which nicks...homology directed repair. You can find all of these variants, as well as links to the Zhang Lab's Nature Protocols...

  22. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...focus on an aspect of communication especially important for a manager – getting feedback from the team... This can take practice and training. This is important because it creates a culture of engagement in ...  Seeking feedback frequently One of the most important paths of information flow is the one that brings...

  23. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    Published
    Nov. 27, 2013, 2:54 p.m.
    ...competition featuring a selected ingredient. Anyone who wants to participate brings in a dish that features that... for our website. Free lunch is provided for participants during the Hack Days, and we’ve seen great success...easy as possible. However, I think it’s just as important to take a break from work to share time with the...

  24. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ...activities. Working with proteins requires a substantial amount of properly folded, relatively pure protein...al. "The pCri system: a vector collection for recombinant protein expression and purification." PloS one..., Zachary T. Britton, and Anne S. Robinson. "Recombinant protein expression and purification: a comprehensive...

  25. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...defend the thesis, etc. Photography is critically important in many fields of biology and I believe it could... use of x-rays. His haunting images have been enchanting people for years and have gathered much acclaim...method to explore the inner structures of delicate plants and other organisms without destroying the original...

  26. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...studying COVID-19. “Open scientific sharing is important for creating research synergies and accelerating...believe that by coming together, we can create a significant impact on both clinical care and public health..., scientific sharing will remain an extremely important part of the future of research. To find other ...

  27. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...It was immediately clear that Addgene wanted to ensure applicants understood what the expectations were... with the different types of issues that were important to this function. With the welcoming environment...

  28. Supporting Rare Disease Research with BeHeard 2017

    Type
    Blog Post
    Published
    July 20, 2017, 2:30 p.m.
    ... from rare diseases by connecting patients to important resources and promoting research for rare disease...disease. This year, Addgene will be providing plasmid grants worth $2,000 each to two labs through the BeHEARD...physicians and scientists.  Although the form of malignant mesothelioma that affects the lining of the lungs...

  29. Future of Research Conference - Remarkable Opening Session

    Type
    Blog Post
    Published
    Oct. 7, 2014, 3 p.m.
    ...Conference at Boston University last week.  This fantastic city-wide effort was led by two amazing postdocs...practices of indirect costs.  The peer review and grant awards systems need to be radically overhauled.  ...alternate training needs to be provided to take advantage of all the possible science careers. She advocated...
Showing: 631 - 660 of 885 results