We narrowed to 598 results for: abo.1

...depends on what you are looking to study. Figure 1: A schematic showing the differences in chemogenetics...Additional resources on the Addgene blog Read more about optogenetics New to viral vectors? Browse our Viral...Viral Vectors 101 blog posts Learn more about precise targeted manipulation Resources on Addgene.org ...

...proteins, DNA, and RNA used in CRISPR.   Figure 1: Overview of the parts of CRISPR. The bacterial chromosome...used to target complementary DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA...itself, simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer ...

...datasets by computational biologists. Figure 1: The BioGRID Open Repository of CRISPR Screens (ORCS...resources on the Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our...on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for...

...et al., 2019; McLaughlin, 2019).    Figure 1: Schematic of multiplex immunofluorescence approaches...). Springer New York. https://doi.org/10.1007/978-1-4939-8935-5_26 Jambor, H., Antonietti, A., Alicea,...to label the rat brain sample shown in Figure 2A above, Andrews et al. used the following combination of...

... antibody recognizes and binds to.   Figure 1: Labeled diagram of an antibody including Fc, Fab,...45. https://doi.org/10.1016/B978-012198382-6/50026-1 Wang R, Xiang S, Feng Y, Srinivas S, Zhang Y, Lin ...is referred to as an antigen. You can learn more about antibodies in our Antibodies 101 animation, What... expressing this protein. Read on to learn more about antibodies and how to use them in the lab! Parts...lots is a big concern. You can find more details about polyclonal antibodies on our Antibodies 101: Polyclonal...not necessarily what you are getting. Learn more about monoclonal antibodies here. Recombinant antibodies... as well as compare between samples. Learn more about flow cytometry. Cell and tissue labeling Antibodies...

...you do just this, probably in less than a minute (1). Green Listed is a new software tool used to design...found through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries Browse All...

...plasmids. The Zhang lab, on the other hand, uses a 1 plasmid, single infection system. Genome wide mouse...Biotechnology. 2013 Dec 23. (Article). Read more about CRISPRs: Using CRISPR/Cas to Edit Disease Out of...

...save that application for another day! References 1. Tanenbaum, Marvin E., et al. “A protein-tagging system...Avoid Fluorescent Protein Oligomerization Learn about Small Molecule Fluorphores Resources on Addgene.org...

...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...everything now is about the plot. This part is easy, after all, who working in a laboratory has never faced...dysregulated water bath boiling your experiments, just about anything can disrupt your bench work and sometimes...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...can learn with each other through globalized, collaborative thinking. LEGO and science: The potential is...while opening a LEGO set with my husband, I thought about how the limitless design possibilities of the LEGOs...

...share your science 1)   If you don’t already make it a habit to talk – really talk – about the research you...take on collaborative roles in academia, and that means it’s important to know how to talk about science...talking about and sharing some science on the one you already have. Read my earlier post about Scientists...important for scientists to get out there and talk about their work. To tell others why that work is cool... than ever before for scientists to communicate about their work and their interest in science more generally... the details of your science but also something about how science really works. 2)   Read science writing... told. Twitter can be a good way to follow news about particular subjects or find out what others are ...

...in online patient/caregiver communities  Table 1: Examples of defining your audience for science communication...our Summer SciComm Series, where we’ll be talking about science communication, best practices in the field...research.   This summer, we’re making time to talk about my favorite thing - science communication (scicomm...endless.    Activity Example audience  Talking about your career as a scientist underrepresented minorities...students in AP biology classes Creating a podcast about genetics using dog breeds as an example adults ...get into the more exciting parts - learning more about science communication itself! See you next time!...

... Figure 1: Antibody against PSD-95 from the Trimmer Lab. Information about the antibody, the... also be collecting feedback from the community about how the antibodies work in various applications,...antibodies are plasmid-based and recombinant. Learn more about recombinant antibodies here. Finally, Addgene is...blog and Antibody Guide, which include information about immunofluorescence, conjugates, isotypes, and more...learn more or get started.  If you have suggestions about how to make the resource better or ideas for new...

...materials to the research community. References 1. Coroadinha, A. S., et al. "Effect of osmotic pressure...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

...class.   Four ways to improve your next manuscript 1. Be consistent with your figures The figures of a ...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying... manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...

...could be kept frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box...Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...by flexibly deploying IgnaviCas9 without concern about its stability under harsh conditions. One example...

...enables the high-resolution tracking of more than 1 million cancer cells under drug treatment.” Stegmeier...science,” she says. In 11th grade she heard a talk about Z DNA that inspired her to major in molecular biology...was through this connection that she learned more about Addgene. “The mission to share critical reagents... molecular research, but also became passionate about virology,” he says. He was working in an HIV lab...gene therapy either through direct industry collaborations, grants, or setting up commercial and nonprofit...

...year and happy reading! Top 10 blog posts of 2015 1. CRISPR 101: Homology Directed Repair Addgenie Chari...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

...impartial. The best science happens when scientists have 1) the courage to make mistakes and 2) the integrity...of what I saw are that we, as a society, need to: 1) use more citations for out data so our sources are...experiments (and editors) can move slowly, news about possible broad changes to policies seems to break...primarily by wishful thinking. The March for Science is about fighting scientific partisanship, including more... could walk around and ask scientists questions about their work. Thousands of people showed up; some ...justice. Whether it’s used to fight the myths that abortions cause breast cancer and infertility or to argue...reproductive age live in a state with at least two abortion restrictions that conflict with scientific evidence...

...targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity...Chapel Hill, where he studies enhancers and blogs about science.     Additional Resources Learn how you...can use FRET to study protein interactions Learn about other types of fusion proteins Study chromatin interactions...

...the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes the passive...making, and motor control.  What do we understand about Dopamine? DA is produced by specific dopaminergic...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...