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We narrowed to 821 results for: nin

Showing: 421 - 440 of 821 results

  1. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

    Type
    Blog Post
    Published
    Aug. 15, 2017, 1:24 p.m.
    ...enabled scientists to see how chaotic and random the scanning was and how fast T cells could jump from one antigen...neutrophils expressing LysM-GFP (green) into a draining lymph node after infection with S. aureus (red...-presenting dendritic cell subsets in the skin-draining lymph node." Proceedings of the National Academy...PMC4743831. 17. Cavanagh, Lois L., and Wolfgang Weninger. "Dendritic cell behaviour in vivo: lessons learned...

  2. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...Program (NIH-NIDA IRP), with the stated goal of learning behavioral optogenetics in order to implement ...types of constructs available, instructions for obtaining a material transfer agreement, and list of vendors... AAV construct is limited to only a few kb so attaining neuronal specificity through promoter-directed...University.  His interests include the role of learning and memory in opioid addiction and science communication...

  3. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ... editing to pooled library screening. It also has information for planning your CRISPR experiment. For...CRISPR. We’ll have a different theme for each day. Beginning today, we’ll start with a CRISPR overview and ...

  4. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...posts. Plasmids 101: Modular Cloning and Plasmids 101: Modular Cloning Applications and Kits We are... An Introduction to Gating in Flow Cytometry, Designing Your First Flow Panel, and Beyond Surface Labeling...

  5. Addgene’s New Way to Help You Share Science

    Type
    Blog Post
    Published
    Nov. 14, 2013, 4:10 p.m.
    ...repository, and explaining how to deposit and request plasmids, but I also spend a lot of time listening. I’ve ... results at the bench, with a special focus on cloning and plasmid-based technologies. Commentary about...

  6. Our New Antibody Mascot is....

    Type
    Blog Post
    Published
    Feb. 24, 2022, 2:15 p.m.
    ...journey to name our new antibody mascot, who's joining us in preparation for our upcoming affinity reagent...open until Feb. 22, 2022, and we'll unveil the winning name on Feb. 24. Stay tuned!    Original Blugene...your help naming our latest mascot, an antibody joining us on our scientific adventures.  You can submit...

  7. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ...precluding analyses of T cell differentiation beginning with naïve T cells. The Framework for In vivo... is compatible with single gene KO and pooled screening approaches; (4) encodes a Thy1.1 selectable marker...Germline Knockout in Immune Cells Genome-Wide Screening Using CRISPR Cancer and the Immune System: Deciphering...

  8. Plasmids 101: How to Name Your Plasmid in 3 Easy Steps

    Type
    Blog Post
    Published
    Feb. 13, 2014, 1 p.m.
    ...researchers will add a lower case letter to the beginning of their insert abbreviation to specify what species...by its position (number) relative to the start Methionine (Met) followed by the one letter abbreviation...the Glutamine at position 295 was mutated to an Alanine, Q295A.  Mutant version: pBACKBONE-Flag-hGene(Q295A...

  9. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...there something that we can invest our time into learning and extract the most value? This is only one of...might proceed in the future. When we think of designing experiments using CRISPR, there are two major ... general opinion. I could spend a lot of time running through each tool in detail, but that is already...the end of 2014, tools began moving away from designing exclusively for NGG PAM targets and began allowing...

  10. Science Communication for Everyone

    Type
    Blog Post
    Published
    April 16, 2019, 1:05 p.m.
    ...written for general audiences. So far, we are beginning to make an impact on society and science literacy... their questions and comments.  Lastly, we are planning to launch an e-magazine this year which would ...main three points of your story. Interested in learning more? Visit their website at www.theaddictivebrain.com...

  11. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...researchers - some readily found at Addgene. Shining light on transcriptional activation using dCas9...that can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR). This ...rendering the Cas9 non-functional when split but regaining functionality when the halves are reassociated...spatially controlled and reversibly activated by turning blue light on and off. As one might expect from...

  12. Management for Scientists: Seeking Feedback

    Type
    Blog Post
    Published
    Jan. 8, 2015, 2:34 p.m.
    ...gestures are energetic.  This can take practice and training. This is important because it creates a culture...feel that giving feedback will be perceived as complaining. They may worry that it seems like "tattling"...to feedback with your actions     Use active listening skills such as the PAC Method. P = demonstrate...

  13. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...just its biological importance. More of Daniel's stunning work can be found on his website http://www.microworldsphotography.com...with the need to conserve these unique samples, meaning that we know very little about these specimens ...competition has gained so much public interest for its stunning images that is has been featured in Time magazine...

  14. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ...  Melina talked about her grandfather’s humble beginnings as an operator of a printing press. Just as her...Fan have been supporting Addgene from the very beginning and have played an integral part in its success...helped to edit and add to this post. Interested in learning more about the last 10 years @Addgene Check out...

  15. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    Published
    July 9, 2015, 2:30 p.m.
    ...that you could meet with learning (scientists are trained to be good at learning new things—this is a strength...often is the most effective tactic a scientist in training can adopt to ensure opportunities in the future...

  16. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...have used fluorescent proteins to observe what’s happening inside cells for at least a couple of decades....tissues is set by the properties of hemoglobin and melanin pigments, which absorb the majority of light below..., thus, allowing higher resolution in samples containing substantial autofluorescence background.” Just...

  17. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ... the beginning of the development of ARGs and have already developed a high-throughput screening method...Shapiro, M.G. (2018). Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts....

  18. New CRISPR Web Resources and #12DaysofCRISPR Recap

    Type
    Blog Post
    Published
    Dec. 12, 2017, 3:13 p.m.
    ...pCMV-ABE7.10 has already earned a blue flame for its adenine base editing capabilities. Epigenetics: CRISPR ...Repair mechanisms: CRISPR 101: Non-homologous End Joining and PITCHing MMEJ as an Alternative Route for Gene...Chavez) Day 11: Pooled libraries: Genome-Wide Screening Using CRISPR/Cas9 and Plasmids 101: NGS Quality...

  19. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    Published
    Nov. 29, 2016, 3:30 p.m.
    ...Find Protocols Lab Tips to Keep Your Experiments Running Smoothly and "Gnome-Proof" This is why the “Bricking...science: The potential is endless One night, while opening a LEGO set with my husband, I thought about how... laboratory. It is priceless to see our “toys” gaining life and working as scientists in a custom made...

  20. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...and it wasn’t long until the field was off and running with camelid-derived single-domain antibodies (...thanks to their small size of 12–15 kDa Ease of cloning and expression from plasmids, due to only needing...amenable to recombinant expression (and because maintaining a llama facility is out of reach and unappealing...
Showing: 421 - 440 of 821 results