...knew that the best days in the classroom are characterized by intense student engagement and joy, rather...development comes from the Caltech lab of Professor Richard Murray. Murray’s lab uses cellular extracts to ...collaboration has resulted in a new BioBuilder book chapter and an illustrated, downloadable classroom poster...

...non-coding portion of the genome. Mendenhall and Richard Myers of HudsonAlpha (where Mendenhall is also ...transcription factor. The FLAG antibody has been well characterized and the system allows you to add this same antibody... Besides being fast and efficient, what other challenges does this system help to solve? Myers: Only a...

...contributed by David Wyatt and Dale Ramsden, UNC at Chapel Hill. One advantage to using the CRISPR/Cas system...heterogeneous. There is consequently about a two-thirds chance of causing a frameshift mutation. Of some importance...the Lineberger Comprehensive Cancer Center at UNC Chapel Hill.   References Bennardo N, Gunn A, Cheng A,...

...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing... you look unprofessional and doesn’t help your chances of getting your manuscripts published in the future...manuscript full of spelling errors will not have a great chance of getting published. In summary, it is a good...

...for genetic analysis, as well as an efficient mechanism of homologous recombination to facilitate simple...primarily pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features...required for the replication of fission yeast, Schizosaccharomyces pombe, vectors at the end.) In a previous...

.... In a recent issue of Cell Stem Cell, Musunuru, Chad Cowan and their colleagues describe a much more ...genome editing tool with which one can introduce changes into the genomes of mammalian or other cells. TALENs...for a particular sequence, there is a much better chance they will bind that sequence. That’s the revolutionary...

...abilities is that its fluorescence emission spectra changes when it goes from a more neutral pH compartment...conditions. Rosella makes it easier to study the mechanisms behind autophagy by tracking what’s being transported...17224625. 5. Noda, T., & Klionsky, D. J. (2008). Chapter 3 The Quantitative Pho8Δ60 Assay of Nonspecific...

... Tsang AAVs are a great gene therapy delivery mechanism, but their small packaging capacity of 4-5 kb ...are proteins that detect and output the voltage changes in neural activity when paired with appropriate...green-fluorescent GEVIs and blue-light-sensitive channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids...

...Technical difficulties turn your slide deck into a chalk talk. Your translator doesn’t know the term “diving... speaking is hard. Aside from the diving board charades, I doubt anyone but me remembers any of the incidents...opportunities, and gives your entire audience a chance to comfortably engage with you. If you can give...

...game for connecting to others.  Pro tip: You can change the option for sending LinkedIn messages to Click...without accidentally sending before you’ve had a chance to proof read. This might not be important for ...Turn off notifications to your network of profile changes. If you are working, and all of a sudden you start...

...is placed on the Addgene website, it sets off a chain reaction of events throughout all of Addgene - within...If something happens to the worm that requires a change of tube, there’s no clean way to separate the contents...a separate worm model and tube model, we can interchange them easily as needed. In this case, the physical...

...by the general public (either through taxes or charitable donations) so there is an increasing realization...funding or not; applications for funding from charities, commercial organizations, or universities may...-doc at Cambridge University where he studied mechanisms of cell division. Recently Seán got the entrepreneurial...

...gamma-retroviral packaging plasmids are not interchangeable. General envelope plasmids, such as VSV-G, ...interference (RNAi) is an RNA-mediated gene silencing mechanism. Scientists can express siRNAs to direct the cellular...that can reveal how genetic alterations lead to changes in phenotype. The plasmid comes with a 1.9 kb stuffer...

...longer Cas remains active in a cell, the greater chances there are for off-target edits to occur. Although...effects Prolonged Cas activity can increase the chance of off-target editing particularly after the majority...that have the gene deletion could replicate when challenged with the S. islandicus CRISPR-Cas system. 5. ...

...three species are still greater than random chance. The chances of a promoter expressing in primate and human...the same transgene: an optogenetic reporter, channelrhodopsin, fused to a fluorescent GFP marker (CatCH-...

...particularly hard. There are millions of them. The real challenge is finding relevant papers. The latest installment...publications. Bonus: papers have to be summarized in 280 characters! Using Twitter to find papers  You’ll need to...a literature discovery platform developed by the Chan Zuckerberg Initiative. Meta is not a traditional...

...think of bacteria when we think of plasmids, both Archaea and Eukaryota can also harbor these circular DNA..., Moody, T., Rice, P. A., Yu, M. K., Mimee, M., Chang, E. B., Ruscheweyh, H.-J., Sunagawa, S., Mclellan...Plasmids Isolated from Lactic Acid Bacteria:Their Characteristics and Potential Applications in Biotechnology...

...a DNA sequence ranging from a single nucleotide change to large insertions like the addition of a fluorophore...retroviral transduction efficiency by neutralizing the charge repulsion between virions and the cell surface,...pronucleus of the zygote which is both technically challenging and can negatively impact zygote viability. Nanoblades...

...NGS in 2017 for all incoming plasmids was a big change for Addgene - we had used Sanger sequencing for.... Priority number one was to ensure that this changeover had no impact on our ability to provide requesting...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

...will be greatly reduced, allowing for clearer characterization of the location, morphology, and circuit mapping...Schnütgen F, Doerflinger N, Calléja C, Wendling O, Chambon P, and Ghyselinck NB. A directional strategy for...and Sternson SM. 2008. A FLEX switch targets Channelrhodopsin-2 to multiple cell types for imaging and long-range...