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... and deep-brain fiber photometry.  Figure 1: Characterization of iGluSnFR4 in mice. A) Scheme ...CycT1 or PolII-CTD antibodies and unlabeled MS in DLD-1 cells (colorectal cancer). Image courtesy of Brianna...Glutamate-Sensing Fluorescent Reporter (iGluSnFR) designed and improved by the Loren Looger and Kaspar Podgorski...Aggarwal et al., 2025).In this latest work, the team designed and screened a large library of iGluSnFR3 mutants... HA-, and EGFP-expressing lentiviral backbones designed right here at Addgene. Here's what makes these... stable cell line creation a breeze. Modular by design: Key elements (promoter, tag, polyA site) are flanked... plasmids for flexibility and speed, so you can design, test, and innovate faster than ever. Plus, we'...

...deletions, and all possible base-to-base changes.   1. Plasmids 101: Transformation, Transduction, Bacterial...on the Addgene blog and have created and updated guides on various topics in biology over the past year...containing these fluorescent proteins at Addgene. 7. Designing Your Chalk Talk for the Academic Job Interview...

...products by their environmental impact on a scale of 1-10: the lower the score, the lower the environmental...collect in those red biohazard bags, typically is destined for a landfill. However, Triumvirate Environmental...Then, they use this material to produce various grades of plastic lumber and distribute it to industrial...

...resume that will give you a chance at an interview:  1) Have an excellent resume ready to be submitted The...experience. Most Addgene PhD level job descriptions clearly describe that the roles do not involve bench ...submission of a job application. 3) Read job descriptions carefully Be open minded and be bold. You don...qualifications to apply for a position.  A job description is usually a wish list and the hiring manager... way to a hiring manager. Learn to confidently describe your strengths in your resume and don't be shy...

...to western blotting  In a western, proteins are: (1) separated by size, (2) transferred to a membrane,...  (3) detected using antibodies.   Figure 1: Overview of the Western blot process. The sample,...protein analyses using ELISA. Peptides 72:4–15 . https://doi.org/10.1016/j.peptides.2015.04.012 Burnette WN ... Figure 2: The western blot dry apparatus includes a stack of filter paper, a copper anode/iblot ...blocking buffers, and incubation times. If the vendor includes publications that cited the antibody, consider...abundance In addition, the thickness of the band provides information about the relative abundance of the...

...five methods for extracting DNA without a kit too. 1. Early Career Researcher Toolbox: Free Online Molecular...databases, DNA sequence manipulation, and primer design. Best wishes for the new year and your research...

...2013 edition. 10 new developments in open science 1)      In February, the Office of Science and Technology...     Science’s John Bohannon published an article describing a sting operation in which he submitted a phony...

...furimazine by Promega’s Chemistry group is a good story (1). From a deep sea shrimp to a compact powerhouse of...cellular analysis products at Promega.     References 1. Hall, M.P., et al. (2012) Engineered luciferase reporter...above 600 nm. LumiFluors Schaub, F.X., et al. (3) designed two imaging probes. One consists of NLuc fused...Reporters. The LumiFluor reporter constructs were designed to be packaged into a retrovirus for infection...BRET probe for imaging. These authors set out to design a better fluorescent protein that could be co-imaged...acceptor, the more powerful the FRET signal. In well-designed FRET-based biosensors, the intensity of the FRET...

...relations, advocacy, and writing legislation (see Figure 1 for career map). The beautiful part of science policy...about science policy as a profession. Figure 1: Science policy can lead you to many paths! For a ...uncertainty of it all. Though I had spent the first few decades of my life following a very straightforward conveyor... harassment in academia or a large company who decides how they are going pursue regulatory approval for...their Local Engagement Playbook for step-by-step guides on how to engage in your local community.  Read...comprehensive resource developed by Dr. Kelly Singel which provides an overview of all available science policy fellowships...

..., or CaURA3 selection marker. Lee et al.(1) assessed many of these fluorescent tags in S. cerevisiae, ...protein products is desired, as multiple selection markers can ensure that all desired tags have been integrated...integrated. Simply design your amplification primers with the desired targeting homology—in frame, of ...These tagging vectors are based on previously described pFA6a-link vectors and include a Kan, SpHIS5, ...former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide variety...3xHA, 13xMyc, GST, or GFP). Longtine et al.(3) describe a complimentary set of plasmids for use in S. ...

