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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 816 results for: nin

Showing: 421 - 440 of 816 results

  1. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve ... results at the bench, with a special focus on cloning and plasmid-based technologies. Commentary about...

  2. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...journey to name our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent...open until Feb. 22, 2022, and we'll unveil the winning name on Feb. 24. Stay tuned!    Original Blugene...your help naming our latest mascot, an antibody joining us on our scientific adventures.  You can submit...

  3. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...precluding analyses of T cell differentiation beginning with naïve T cells. The Framework for In vivo... is compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker...Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System: Deciphering...

  4. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...researchers will add a lower case letter to the beginning of their insert abbreviation to specify what species...by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation...the Glutamine at position 295 was mutated to an Alanine, Q295A.  Mutant version: pBACKBONE-Flag-hGene(Q295A...

  5. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...there something that we can invest our time into learning and extract the most value? This is only one of...might proceed in the future. When we think of designing experiments using CRISPR, there are two major ... general opinion. I could spend a lot of time running through each tool in detail, but that is already...the end of 2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing...

  6. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...written for general audiences. So far, we are beginning to make an impact on society and science literacy... their questions and comments.  Lastly, we are planning to launch an e-magazine this year which would ...main three points of your story. Interested in learning more? Visit their website at www.theaddictivebrain.com...

  7. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...researchers - some readily found at Addgene. Shining light on transcriptional activation using dCas9...that can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR). This ...rendering the Cas9 non-functional when split but regaining functionality when the halves are reassociated...spatially controlled and reversibly activated by turning blue light on and off. As one might expect from...

  8. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...gestures are energetic.  This can take practice and training. This is important because it creates a culture...feel that giving feedback will be perceived as complaining. They may worry that it seems like "tattling"...to feedback with your actions     Use active listening skills such as the PAC Method. P = demonstrate...

  9. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...Resource sharing shortens the time needed to go from planning an experiment to performing one.  At Addgene, ...deposited CRISPR reagents, including many gRNA-containing plasmids (McDade et al, 2016). Many of the gRNAs... plasmids or from submission of a spreadsheet containing the validated gRNA sequences alone. Sequence ...

  10. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...just its biological importance. More of Daniel's stunning work can be found on his website http://www.microworldsphotography.com...with the need to conserve these unique samples, meaning that we know very little about these specimens ...competition has gained so much public interest for its stunning images that is has been featured in Time magazine...

  11. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...  Melina talked about her grandfather’s humble beginnings as an operator of a printing press. Just as her...Fan have been supporting Addgene from the very beginning and have played an integral part in its success...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

  12. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...that you could meet with learning (scientists are trained to be good at learning new things—this is a strength...often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future...

  13. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...have used fluorescent proteins to observe what’s happening inside cells for at least a couple of decades....tissues is set by the properties of hemoglobin and melanin pigments, which absorb the majority of light below..., thus, allowing higher resolution in samples containing substantial autofluorescence background.” Just...

  14. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

    Type
    Blog Post
    Published
    Feb. 16, 2021, 2:15 p.m.
    ... your job title doesn’t change. Master’s level training can prepare you to present your work at conferences...Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic v. Non-Academic...Resources on Addgene.org Check out Addgene's job openings Find eBooks from Addgene, including the Science...

  15. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...pCMV-ABE7.10 has already earned a blue flame for its adenine base editing capabilities. Epigenetics: CRISPR ...Repair mechanisms: CRISPR 101: Non-homologous End Joining and PITCHing MMEJ as an Alternative Route for Gene...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

  16. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...Find Protocols Lab Tips to Keep Your Experiments Running Smoothly and "Gnome-Proof" This is why the “Bricking...science: The potential is endless One night, while opening a LEGO set with my husband, I thought about how... laboratory. It is priceless to see our “toys” gaining life and working as scientists in a custom made...

  17. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... the beginning of the development of ARGs and have already developed a high-throughput screening method...Shapiro, M.G. (2018). Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts....

  18. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...and it wasn’t long until the field was off and running with camelid-derived single-domain antibodies (...thanks to their small size of 12–15 kDa Ease of cloning and expression from plasmids, due to only needing...amenable to recombinant expression (and because maintaining a llama facility is out of reach and unappealing...

  19. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ... us 8 short talks on every conceivable way of examining, modifying, and improving the CRISPR-Cas9 system...characterization and implementation of this system. Monday evening's talks switched gears (or should I say circuits...genetic circuitry to the downstream output. Tuesday morning's talks were also about circuitry and synthetic ...

  20. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...Managing a team is hard and most people have some learning they need to do before they can be good at it....diversity of responsibilities, which might include training or teaching others, research, and policy development...can move to a new workplace if opportunities for learning and personal growth do not exist or if they feel...
Showing: 421 - 440 of 816 results