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TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....cells, and has potential use for a wide range of applications in cellular biology. Van Haren J et al. Nature... quantifying multiple gene editing activities. Click here to find the ACE reporter plasmid! Martin et...
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Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....biological concepts - and you have yourself a plant publicity crisis. The Arabidopsis Biological Resource Center...Ohio State University. In her work, she focuses in public engagement in science and promoting plant science... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m....reached the level of expression needed for most applications. Figure 1: With blue light, the VVD...Ventura. BLADE activates transcription with the flick of a light switch and shuts off just as quickly.... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog PostPublishedOct. 20, 2020, 1:15 p.m....at Addgene have gone virtual! 2. Practice public speaking For almost any science career, there will... will be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving... -
Using Addgene's New Viral Service for Your Research
TypeBlog PostPublishedNov. 17, 2016, 3:30 p.m....preparations have worked well in my lab, including in applications where we have delivered them in vivo to the ...distributing new types of materials. At the time of publication we’ve already fulfilled more than 140 orders ... -
Plasmid Cloning by PCR
TypeBlog PostPublishedMarch 29, 2016, 2:30 p.m....amplify and design primers that will bind to and replicate it. The following image shows the ends of the ...your final plasmid by sanger sequencing. DNA replication by PCR has error rates that range from roughly... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....lymphocytes, too, making them a versatile tool for application in both neuroscience and immunology. Don’t be...give it a shot. “Beginners sometimes think it’s complicated, but actually it is very good,” he said. “It ... -
Scientists Map the SARS-CoV-2-Human Interaction Network
TypeBlog PostPublishedApril 9, 2020, 1:15 p.m.... we found in our map have an impact on virus replication. While we and our collaborators are starting ...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with... -
The Importance of High Titer for AAV Transductions
TypeBlog PostPublishedMarch 1, 2018, 2:11 p.m....you will have. When possible, use low-bind or siliconized tubes to store samples as it is more difficult...anterograde transsynaptic tagging: mapping corticocollicular input-defined neural pathways for defense ... -
Revamp Your Lab Meetings With Creative Virtual Collaboration
TypeBlog PostPublishedFeb. 9, 2021, 2:15 p.m....Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....funded by the public and performed by researchers who received their educations from public schools and ... -
Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping
TypeBlog PostPublishedSept. 3, 2019, 1:15 p.m....identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....transformation or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and... -
The Power Behind NGS Plasmid Validation: seqWell
TypeBlog PostPublishedApril 19, 2017, 3:25 p.m....sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents. ... -
Antibodies 101: Flow Cytometry Controls
TypeBlog PostPublishedJune 6, 2024, 1:50 p.m....ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct... -
Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors
TypeBlog PostPublishedMarch 17, 2020, 1:32 p.m.... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day. The PACE and PANCE systems use M13... -
Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification
TypeBlog PostPublishedNov. 19, 2019, 2:08 p.m.... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...which is both small and potentially less likely to elicit a strong immune response, factors that may make...