We narrowed to 695 results for: prot

...series, you'll learn about new red fluorescent proteins, AAV tools for targeting the nervous system, and... monomeric, and cyan-excitable red fluorescent protein. Listen to Our Segment on mCyRFP1 Hot Plasmid 4... - a new, bright, and monmeric red fluorescent protein.  Listen to Our Segment on mScarlet Special thanks...

...Depositing Lab pCMV-VSV-G (Empty Backbone) Envelope protein for producing lentiviral and MuLV retroviral particles...Didier Trono pVSV-G Expresses VSV-G envelope protein for pseudotyping NanoMEDIC particles Akitsu Hotta...Hotta pHCMV-RabiesG Contains Rabies virus G protein; see depositing paper for more information Miguel...

...Encodes insert(s) A plasmid with one or more genes, protein coding sequences, or other elements inserted into...backbones often contain epitope tags or fusion proteins that are appended to new inserts through cloning...gene cloned into your plasmid, any tags or fusion proteins contained in the plasmid, any mutations, and the...scientists to best utilize your plasmids (these can be protocols, GenBank files, etc). We can accept sequence files...

...significantly cheaper. When using a homemade plasmid prep protocol, it cost less than $10 to make 1000 lanes worth...ladder? You can find the Tan Lab’s DNA ladder prep protocol here and the pPSU1 and the pPSU2 plasmids are ...Use the Kit-Free Alkaline Lysis Plasmid Miniprep Protocol Learn How to Analyze Your Restriction Digests ...

... new ways to learn about science careers, lab protocols, and, of course, plasmids. Today we’re trying ...Domain Constructs Kit Hot Plasmid 4: The Yeast Prototrophy Kit from Markus Ralser's Lab -  Use this kit ...strains.  Listen to Our Segment on The Yeast Prototrophy Kit Special thanks to our sound engineer/producer...

... in using bioinformatic and proteomic tools to discover genes and proteins associated with pathogenesis... T7 polymerase, EcoRI, and various fluorescent proteins. Dr. Duedu’s plans for these plasmids have a heavy...

...from other nucleic acids (genomic DNA and RNA), proteins and chemical contaminants that have been used ...give scientists an indication of purity versus protein contaminants. The 260 and 230 nm ratios should ...for your research Brush up on molecular biology protocols using our video collection ...

...interested in our fluorescent protein background. Download the orange fluorescent protein background.         ... might want to check out Addgene’s Fluorescent Proteins blog topic to learn more about FRET and other ...

...viral trasduction (see the Zhang Lab's Nature Protocols Paper for more information). V2.0 This designation...variants, as well as links to the Zhang Lab's Nature Protocols Publication describing them here. References ...engineering using the CRISPR-Cas9 system." Nature protocols 8.11 (2013): 2281-2308. PubMed PMID: 24157548....

...come up. An MTA can resolve those questions! MTAs protect you and your institution, and almost all universities...rights – and the rights of your institution – are protected while we handle all the paperwork. Record ...Addgene is internationally recognized as being protective of both the providing institution and the receiving...

...the water. It’s thought that the worm produces proteins which affect the grasshopper’s central nervous...system: infected grasshoppers expressed different proteins in their brains compared to uninfected grasshoppers...manipulation in a grasshopper harbouring hairworm: a proteomics approach." Proceedings of the Royal Society B...

...capsid proteins that determine the AAV serotype. During transduction, the viral capsid proteins bind to... to the target cell’s surface proteins and this interaction determines the specificity and efficiency ...

...Pages Basic Molecular Biology Protocols Diagnostic Restriction Digest Protocol ... tags, origins of replication, and fluorescent proteins that are already included in Snapgene’s extensive...

...bacteria. Until recently the only available Cas9 proteins were isolated from mesophilic (medium heat loving... as Streptococcus pyogene’s SpCas9. These Cas9 proteins don’t work well at high temperatures, so to use...) database, Harrington et al discovered a Cas9 protein from the thermophilic bacteria Geobacillus stearothermophilus...

... in any way. For example, if green fluorescent protein (GFP) was the reporter gene and green cells were...be something phenotypic (such as a fluorescent protein or something species-specific) or a selectable ...what remains to be discovered in the eukaryotic proteome? Open Biol. (2019) 9:180241. DOI: https://doi....

...researchers from some labs have developed fluorescent-protein based DA sensors (Figure 2). The dLight1 dopamine...the new dLight1 sensor is a single fluorescent protein-based sensor. This sensor was engineered from a...Learn about Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research...

...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...fluorescent tags and activation of light sensing proteins. Furthermore, during the first 2 weeks of life...functions through opsins which are light sensitive proteins. Opsins used in optogenetic studies are not native...

...These kits provide in-depth, ready-to-teach lab protocols and supporting materials such as instructional...conditions, to guiding students through specific protocols, ABRC staff are available via e-mail and phone...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts Kit...

...the adenoviral helper plasmid which contains the proteins needed for the virus to replicate,  and the transfer...include a reporter like GFP, some smaller CRISPR-Cas proteins such as SaCas9, CRISPR gRNAs, or your gene of ... Find AAVs for your experiment Browse our AAV protocols ...

...including only titration of AAV particles and a protein stain to assess purity. For labs that produce a... blog post on AAV titers Browse viral vectors protocols and tips Resources on Addgene.org Find AAVs ...Learn more about Addgene's viral service Find protocols for working with virus ...