We narrowed to 842 results for: Eras

...the grantor’s requirements as well as state and federal regulations. A grant professional’s job duties ...Dr. Cook served as a principal investigator for several research grants. One of the benefits of being a...management, and program evaluation are highly transferable. Use your experience and your passion(s) to ...

...scientific mentor is typically a professor who operates a research group. Within that research group, ...everything! The bare minimum should be taking notes on general protocol steps and other tips and tricks your mentor...my dissections go smoother next time?” or more generally “How am I doing? Can you tell me what’s going ...

...sites differ at the nucleotide level but share an overall structure of 13 base pair palindromic repeats separated...efficient The first version of FLP discovered has a temperature optimum of 30 °C and is therefore inefficient...led to the identification of FLPe which has a temperature optimum of 37 °C (Buchholz, Angrand, and Stewart...

...are below the new exemption level (the current average postdoc salary is estimated at around $45,000, ...tests must be met. In the Department of Labor’s general guide to the effect on higher education, you can...Perez, Fair Pay for Postdocs: Why We Support New Federal Overtime Rules, made clear that institutional arguments...

... with low affinity for ligands. When integrins interact with extracellular ligands, their ectodomains ...sites and do not interfere with integrin function. Overall, the synthetic rAbs demonstrate remarkable specificity...cells. What’s next IPI is working with Addgene to accelerate biomedical research by distributing antibodies...

...still go horribly awry when several factors aren’t taken into consideration. 1. Always focus on the science...equipment that you use in your current lab first. Generally, major equipment can be defined as equipment with...

...with a 2:1 BSc in Molecular Biology (roughly a B average in the American system), in 2009 (at the beginning...structured and controlled. I worked from Standard Operating Procedures (SOP’s) and every item I tested was...sustainable. After working at this company for several years, I was ready for a change.  Working abroad...

...resonance energy transfer (FRET) is a biophysical interaction between two fluorophores. When the first fluorophore...& Zhang, 2006), quantifying antibody/antigen interactions (Saraheimo et al., 2013), and visualizing structural...different biomolecules you’d like to test for interaction. In fact, targeting a FRET fluorophore to a biomolecule...

...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...gRNAs, with four being the ideal number. First you generate four unique kanamycin-resistant plasmids, each...each unused promoter. This step is necessary to generate all of the overhangs needed for the final ligation...

...Toolbox for quickly generating zebrafish transgenic lines to study neurodegenerative diseases. Listen to...

...restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike.... L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports...

...hydroelectric in nature. The operation of -80C and -20C freezer temperatures, biosafety cabinet ventilation...

...viral lots. Aligning with Addgene’s mission to “accelerate research and discovery by improving access to...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...

...outlets meaning you’ll have the opportunity to interact with and discuss your work with many different...applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp...

...department at MIT. Crystal now works on protein therapeutics at Novartis - listen to learn more about Crystal...in industry. The importance of recognizing and leveraging your unique skills set while also adapting to...

...Genetics Institute in Cambridge, MA and Sequana Therapeutics in San Diego, CA. She earned a B.S in Biology...Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth was an investment...

...visualize the aggregated data. Plotting the data in interactive figures is a great step up, but I needed a way...access to important numbers and trends, (ii) user interaction with the data, and (iii) the ability to easily...

...We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO,... is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO....

...perturbations to how CRISPR has made it much easier to generate mouse models.   Cpf1 Update: Comparison to Cas9...: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison...

...the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains a mutation or modification...be included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide...

...including yeast and E. coli engineering, microbial cooperation, and antibiotic production. Chapter 5: The future...before spending time in biotech screening direct interactions between drugs and promoters of oncogenes. He...

..., Addgene remains steadfast in our mission to accelerate research and discovery by improving access to...funding transitions. Enabling resource sharing: Our operating model allows researchers to access authenticated...

...who was excited about “Seeing a real lab!” And several of our visitors made sure to tell us what we already...already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The students also got to ...

..., along with links to the relevant scientific literature.  -In light of the current Ebola crisis, Science... present, and future, don’t miss Carl Zimmer’s coverage on the subject in the New York Times and on his...

...modification in monkeys is invaluable for the generation of human disease models." Addgene Outreach Scientist... References, Protocols, and more References: Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated...

... done a really good job at figuring out how to operate during the pandemic, but it’s important to develop...on-site work?” and “How do you start to expand operations a little bit?”    What are you doing to enjoy...

...plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post. 9. Droplet...subset of the fluorescent protein family and they generate color! They've been adapted for many molecular...

...AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...

...Make sure you will enjoy being in that lab and interacting with the lab members.”- Jennifer Tsang, Science...Addgene blog Read all careers blog posts Read our transferable skills guide Resources on Addgene.org Find...

... bind specific genes and turn them on or off, literally at the flip of a (blue) light switch. Zhang’s ...Views Andreas Möglich and Peter Hegemann described several advantages of the new system: "First, the light-responsive...