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Showing: 631 - 660 of 824 results

  1. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO,... is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO....

  2. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...visualize the aggregated data. Plotting the data in interactive figures is a great step up, but I needed a way...access to important numbers and trends, (ii) user interaction with the data, and (iii) the ability to easily...

  3. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...perturbations to how CRISPR has made it much easier to generate mouse models.   Cpf1 Update: Comparison to Cas9...: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison...

  4. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains a mutation or modification...be included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide...

  6. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ..., Addgene remains steadfast in our mission to accelerate research and discovery by improving access to...funding transitions. Enabling resource sharing: Our operating model allows researchers to access authenticated...

  7. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...who was excited about “Seeing a real lab!” And several of our visitors made sure to tell us what we already...already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The students also got to ...

  8. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ..., along with links to the relevant scientific literature.  -In light of the current Ebola crisis, Science... present, and future, don’t miss Carl Zimmer’s coverage on the subject in the New York Times and on his...

  9. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...modification in monkeys is invaluable for the generation of human disease models." Addgene Outreach Scientist... References, Protocols, and more References: Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated...

  10. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...

  13. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post. 9. Droplet...subset of the fluorescent protein family and they generate color! They've been adapted for many molecular...

  14. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ... bind specific genes and turn them on or off, literally at the flip of a (blue) light switch. Zhang’s ...Views Andreas Möglich and Peter Hegemann described several advantages of the new system: "First, the light-responsive...

  15. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...spooky science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their...climb upwards until they reach a point where the temperature and humidity favors fungal growth. The ants bites...

  16. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...reunions in various wildernesses and enjoys “three generations walk[ing] together in the Arboretum and other... all work and no play Although hard work and perseverance are required to achieve success in the lab, ...

  17. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining fluorescent...proteins, but also investigate protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn...

  18. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...transcriptional regulators. In this system, Cas9 generates a double-stranded DNA break at the genomic loci... E. coli MG1655 genome with an NGG PAM motif, averaging 19 targets per gene. The lab has previously used...

  19. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...Twitter lists were searched for scientific titles generated from the United States Standard Occupational Classification...tweeted science domain by scientists was Nature. Overall scientists tweet URLs with scientific domains <...

  20. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...this is vital to ensuring that our services help accelerate your research. We know you don’t have time to...answer that you’re looking for, or you just have a general question or concern about the answers we provide...

  22. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...Genome wide mouse CRISPR library The Yusa lab has leveraged the CRISPR system to target all 19,150 mouse protein...Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (...

  24. SunTag and Fluorescent Imaging

    Type
    Blog Post
    Published
    March 28, 2017, 2:30 p.m.
    ...mitochondria without organelle toxicity. First generation (v1) SunTag is expressed at very low levels due...Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ...

  25. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ... Oliveira. Bricking Science is an idea built, literally, 'brick-by-brick' to introduce people all around... video where he/she/it/something is captured on camera: Unfortunately, unless they’ve worked in a lab...

  26. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...easier, cost effective and faster; this has helped accelerate scientific discoveries.” Occasionally, a plasmid...because, as UNC’s Bryan Roth says, Addgene has “literally enabled sharing with 100's of investigators worldwide...

  27. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...vary. In the PAM-out configuration, D10A tends to generate small deletions, while H840A is biased towards... likely due to the distinct overhang patterns generated by the nickases; D10A creates 5’ overhangs and...

  29. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ...sequence as the scaffold. gRNA: "Guide RNA" is a general term referring to the RNAs used by Cas proteins...order to cut DNA, whereas some mismatches can be tolerated outside this region. Conclusion Ready for some...

  30. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type
    Blog Post
    Published
    Sept. 12, 2023, 1:15 p.m.
    ...tools, but here are a few stand-alone sites for general primer design, as well as primer design for different...cloning applications. Primer3 and Primer3Plus: For general primer design Design primers for Goldengate cloning...
Showing: 631 - 660 of 824 results