We narrowed to 566 results for: FIE

1. New and Upcoming Viral Vectors - September 2019

   Type

   Blog Post

   Published

   Sept. 12, 2019, 1 p.m.

   ...populations with intersectional approaches is a growing field that we aim to support by growing our inventory ...

2. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

   Type

   Blog Post

   Published

   Sept. 3, 2019, 1:15 p.m.

   ...when studying neuronal activity. The researchers identified MerMAIDS from their analysis of different publicly...

3. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

   Type

   Blog Post

   Published

   March 24, 2016, 2:30 p.m.

   ... genome editing in human cells via delivery of purified Cas9 ribonucleoproteins." Genome research 24.6...

4. Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis

   Type

   Blog Post

   Published

   Aug. 28, 2014, 3:34 p.m.

   ... have the full plasmid sequence. Once you have purified plasmid DNA, this method can be done right in ...

5. GCE4All: Making Genetic Code Expansion Accessible

   Type

   Blog Post

   Published

   June 15, 2023, 1:15 p.m.

   ...that wasn’t enough, in this development-focused field, it’s not uncommon to find protocols that require...

6. Synthesized by Ginkgo Bioworks, Shared by Addgene: SARS-CoV-2 Plasmids for Many Expression Systems

   Type

   Blog Post

   Published

   May 19, 2020, 1:15 p.m.

   ...and meet our QC standards. If any concerns are identified, we can quickly put a hold on all orders and ...

7. Plasmids 101: Inducible Promoters

   Type

   Blog Post

   Published

   Jan. 18, 2018, 2:34 p.m.

   ...promoter and ceases repression of the target gene. A simplified lac inducible system is found in many bacterial...

8. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...mismatch assays require between 200 and 500 ng of purified PCR product. Low detection limits: Digital PCR...

9. Addgene’s Viral Service - Why Virus? Why Now?

   Type

   Blog Post

   Published

   March 23, 2017, 2:30 p.m.

   ...plasmids in a ready-to-use viral format. Having identified this need in the community, we approached it ...

10. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ...cell type. The human synapsin promoter further specifies neuron-specific expression of the DREADD (Kügler...

11. AAV Q&A with Tim Miles

    Type

    Blog Post

    Published

    May 17, 2022, 1:15 p.m.

    ...allow AAV re-administration but I do not think the field has settled on ‘best practices’ for model organisms...

12. Antibodies 101: ChIP

    Type

    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...analysis via any methods suitable for isolated and purified DNA, such as sequencing or qPCR. Yay!  Where’s...

13. Sweating the Small Stuff: Details in the Lab

    Type

    Blog Post

    Published

    Feb. 8, 2022, 2:15 p.m.

    ...to Try  Checklists Found in surgical suites, air fields, and military bases, checklists are the ultimate...

14. Antibodies 101: Buffers, Storage, and Conjugates

    Type

    Blog Post

    Published

    Feb. 1, 2022, 2:15 p.m.

    ...introduction to the wide, wild, and fascinating field of signaling molecules. Assay Type Westerns and ...

15. Antibodies 101: Monoclonal Antibodies

    Type

    Blog Post

    Published

    June 29, 2021, 1:15 p.m.

    ...produced by the hybridoma. Any genetic drive can be identified by sequencing the heavy and light chains. To ...

16. Targeted Mutagenesis with EvolvR

    Type

    Blog Post

    Published

    Feb. 21, 2019, 1:15 p.m.

    ...resistance mutations, several new mutations were identified by targeting EvolvR to five dispersed regions...

17. How Addgene Creates Content

    Type

    Blog Post

    Published

    Nov. 17, 2022, 2:15 p.m.

    ...resources with our antibody and content team, we identified what applications should be included and what...

18. Plasmids 101: Methylation and Restriction Enzymes

    Type

    Blog Post

    Published

    June 30, 2016, 2:30 p.m.

    ... a pool of mutated plasmids which can later be verified by Sanger sequencing. How can I tell if my enzyme...

19. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...nucleotides to the 5’ spacers. The 3’ ends were modified slightly with either a 1-7 base poly(U)tail in...

20. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ...together, thus repairing the break. This is a simplified, streamlined version of this pathway and does...