We narrowed to 750 results for: Tro;

1. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

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   Blog Post

   Published

   Feb. 18, 2015, 3:09 p.m.

   ...transfect CRISPR plasmids into MEL cells by electroporation, sort your transfected cells via FACS, primer...

2. "What Makes a Good Mentor?" and 6 More FAQs About Science Mentoring

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   Blog Post

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   Dec. 17, 2013, 2:47 p.m.

   ...enough to accommodate economic difficulties, family trouble, or health and mental problems that get in the ...

3. Tag Your Favorite Yeast Genes with Ease

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   Blog Post

   Published

   Nov. 19, 2013, 2:37 p.m.

   ... site of insertion. The linear PCR product is introduced into the cell by direct transformation. A given...

4. New Norepinephrine Indicators: nLightG and nLightR

   Type

   Blog Post

   Published

   Dec. 12, 2023, 2:15 p.m.

   ...Kagiampaki et al. found nLight is suitable for in vitro, ex vivo, and in vivo experiments, and nLightG was...

5. Working 9-5: an Addgene Co-op

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   Blog Post

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   June 2, 2022, 2:15 p.m.

   ...responsibilities Through this training, I was gradually introduced to Addgene as a company and given more details...

6. Spurred by COVID-19, Addgene Bridges Plasmid Sharing Between Academia and Industry

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   Blog Post

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   June 23, 2020, 1:15 p.m.

   ...synthesize new plasmids quickly and Addgene’s quality control and archiving system allowed us to handle and distribute...

7. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

   Type

   Blog Post

   Published

   Aug. 13, 2014, 3:49 p.m.

   ...disease, and cancer in particular. They recently introduced the SpyTag/SpyCatcher system, based on a protein...

8. Year of the Camelid: Antibody Style

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   Blog Post

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   May 9, 2024, 1:15 p.m.

   ...03676-z  More resources on the Addgene blog Introduction to Antibodies  Secondary Nanobody Toolbox No...

9. Embracing Serendipity: A Crucial Element in the PhD Journey

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   Blog Post

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   April 25, 2024, 1:15 p.m.

   ...Why serendipity matters Serendipity is not just a stroke of luck; it's a mindset that can lead to unexpected...

10. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

    Type

    Blog Post

    Published

    Sept. 12, 2023, 1:15 p.m.

    ...Benchling: While you might think of Benchling as an electronic lab notebook, it also has a suite of molecular...

11. Antibodies 101: Fc Effector Functions

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    Blog Post

    Published

    May 18, 2023, 1:15 p.m.

    ...resources on the Addgene blog Antibodies 101: Introduction to AntibodiesAntibodies 101: IsotypesAntibodies...

12. Cellosaurus: A Cell Line Information Database

    Type

    Blog Post

    Published

    Feb. 21, 2023, 2:15 p.m.

    ...This post was co-authored by Susanna Stroik and Rachel Leeson. Here at Addgene, we like to help share...

13. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type

    Blog Post

    Published

    March 15, 2018, 12:59 p.m.

    ...subsequent experiment, they were also able to introduce a longer insertion (mCherry) using IDT Megamer...

14. ReFigure: Save Scientific Figures into Dashboards and Share Your Insights

    Type

    Blog Post

    Published

    Dec. 13, 2017, 3:20 p.m.

    ...replications as in this ReFigure about whether Natronobacterium gregoryi Argonaute has any DNA editing functionality...

15. Bringing Sustainable Practices to the Lab: Recycling

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    Blog Post

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    Feb. 14, 2019, 2:35 p.m.

    ... these boxes a lot when receiving temperature-controlled reagents. Expanded polystyrene will likely end...

16. Bricking Science: Portraying Scientific Reality Through LEGO

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    Blog Post

    Published

    Nov. 29, 2016, 3:30 p.m.

    ...an idea built, literally, 'brick-by-brick' to introduce people all around the world to the lives of researchers...

17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type

    Blog Post

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    June 5, 2017, 3:19 p.m.

    ...all the extra services we provide like quality control, curation, technical service and open-access educational...

18. Sensing Neuronal Dopamine

    Type

    Blog Post

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    July 30, 2019, 12:59 p.m.

    ...reinforcement learning, decision making, and motor control.  What do we understand about Dopamine? DA is ...

19. Hot Plasmids - October 2022

    Type

    Blog Post

    Published

    Oct. 6, 2022, 1:15 p.m.

    ...represent a long-sought tool for researchers to control K+ gradients and can be used as a potent optogenetic...

20. Running for Rare Disease, Running for FOP, Running for AJ

    Type

    Blog Post

    Published

    Sept. 15, 2016, 2:30 p.m.

    ...Oath says: "In fearful day, in raging night, With strong heats full, our souls ignite, When all seems lost...

21. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post

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    June 30, 2015, 2 p.m.

    ...laboratory setting, transposons can be used to both introduce genes into an organism’s genome (see figure) and...

22. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

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    Blog Post

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    Jan. 15, 2015, 1:50 p.m.

    ...Addgene was mentioned specifically in Dr. Zhang's introduction. His willingness to share reagents so freely...

23. 10 Ways to Share Your Science!

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    Blog Post

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    May 13, 2014, 8:34 p.m.

    ... distant island. He got a little money for his trouble, too. 7)   Reach out to science writers in your...

24. Advice for Choosing a Research Project

    Type

    Blog Post

    Published

    April 17, 2014, 2:45 p.m.

    ...by your own subject - maybe something used in astrophysics can be applied to fungal biology.” Connie Cepko...

25. The Stingy Scientist: How the Baby Gel Box Was Born

    Type

    Blog Post

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    April 15, 2014, 3:11 p.m.

    ... your fingers in during a run or risk minor electrocution.  These days you can buy small gel boxes, but...

26. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

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    Jan. 30, 2014, 3:29 p.m.

    ...disrupt genes at the chromosomal level. Scientists introduce an antibiotic resistance cassette within the coding...

27. Addgene Begins Distribution of Recombinant Antibodies

    Type

    Blog Post

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    March 28, 2022, 4:30 p.m.

    ...Educational Resources The Basics of Western Blotting Introduction to Immunofluorescence Addgene’s Antibody Guide...

28. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    Published

    May 25, 2021, 2:29 p.m.

    ...current antibody plasmid collection) and we plan to introduce affinity reagents in protein format within the...

29. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...bright, monomeric green fluorescent protein. In vitro, mWasabi is 1.6-fold brighter than EGFP and is photostable...

30. What Do I Do Now? Academic v. Non-Academic Career Decisions

    Type

    Blog Post

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    March 9, 2017, 3:40 p.m.

    ...do quality science on interesting topics Having strong non-science skills is important for success Must...