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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 718 results for: tat

Showing: 421 - 440 of 718 results

  2. What's New in CRISPR - September 2019

    Type
    Blog Post
    Published
    Sept. 10, 2019, 12:56 p.m.
    ... Using the structure of CasX at eight different states of assembly from cryo-electron microscopy, the ...activation and genome editing by fusing Cas9 to a mutated human estrogen receptor. This protein fusion stays...

  3. Working 9-5: an Addgene Co-op

    Type
    Blog Post
    Published
    June 2, 2022, 2:15 p.m.
    ...wanted to ensure applicants understood what the expectations were for the intended position. After submitting...Addgene 101” series, where each team gives a presentation on what they do and how it relates to other ...

  4. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    .... The Patriarchi Lab aimed to overcome these limitations by developing and depositing two new norepinephrine... using ColabFold (Mirdita et al., 2022). B, Representative images of HEK293T cells and neurons expressing...

  6. 9 Tips to Achieve Success in Academia

    Type
    Blog Post
    Published
    April 1, 2014, 5:33 p.m.
    ...different ways to succeed – as loner or gregarious;  dictator or laissez-faire; theory or practice; business-like...clear and attractive reports, being good at presentations and of course, networking and collaborating....

  7. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...the process, that you are not losing library representation.” It’s also important that you infect enough...selection. Shalem and Sanjana say they kept a high representation of library within cells and aimed for 300 cells...

  8. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...drop off the box at any location where the United States Postal Service picks up mail. NEB actually started...drop off the box at any location where the United States Postal Service picks up mail. Recycle plastic...

  9. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...at lower temps. This approach only works for facultative thermophiles (high OR medium heat loving), but...clears the way for CRISPR/Cas9 genome editing in facultative and obligate thermophiles without the need to...

  10. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...biosensors track cellular characteristics like pH, redox state, and voltage across membranes. These sensors generally... experimental setup? Consider what kind of instrumentation you have available, and the conditions of your...

  11. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...repaired by base excision repair, but can lead to mutations in single stranded DNA undergoing replication....endA negative, meaning the gene has been removed/mutated to neutralize the nuclease activity. For strains...

  12. Hot Plasmids - October 2022

    Type
    Blog Post
    Published
    Oct. 6, 2022, 1:15 p.m.
    ...of fast bursts, as well as slow up/down voltage states in deep tissue, in vivo. Two of their constructs...a maximum absorption of 540 nm and two-photon excitation around 1,040 nm, has larger photocurrents, and...

  13. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ... detect strigolactones (SLs) are costly, non quantitative or require heavy optimization. Claudia Vickers...fluorescence intensity, it provides a direct and quantitative detection of SLs. The authors also showed that...

  14. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...preparing for my first official professional presentation.  My first time in the lab! I got to assist...should have been nervous), but I wasn’t. The presentation went along smoothly and was followed with great...

  15. Cellosaurus: A Cell Line Information Database

    Type
    Blog Post
    Published
    Feb. 21, 2023, 2:15 p.m.
    ...depends on validated reagents along with clear documentation, so we were excited when Amos Bairoch from the... the “rabbit hole” of related cell lines when annotating a cell line Community submissions via email: ...

  16. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...each, thereby achieving the coveted “blue flame” status. We’re proud to honor the creativity, passion, ...that we cover all of the shipping logistics and facilitate all material transfer agreements, thereby freeing...

  17. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ...disease typically caused by a single R206H point mutation on the receptor ACVR1. ACVR1 triggers appropriate...regeneration in healthy patients. However, the R206H mutation makes ACVR1 responsive to members of the ubiquitous...

  18. Networking and Inspiring Young Scientists at Freshers Fairs

    Type
    Blog Post
    Published
    Nov. 17, 2015, 3:30 p.m.
    ...Society was founded in 1944 with the goal of facilitating networking between chemistry students, especially...over 170 members in its first year. The society facilitates opportunities to learn about Synthetic Biology...

  19. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...interest allowing for insertion in the correct orientation. These plasmids use a novel synthetic RPBSA promoter... exciting tool for producing specific genetic mutations and gene disruptions without the use of viral ...

  20. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...Steven Pinker. As a manager you must be able to state your observations truthfully and in clear terms....
Showing: 421 - 440 of 718 results