...depends on what you are looking to study. Figure 1: A schematic showing the differences in chemogenetics... Roth BL (2015) DREADDs (Designer Receptors Exclusively Activated by Designer Drugs): Chemogenetic Tools...less invasive and more flexible as it relies on designer ligands that can be simply introduced by injection...lab and Takafumi Minamimoto’s lab known as deschloroclozapine (DCZ) has been developed to meet these requirements...should be carefully controlled for in experimental design.   Optogenetics Chemogenetics Timing Fast ...Find our chemogenetics and optogenetics science guides ...

...Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169. Soo, Rochelle M., et al...wall of green algae (Soo et al., 2015). There, it divides into two cells. At the same time, it sucks out ...1983). Grasshopper parasite The parasitic worm Spinochordodes tellinii‘s larvae makes its home inside grasshoppers...

...information Since the inception of Phage Directory ~1 year ago, Sacher and Zheng found that they could expand...infection, she shared that tweet with Jan Zheng, a UX designer that she knew. “We had actually met at a lindy...to possess the correct phages will test. These includes labs with a large number of different phages or...

...problem - see our recap below! Day 1: gRNA design tools: How to Design your gRNA for CRISPR Genome Editing...given our CRISPR Plasmids and Resources a fresh new design, sorting the plasmids we have available by category...capabilities. Epigenetics: CRISPR has made it possible to design programmable epigenome editing tools, and the field...cleavage in bacteria. In mammalian systems, Cas13a degrades only the specified RNA target. Bonus: It displays...

... if the name was the correct one!)    Fig. 1: HeLa cells. Image courtesy of Josef Reischig.  ...database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks to identify and describe all ...authors of the paper in which the cell line was described. Using Cellosaurus Cellosaurus is searchable by...Cellosaurus received a report that HT-55, which was described as a colon cancer cell line, is actually a rectal...

...  Figure 1: Overview of the parts of CRISPR. The bacterial chromosome encodes a tracrRNA (in some...DNA sequences. In laboratory settings, an sgRNA includes the crRNA and tracrRNA sequences in a “single-... sequences in the CRISPR array that will become guides for future targeted degradation. Before integration...biology of Cas9. Want a simpler guide to keep at your desk? We’ve got a CRISPR cheat sheet for that! Resources...

...in less than a minute (1). Green Listed is a new software tool used to design gRNAs for custom CRISPR...found through https://wermelinglab.com/.   References 1. Panda, Sudeepta Kumar, et al. "Green listed—a CRISPR... massive amount of descriptive transcriptomics data. Custom CRISPR screens designed to target differentially...Institutet, in Sweden. It can be very time consuming to design 5 guide RNAs (gRNAs) targeting each of the 1000...moment, 13 full genome SpCas9 knockout libraries designed by different academic labs, targeting mouse, human...drug targets  Another interesting approach is to design screens against known druggable targets, preferentially...library from Addgene, and then use Green Listed to design a smaller validation screen using selected gRNAs...

...that work for our experiments!)     Figure 1: The IBEX Imaging Community front page. That’s...spatial biology enthusiasts. This dataset currently includes 1049 community-validated reagents from 20 contributors... And the dataset is rich. Experimental data includes reagent type, target species, target name, host... inspired by the open-software community when designing the IBEX Knowledge-Base, seeking to create an ...

... everyone was very approachable.     Fig. 1: Examples of DNA sequencing QC work    In addition...process When I first saw the comprehensive job description for this co-op, I was immediately interested ...had stood out to me because it gave me a full description of the tasks I would be responsible for, in comparison...it relates to other teams at the company. This provides new (and old) employees with information on how...

...datasets by computational biologists. Figure 1: The BioGRID Open Repository of CRISPR Screens (ORCS...ve just discovered that your favorite gene was described in a CRISPR screen publication. You see this mentioned...This format is inherently flexible and allows the broadest possible scope of screens to be curated, for example...other than cell viability. The following video provides an in-depth walk through of how to use ORCS:   ...