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We narrowed to 836 results for: Eras

Showing: 421 - 440 of 836 results

  1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type
    Blog Post
    Published
    May 3, 2018, 1:48 p.m.
    ...cleavage promotes collateral cleavage of other RNAs. As with other Cas13s, this collateral cleavage does ...and Yan et al. describes new Cas13d enzymes that average only 2.8 kb in size and are easy to package in ...possibilities. To identify Cas13d, both groups used very general bioinformatic screens that looked for a CRISPR ...accessory protein RspWYL1 increases both targeted and collateral RNA degradation by RspCas13d. RspWYL1 also increased...

  2. Screening for Successful Genome Editing with Digital PCR

    Type
    Blog Post
    Published
    March 30, 2017, 2:30 p.m.
    ...successful mutation of your favourite gene, there are several different methods available. Thankfully, there ...validation, such as this "CRISPR 101" post. Next-generation sequencing technologies are the gold standard...emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays that will...desired genome edit. Since exploitation of NHEJ to generate translational reading frame-altering indels for...validation methods As mentioned, these assays have several major advantages over the commonly employed mismatch...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...publication in a scientific journal requires several considerations. First, it is important to establish the...whether your paper is in scope for the journal’s coverage. These guidelines also provide information on ...delay publication and manuscripts may go through several rounds of revision before acceptance. F1000Research...prerequisites of the journal. Ask colleagues (preferably native English speakers) to proofread your manuscript...

  4. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...means that F(ab’)2 fragments may have overall stronger interaction with their antigens than a Fab or Fab...the protein will interact with other components of your experiment. These considerations will help you narrow...secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ...

  5. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...repeating before you can finally dive deep into generating the data you’re excited about. When we were ...increase dramatically if you are piecing together several PCR products together. And this assumes you did...cost per stab has increased 50% since 2019, while overall inflation of USD has gone up 19.8% in the same ...making one plasmid at a time, try constructing several plasmids in parallel. That way, you can run one...help scientists save even more!) If you assume an average of 5 hours of hands-on time per plasmid, Addgene...

  6. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...secondary considerations to validation, application specificity, and experimental considerations - no other...rarely work for every assay. Antibodies that are generated against a linear antigen tend to work very well...permeabilization will influence how the antibody interacts with its target and consequently the quality of...isolate an antibody’s immune complexes and confirm interacting partners by mass spectrometry (Uhlen, 2016). ...

  7. RNA Extraction Without A Kit

    Type
    Blog Post
    Published
    March 24, 2020, 1:15 p.m.
    ...on DNA purification without a kit that outlined several reasons why doing something without a kit has advantages...everything, including your skin. Here are some general tips for working with RNA, even if you are using...method, so biotechnology companies have marketed several products that help to further streamline this process...quantitative PCR to assess gene expression. There are several things that researchers can do to combat this issue...and proteins. Lastly, using a DNase (there are several DNase enzyme products on the market to choose from...

  8. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...studied a synaptic protein that is linked to neurodegeneration. The level of this protein is decreased in...method for the visualization of protein-protein interactions in situ. In the standard PLA assay, primary ...endogenous proteins of interests, suspected to interact, are added to a cellular sample. Both antibodies...visualization of the location of the protein-protein interaction. To study protein levels in situ, the Schumann...protein plasmids Use FRET to study protein-protein interactions Deposit plasmids with Addgene! ...

  9. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type
    Blog Post
    Published
    Dec. 8, 2016, 3:30 p.m.
    ... cloning, you’re probably aware that there are several methodologies currently available for approaching... and Gateway® cloning technologies, as well as several others. Each method is unique and relies on specific...to propagate because CcdA wasn’t available to counteract the toxin. If, however, an investigator were ...answer lies in special strains of E. coli that tolerate the expression of the toxin gene. One such strain...plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes.” J Mol Biol. 1992 Aug 5;226(3)...

  10. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type
    Blog Post
    Published
    April 12, 2016, 2:30 p.m.
    ...genome editing in large-scale has led to the rapid generation of genome-wide gRNA libraries that knock out ...themselves incredibly useful for the efficient generation of individual (and multiple) genetic perturbations... al., 2015; Wong et al., 2016). CombiGEM uses iterative one-pot reactions with straightforward restriction...functionally characterize combinatorial gene knockouts generated via multiplexed gRNA expression (Wong et al., ...gRNA targeting sequences, you can then readily generate your barcoded gRNA library sequences via oligo...

