We narrowed to 610 results for: REV

1. Hot Plasmids - October 2022

   Type

   Blog Post

   Published

   Oct. 6, 2022, 1:15 p.m.

   ...with mouse cortical neurons, far out-performing previous attempts to engineer potassium-selective channelrhodopsins...

2. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ... scientists Mollie Schubert and Shuqi Yan for reviewing this blog post. Additional Resources on the Addgene...

3. An Addgene Summer Internship

   Type

   Blog Post

   Published

   Aug. 8, 2024, 7:22 p.m.

   ...Syntaxin3, and osa-miR528. My manager, Katherine N., reviewed the list and followed up with her manager. They...

4. Embracing Serendipity: A Crucial Element in the PhD Journey

   Type

   Blog Post

   Published

   April 25, 2024, 1:15 p.m.

   ... key to unlocking new perspectives or even revolutionizing your research. Why serendipity matters Serendipity...

5. Plasmids 101: Gene, enhancer, and promoter trapping

   Type

   Blog Post

   Published

   May 2, 2023, 1 p.m.

   ...identification methods missing something? Here we will review some time-tested gene identification systems that...

6. Tips for Improving Your Next Manuscript

   Type

   Blog Post

   Published

   Nov. 22, 2016, 3:30 p.m.

   ... Blog Resources on Addgene.org Check out Our Revamped Protocol Pages to Jumpstart Your Next Experiment...

7. FastCloning: A Newer, Simpler Cloning Technique

   Type

   Blog Post

   Published

   Nov. 1, 2016, 2:30 p.m.

   ... the 3’ end of the multi-cloning site, and the reverse primer is located in the 5’ end of the multi-cloning...

8. Management for Scientists: Managing vs. Leading

   Type

   Blog Post

   Published

   March 31, 2015, 4:08 p.m.

   ... Goldsmith, with Mark Reiter Harvard Business Review 10 Must Reads: On Leadership (Articles on Leadership...

9. Advice for Choosing a Research Project

   Type

   Blog Post

   Published

   April 17, 2014, 2:45 p.m.

   ...helpful advice from the successful PIs who were previously featured in "9 Tips to Achieve Success in Academia...

10. Plasmids 101: Antibiotic Resistance Genes

    Type

    Blog Post

    Published

    Jan. 30, 2014, 3:29 p.m.

    ...directly kill bacteria (bactericidal) or slow growth/prevent cell division (bacteriostatic); however, the distinction...

11. Choosing a Good Mentor for Scientists

    Type

    Blog Post

    Published

    Jan. 16, 2014, 2:56 p.m.

    ...read more about what makes a good mentor, see the previous post in this series. Check out Joanne's Reddit...

12. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type

    Blog Post

    Published

    Aug. 11, 2020, 1:15 p.m.

    ...complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best to propagate AAV constructs...

13. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ... Stasevich lab tested several scFvs scaffolds previously proven to fold and function in living cells. ...

14. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    Published

    Sept. 5, 2019, 1:06 p.m.

    ...run that last additional experiment requested by Reviewer #3. But why isn’t the plasmid here yet? A quick...

15. Single-cell tracking of lineage and identity with CellTag

    Type

    Blog Post

    Published

    May 9, 2019, 1:15 p.m.

    ...ended up taking the ‘dead-end’ path. The CellTags revealed that cells with common ancestors tended to have...

16. Hot Plasmids and Viral Preps - September 2021

    Type

    Blog Post

    Published

    Sept. 21, 2021, 1:15 p.m.

    ...antibiotics. Simon Moore and Paul Freemont’s lab previously developed such a system in Streptomyces, a prokaryotic...

17. Antibody Neutralization Response Against Pseudoviruses Expressing SARS-CoV-2 Spike Protein Variants

    Type

    Blog Post

    Published

    June 22, 2021, 1:15 p.m.

    ...studies immunity and the host-pathogen interface, previously developed a high-throughput neutralization assay...

18. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

    Type

    Blog Post

    Published

    May 25, 2021, 2:29 p.m.

    ...expression of the mAb-encoding genes, die, or fail to revive after cryopreservation (Bradbury & Plückthun, 2015a...

19. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type

    Blog Post

    Published

    June 2, 2020, 1:15 p.m.

    ... and structural deformation, these probes are reversible and do not cause structural deformation or photo-induced...

20. Meet the New Addgene Board Members!

    Type

    Blog Post

    Published

    April 28, 2020, 1:14 p.m.

    ...biggest potential to transform patients' lives. Previously Stegmeier was the Head of Oncology Target Discovery...