We narrowed to 524 results for: SHE
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TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~...
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Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....Addgene blogpost and more recently in a paper published in F1000Research on November 2016. Due to the ... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....and therefore each target protein. This can be accomplished by using a panel of primary antibodies conjugated... -
Viral Vectors 101: An Introduction to AAV
TypeBlog PostPublishedMay 30, 2023, 1:15 p.m....Serotype refers to the type of capsid (outer protein shell) used to package an rAAV vector; these capsids allow... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....Your video conferencing software freezes and then crashes. You’re muted on Zoom again. These things don’... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m.... PI or corresponding author of the paper it’s published in. That’s also a great way to get to know people... -
Antibodies 101: Conjugation
TypeBlog PostPublishedMarch 26, 2024, 1:15 p.m....conjugated antibodies that are ready to use off the shelf. So why are we talking about this? Even though there... -
To Each HIS Own
TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....used for a variety of assays in the lab (for a refresher see Antibodies 101: Introduction to Antibodies... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m.... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m.... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....as a society are to figure out the best way to shepherd technologies through thorny ethical issues, ensure... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....would be much easier with a Tube Writer, which air brushes the information onto the vials instead of requiring... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....strategy that uses genetically engineered mice. Best wishes on your experiment! References Becher, Burkhard... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....wells while the protein-antigen complexes will be washed away during the protocol. The amount of antibody... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....Originally published May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....lunch on your schedule, but it will probably be rushed and you’ll have to answer questions between bites...