We narrowed to 553 results for: PPL

1. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ...labs, BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today...

2. Multiplex Genome Editing with CRISPR-Cpf1

   Type

   Blog Post

   Published

   May 9, 2017, 2:12 p.m.

   ... a simple and effective method with multiple applications (in vitro, in vivo, transfection, viral transduction...

3. Screening for Successful Genome Editing with Digital PCR

   Type

   Blog Post

   Published

   March 30, 2017, 2:30 p.m.

   ...a gene of interest is one of the most common applications of precision genome editing, the remainder of...

4. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...cleaved by Cas9 and repaired via HDR using the supplied donor plasmid will express GFP. If there’s GFP...

5. Viral Vectors 101: Inverted Terminal Repeats

   Type

   Blog Post

   Published

   Oct. 19, 2023, 1:15 p.m.

   ... of research is important for downstream AAV applications.  Tips & FAQs Due to their high GC content and...

6. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...antibody and content team, we identified what applications should be included and what basic information...

7. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ...require there to be a culture condition that can apply enough pressure to select for variants of interest...

8. Antibodies 101: Isotypes

   Type

   Blog Post

   Published

   Oct. 19, 2021, 1:15 p.m.

   ... Isotype controls are used in antibody-based applications including flow cytometry, immunohistochemistry...

9. Viral Vectors 101: Gamma-Retroviral Packaging Systems

   Type

   Blog Post

   Published

   Aug. 6, 2024, 1:15 p.m.

   ... above (envelope, packaging, and transfer) are supplied by three types of plasmids, which are cotransfected...

10. Antibodies 101: Flow Cytometry Controls

    Type

    Blog Post

    Published

    June 6, 2024, 1:50 p.m.

    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...

11. Sharing is Caring: But How to Distribute Open Hardware?

    Type

    Blog Post

    Published

    Feb. 28, 2023, 2:15 p.m.

    ...Challenges such as the provision of an accessible supply chain for parts that are listed in the bill of ...

12. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

    Type

    Blog Post

    Published

    March 17, 2020, 1:32 p.m.

    ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...

13. Developing a cold tolerant E. coli using specialized chaperones

    Type

    Blog Post

    Published

    Jan. 28, 2020, 2:35 p.m.

    ...Additionally, we’re also working towards widening the applicability of our strategy using the pMBP-LbCas12a and ...

14. 15 Years of Addgene: The Top 15 Plasmids

    Type

    Blog Post

    Published

    Jan. 8, 2019, 1:54 p.m.

    ...cell types so it is useful in a wide range of applications. This plasmid was the top requested plasmid ...

15. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type

    Blog Post

    Published

    Dec. 6, 2018, 1:34 p.m.

    ...directed evolution to create APEX2. They have now applied the same directed evolution technique to the biotin...

16. Why and how to start studying chromatin (with Addgene's help)

    Type

    Blog Post

    Published

    June 12, 2018, 11:40 a.m.

    ...repair, or replication, or any number of CRISPR applications and thought “OK, but how does this work in the...

17. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

    Type

    Blog Post

    Published

    May 3, 2018, 1:48 p.m.

    ...proof-of-concept for pre-clinical and clinical applications of dCasRx. The identification of Type VI Cas13d...

18. Plasmids 101: Inducible Promoters

    Type

    Blog Post

    Published

    Jan. 18, 2018, 2:34 p.m.

    ... stimulate AraC binding to I1 and I2 sites. Supplementing cell growth media with glucose decreases cAMP...

19. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ...

20. How to Keep a Lab Notebook for Bioinformatic Analyses

    Type

    Blog Post

    Published

    June 2, 2016, 2:30 p.m.

    ...in my own research. Again, some of this may not apply or may not work for you. The best way to find a ...