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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 834 results for: Eras

Showing: 631 - 660 of 834 results

  1. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...multiplex gRNAs, some being: Using dual nickases to generate a knockout or edit. This can help reduce off-target...gRNAs, with four being the ideal number. First you generate four unique kanamycin-resistant plasmids, each...each unused promoter. This step is necessary to generate all of the overhangs needed for the final ligation...

  3. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...restriction digested with PstI or EcoRV, these plasmids generate 100 bp or 1 kb DNA ladders, respectively. Unlike.... L., & Tan, S. (2017). The pPSU Plasmids for Generating DNA Molecular Weight Markers. Scientific Reports...

  4. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...hydroelectric in nature. The operation of -80C and -20C freezer temperatures, biosafety cabinet ventilation...

  5. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...viral lots. Aligning with Addgene’s mission to “accelerate research and discovery by improving access to...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...

  6. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...outlets meaning you’ll have the opportunity to interact with and discuss your work with many different...applying CRISPR for mouse genome editing. Post: Generating Mouse Models Using CRISPR/Cas9   Erik L. Snapp...

  8. New Viral Vectors - Summer 2024

    Type
    Blog Post
    Published
    July 9, 2024, 1:15 p.m.
    ...We've also added AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO,... is now offering AAV1 and AAV5 preparations of several variants of a new optogenetic tool called PdCO....

  9. Visualizing Your Plasmid Request Data

    Type
    Blog Post
    Published
    April 11, 2023, 4 p.m.
    ...visualize the aggregated data. Plotting the data in interactive figures is a great step up, but I needed a way...access to important numbers and trends, (ii) user interaction with the data, and (iii) the ability to easily...

  10. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...Genetics Institute in Cambridge, MA and Sequana Therapeutics in San Diego, CA. She earned a B.S in Biology...Addgene as the Chief Financial Officer and Chief Operating Officer. Prior to Addgene, Kenneth was an investment...

  11. Editor's Choice, July 2016

    Type
    Blog Post
    Published
    Aug. 5, 2016, 3 p.m.
    ...perturbations to how CRISPR has made it much easier to generate mouse models.   Cpf1 Update: Comparison to Cas9...: Addgene at the ASM Microbe 2016 Conference Generating Mouse Models Using CRISPR/Cas9 Cpf1 Update: Comparison...

  12. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...the insert. pBACKBONE-Flag-hGene-EGFP Other considerations  If your insert contains a mutation or modification...be included in the plasmid name. Mutations are generally listed as the amino acid change and not a nucleotide...

  13. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ..., Addgene remains steadfast in our mission to accelerate research and discovery by improving access to...funding transitions. Enabling resource sharing: Our operating model allows researchers to access authenticated...

  15. MassBioEd Career Exploration Day at Addgene

    Type
    Blog Post
    Published
    Nov. 13, 2015, 3:30 p.m.
    ...who was excited about “Seeing a real lab!” And several of our visitors made sure to tell us what we already...already knew about our Shipping Operations Coordinator: “Kory was awesome!”. The students also got to ...

  16. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ..., along with links to the relevant scientific literature.  -In light of the current Ebola crisis, Science... present, and future, don’t miss Carl Zimmer’s coverage on the subject in the New York Times and on his...

  17. Keeping Up With CRISPR/Cas9

    Type
    Blog Post
    Published
    March 11, 2014, 5:55 p.m.
    ...modification in monkeys is invaluable for the generation of human disease models." Addgene Outreach Scientist... References, Protocols, and more References: Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated...

  19. What's New in CRISPR - Spring 2019

    Type
    Blog Post
    Published
    March 21, 2019, 12:47 p.m.
    ...AacCas12b) exhibits optimal DNA cleavage at 48 °C, a temperature incompatible to genome editing in human cells...is a single step gRNA multiplexing system that generates vectors containing high numbers of gRNAs in one...

  21. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...plasmid in TE, Tris, or water? And what about temperature? Find the details in this blog post. 9. Droplet...subset of the fluorescent protein family and they generate color! They've been adapted for many molecular...

  22. Let There Be LITE Plasmids

    Type
    Blog Post
    Published
    Nov. 21, 2013, 3:56 p.m.
    ... bind specific genes and turn them on or off, literally at the flip of a (blue) light switch. Zhang’s ...Views Andreas Möglich and Peter Hegemann described several advantages of the new system: "First, the light-responsive...

  23. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    Published
    Oct. 31, 2019, 1:06 p.m.
    ...spooky science stories Zombie ants Ophiocordyceps unilateralis is a fungus that infects ants and controls their...climb upwards until they reach a point where the temperature and humidity favors fungal growth. The ants bites...

  24. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...reunions in various wildernesses and enjoys “three generations walk[ing] together in the Arboretum and other... all work and no play Although hard work and perseverance are required to achieve success in the lab, ...

  25. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ... successful transformants. Addgene distributes several ready-to-use, modular plasmids, combining fluorescent...proteins, but also investigate protein-protein interactions via FRET. Sidae Lee, Wendell Lim, and Kurt Thorn...

  26. Who Gives a Tweet? 9 Facts About Scientists on Twitter

    Type
    Blog Post
    Published
    June 26, 2017, 1:31 p.m.
    ...Twitter lists were searched for scientific titles generated from the United States Standard Occupational Classification...tweeted science domain by scientists was Nature. Overall scientists tweet URLs with scientific domains <...

  27. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ...transcriptional regulators. In this system, Cas9 generates a double-stranded DNA break at the genomic loci... E. coli MG1655 genome with an NGG PAM motif, averaging 19 targets per gene. The lab has previously used...

  28. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    Published
    Dec. 10, 2015, 3:30 p.m.
    ...this is vital to ensuring that our services help accelerate your research. We know you don’t have time to...answer that you’re looking for, or you just have a general question or concern about the answers we provide...

  30. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...Genome wide mouse CRISPR library The Yusa lab has leveraged the CRISPR system to target all 19,150 mouse protein...Koike-Yusa H, Li Y, Tan E-P, Del Castillo Velasco-Herrera M, Yusa K. Nature Biotechnology. 2013 Dec 23. (...
Showing: 631 - 660 of 834 results