  11. How to Prepare for an Industry Interview

    Type
    Blog Post
    Published
    July 9, 2020, 1:15 p.m.
    ...interviews, however, were more structured. I met with several individual and/or small groups, usually starting...interviewer,” you can find lists that have lots of general questions about job responsibilities, performance... an opportunity to demonstrate technical and transferable skills that make you qualified for the position...position. Highlight the outcomes or deliverables of your work, such as improving a process or procedure, ... pursuing a particular line of research is a deliverable. Find 10 steps to a perfect science talk Be prepared...

  12. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...recognize multiple isotypes from a species, like a general anti-mouse antibody. Others recognize specific ...better choice for a mouse monoclonal IgG2a than a general anti-mouse IgG. In a broadly specific secondary...different species.      Table 1: Isotype considerations when choosing antibodies   Complexity is...tend to be complex with a variety of proteins interacting. Experiments tend to be equally complex with ...narrowly specific secondary antibodies rather than generally reactive ones. And make sure to check for any ...

  13. Hot Plasmids February 2024

    Type
    Blog Post
    Published
    Feb. 20, 2024, 2:45 p.m.
    ...dimeric nature can interfere with some applications. Several labs have been working to produce monomeric versions... under CC-BY license.   The Miyawaki lab generated a series of StayGold mutants and identified a ...mentioning that Kiryl Piatkevich and colleagues generated yet another monomeric StayGold variant (Piatkevich...primates. The authors used a multispecies approach to generate and screen a library of AAV9 variants (Chuapoco... brains. Figure 3: Overview of library generation and screening that led to identification and ...

  14. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    Published
    May 24, 2018, 1:20 p.m.
    ... preparation of the tissue sample, several washes, and temperature changes to visualize the expression...to study gene expression and functions of key regeneration-related genes in four inexpensive and commercially...linearized DNA templates which they can easily generate off of a circular plasmid via PCR. Typically, ...expression is activated through the  induction of T7 polymerase encoded in the E. coli genome (usually controlled...

  15. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...Collection at Coriell. More information and images generated from these cell lines can be found in the Cell...functional aspects of the protein gleaned from the literature and engagement with researchers who study the...find out more about gene edited hiPS cell lines generated using the Allen Institute for Cell Science Plasmid...Drosophila cells, and C. elegans. In addition to generating fluorescent protein fusions, plasmids are available...find out more about gene edited hiPS cell lines generated using these plasmids, please visit the Cell Catalog...

  16. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

    Type
    Blog Post
    Published
    Sept. 13, 2016, 2:30 p.m.
    ...Alberts et al, PNAS 2014; Taylor, eLife, 2016). Several Future of Research (FoR) symposia held across the...and Molecular Biology/ FoR Hack Day during which several projects were initiated. Recent developments promise...the passing and upcoming implementation of the Federal Labor Standards Act (FLSA) which mandates that ...different departments across the university and alterations to the student-university relationship, along...2. Taylor, Justin Q., et al. “Avoiding a lost generation of scientists.” eLife 5 (2016): e17393. Pubmed...

  17. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...facilitate effective teamwork, manage day to day operations within their workplace, and pursue leadership...need regular re-direction and alignment with the overall mission of the NPA. You must accommodate many different...learnt to try and bring a group of people to an overall consensus and developed negotiation skills that...would be useful in many careers. I learnt that the overall voice may not always be perfectly aligned with ...to think about their career options, gaining transferable skills such as leadership skills can only assist...

  18. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...how best to prepare. Maybe you’re a presentation veteran, but have difficulty answering questions. Or perhaps... virus of interest causes cancer, as well as therapeutic strategies to target it, than you typically would...topics,” so be sure to be well-read on the recent literature in your field. Take your time: We often get so...making it difficult to see the actual poster or interact with the presenter. If you don’t have time to ...at the University of Arizona. She studies the interaction between Human Papillomavirus and the innate immune...

  19. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ...controversy, and the interactions of organic molecules like proteins with magnetic fields generally appears to ...magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random mutagenesis and screening...behavior has already been successfully reproduced by several other labs. Andrew and Maria expect that further-improved...tools.  Embracing science in the open The York Lab operates with a strong commitment to exploration and collaboration...

  20. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...be able to tell you the laser configuration. In general, if two fluorescent markers are read by the same...to become a perpendicular vertical line.     In general, most spillover between samples can be fixed with...anyone just starting to use flow cytometry, it’s generally recommended to start compensating with single-... compensation panels below each plot.  The general rules of compensation apply to your experimental...research background in CD8+ T cells and cancer immunotherapies. She is currently a research technician in ...
Showing: 421 - 440 of 836 results