We narrowed to 521 results for: she
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TypeBlog PostPublishedMarch 2, 2023, 2:15 p.m....used for a variety of assays in the lab (for a refresher see Antibodies 101: Introduction to Antibodies...
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Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m..... Zhou, Xin X., Linlin Z. Fan, Pengpeng Li, Kang Shen, and Michael Z. Lin. "Optical control of cell signaling... -
Rosella: A Fluorescent pH-Biosensor for Studying Autophagy
TypeBlog PostPublishedApril 13, 2017, 2:30 p.m....Oligomerization Seeing Red: Simple GFP Photoconversion Light Sheet Fluorescence Microscopy Additional Resources on... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....fluorescent distances correlating with the previously established physical map. In comparing pairs of targets ~... -
Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins
TypeBlog PostPublishedMarch 19, 2019, 1:31 p.m....using blue light (Bubeck et al., 2018). They accomplished this by inserting a photo-sensitive LOV2 domain... -
Antibodies 101: Selecting the Right Antibody
TypeBlog PostPublishedMarch 15, 2022, 1:15 p.m....and therefore each target protein. This can be accomplished by using a panel of primary antibodies conjugated... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....Addgene blogpost and more recently in a paper published in F1000Research on November 2016. Due to the ... -
Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP
TypeBlog PostPublishedNov. 24, 2015, 3:30 p.m....purpose. Tang wondered if he could use previously established mouse lines expressing GFP in specific cell types... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m.... a very big way. In one of his latest papers, published in Molecular Systems Biology in November, Voigt... -
Plasmids 101: Cre-lox
TypeBlog PostPublishedJan. 13, 2015, 3:47 p.m.... I use Cre-lox? The Cre/lox system is a well-established research tool, especially in the field of mouse... -
CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9
TypeBlog PostPublishedNov. 14, 2024, 2:15 p.m....Cas9 targeting of splice sites. Nat Biotechnol. Published online November 5, 2018. doi:10.1038/nbt.4283 ... -
Plasmids 101: The Wide World of Natural Plasmids
TypeBlog PostPublishedOct. 8, 2024, 1:15 p.m.... Fogarty, E. C., Schechter, M. S., Lolans, K., Sheahan, M. L., Veseli, I., Moore, R. M., Kiefl, E., Moody... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m.... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....as a society are to figure out the best way to shepherd technologies through thorny ethical issues, ensure... -
Lab Automation at Addgene
TypeBlog PostPublishedMarch 22, 2018, 1:28 p.m....would be much easier with a Tube Writer, which air brushes the information onto the vials instead of requiring... -
Viral Vectors 101: Calcium Sensors
TypeBlog PostPublishedAug. 10, 2023, 1 p.m....on! References and Resources References Nasu Y, Shen Y, Kramer L, Campbell RE. Structure-and mechanism-guided... -
Antibodies 101: Choosing the Right Isotype
TypeBlog PostPublishedApril 6, 2023, 3 p.m....002215549804600813 Erickson, P. A., Lewis, G. P., & Fisher, S. K. (1993). Chapter 15 Postembedding Immunocytochemical... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m....designed to measure intracellular calcium and published in 1997. Since that time, numerous advances in... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....strategy that uses genetically engineered mice. Best wishes on your experiment! References Becher, Burkhard... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....wells while the protein-antigen complexes will be washed away during the protocol. The amount of antibody...