We narrowed to 554 results for: ESP

1. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

   Type

   Blog Post

   Published

   Jan. 11, 2017, 3:30 p.m.

   ...the postdoctoral population which keeps growing despite a lack of available academic positions.   Many...

2. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...toxin-antitoxin system encoded by the ccd operon, which is responsible for plasmid maintenance during cell division....

3. Antibodies 101: Conjugation

   Type

   Blog Post

   Published

   March 26, 2024, 1:15 p.m.

   ...cover all of the possible antibody conjugates (especially if you start to consider clinical applications...

4. Gendered Innovations: Why Does Sex of the Cell Matter?

   Type

   Blog Post

   Published

   July 21, 2016, 2:30 p.m.

   ... researchers wanting to consider sex is cost. Especially in animal research, they worry about needing ...

5. Lighting Up Cell Signaling with Photoswitchable Kinases

   Type

   Blog Post

   Published

   July 25, 2017, 1:20 p.m.

   ...phosphorylated MEK1/2 levels, suggesting PP1/PP2A is responsible for this MEK1/2 negative feedback. 3. Studying...

6. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...are established monomeric and dimerizing FPs respectively, are used as positive and negative controls ...

7. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ...negative regulation by using a promoter that is responsive to a repressor protein, the increase in expression...

8. RaPID Detection of RNA-protein Interactions

   Type

   Blog Post

   Published

   Aug. 16, 2018, 12:40 p.m.

   ...is characterized by point mutations in the Iron Response Element (IRE) RNA motif of the ferritin light ...

9. Plasmids 101: The protein expression toolbox

   Type

   Blog Post

   Published

   Feb. 16, 2023, 7:05 p.m.

   ...Tetracycline-dependent promoters are engineered by placing tet response elements upstream of a minimal promoter. These...

10. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...triphosphate in the gRNA, reduce the innate immune response associated with gRNA introduction (Allen, et al...

11. Antibodies 101: Selecting the Right Antibody

    Type

    Blog Post

    Published

    March 15, 2022, 1:15 p.m.

    ...from a vendor that you use frequently, with a responsive and knowledgeable technical support team, is ...

12. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ... particles for delivery into mammalian cells, especially when fused to effector proteins for gene activation...

13. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...Furthermore, RNases, or enzymes that degrade RNA, are especially hardy proteins that are found in and on everything...

14. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ...bp gRNA target sequence and its 8-bp barcode, respectively. Once cloned into the storage vector, the pooled...

15. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

    Type

    Blog Post

    Published

    Nov. 24, 2015, 3:30 p.m.

    ... These systems, known as T-DDOG and Cre-DOG, respectively, repurpose popular GFP reporter lines for more...

16. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...growing dialogue on the state of a system that, despite all of its successes, has many deep-rooted flaws...

17. Plasmids 101: Golden Gate Cloning

    Type

    Blog Post

    Published

    Aug. 27, 2015, 2:30 p.m.

    ...and minuses, but Golden Gate cloning has been especially useful within both the synthetic biology and ...

18. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type

    Blog Post

    Published

    May 5, 2015, 3:54 p.m.

    ... shown me that I’m still in the dark ages with respect to my organization strategy. There are a number...

19. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...reproductive, developmental, metabolic, and immune response programs. NRs also encompass one of the most successful...

20. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ... along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality. Since...