We narrowed to 842 results for: Eras

...piggyBac™ transposase or hyperactive transposase mutants [7], it is generally not as efficient as Cre ...How does the piggyBac™ system make it easier to generate transgenic cell lines? One of the major uses of... that have negative impacts on fitness. One consideration when making a transgenic cell line with the ...nucleases (CRISPR/Cas9, TALENs, and ZFNs). However, several challenges still remain, such as making gene edits...completely seamless (i.e., so no other genetic alterations have occurred) and isolating the specific cells...the piggyBac™ system, these challenges can be considerably reduced. The piggyBac™ transposon system can...and M. H. Wilson, “piggyBac-ing models and new therapeutic strategies.,” Trends Biotechnol., vol. 33, no...

...produced from its serum several weeks later. Created with biorender.com. To generate polyclonal antibodies... response.   Polyclonal antibodies are generated by injecting an animal with an immunogen, then...might be the full-length protein that you want to generate antibodies against, or a unique peptide sequence...Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial antibody response...designed for experiments where antibodies raised in several different species are present. One common example...antibody for your experiment, you should either order several vials of the same lot of polyclonal antibody, or...experiments to be aware of signal strength. In general, polyclonal antibodies produce a higher level of...

...ion-channels and pumps in developmental biology, regeneration studies, and cancer biology. I was first introduced...patterning. Based upon trends in studies over the past several years, it seems that the more polarized (negative...  Additionally, researchers have reprogrammed regenerating tissues without using genome engineering. Planarian... briefly inhibited in the entire worm during regeneration, the worm creates two heads. Subsequent cutting...transcriptional or translational level. However, several genetic tools have been developed to address these...exciting emerging field, with many implications for regenerative medicine, evolution of body plans, synthetic...the advancement of research in birth defects, regeneration, medicine, and synthetic bioengineering. References...

... with single-pot assembly Tracking cell-cell interactions New auxin inducible degron system for C. elegans...    New system developed to track cell-cell interactions By Jennifer Tsang GFP-based Touching Nexus (...Winslow’s lab to label cells after cell-cell interactions. Using G-baToN, “sender” cells express GFP on...anti-GFP nanobody on the cell surface. When the cells interact, GFP is transferred to the receiver cell, and ...cell pairs.   Figure 2: Schematic of the interaction between the sender cell and the receiver cell...with BFP, sender cells expressing surface BFP generated BFP-labeled receiver cells. What’s more, G-baToN...provides versibility in tracking and modulating the interactions between cells.      Tang et al., eLife 2020...

... islandicus CRISPR-Cas system. 5. Phage therapy Phage therapy uses phage to treat bacterial infections...have discovered over 50 anti-CRISPR proteins that interact with CRISPR-Cas systems such as Cascade-Cas3, ... DNA binding Example:  AcrIIA4 blocks Cas9’s interaction with the PAM site (Dong et al., 2017), and AcrIIC3...2017). Inhibit crRNA loadingExample: AcrIIC2 interactions with Cas9 interferes and prevents the correct...bacterial pathogens possess CRISPR-Cas systems, phage therapy might not be as effective. This is where Acr proteins...enough that they could be engineered to “armor” therapeutic phages to protect them from pathogenic bacteria...activity is an important step towards CRISPR-based therapies and for more precise editing in the lab. References...

... to consider one of several Cas9s that recognize NG PAMs. The Nureki lab generated SpCas9-NG, a rationally...biology, the answer is always it depends, but generally the efficiency of the desired edit is reduced ...name implies (Nishimasu et. al, 2018). xCas9 was generated by the Liu lab through phage-assisted evolution...evolution, specifically with the goal of generating Cas enzymes with alternative PAM requirements (Zhao et. ...through structure-guided engineering of the PAM-interacting domain of Cas9. After multiple rounds of engineering...helps even when it’s not an ideal site: moving several bps away from a desired site does not have the ...most gene targeting experiments. Between SpRY and several of the other Cas variants described, nearly all...

... top of the ever expanding body of biomedical literature! PubMed PubMed is the classic search engine ...Twitter into a paper-finding-machine. There are four general types of accounts you can follow: Scientists: ...updated. This Twitter list contains >300 Twitter literature bots, including a bot for biosensor papers, microbe...Semantic Scholar Semantic Scholar is an AI-backed literature search developed by the Allen Institute for Artificial...search terms. Key features Semantic Scholar has several ways to focus your search results. A bar graph ...quickly identify papers with greater influence, the average number of citations per year and the number of ...aren’t familiar with the topic.  Meta Meta is a literature discovery platform developed by the Chan Zuckerberg...

... difficult to eradicate. You can suppress the growth of mycoplasma but cannot eradicate it completely....nutrient-rich and also the best source for mycoplasma proliferation. Plus, it is added to the media after autoclaving...being contamination free. The aerosols that are generated (while talking or pipetting) during working in...leading causes of contamination. These aerosols generated will enter the culture medium during subculturing... contact with a nutrient source, they start proliferating shortly. Once they are present in the laboratory...are used which trigger cellular mechanisms that generate luminescence. This can then be detected by a luminometer...important to prevent contamination rather than eradicating it later. Don’t be scared when mycoplasma infects...

...AAAS Science and Technology Policy Fellow at the Veterans Health Administration. “But, seriously, what even...Science and Technology Policy Fellow (STPF) at the Veterans Health Administration, I could never get one straight...these changes to policy could shift how society interacts with certain scientific areas. Whether you’re ... or even run for office yourself! Plus, at the federal or state level some professional societies host...taken through your own personal lens.  For me, the Veterans Health Administration was the perfect position...example proposals and consider applying for their accelerator for some hands-on guidance.   Check out my webinar...fellowship is that you are often vetted for your general scientific expertise and then once selected, you...

... and use temperature to denature and renature the dsDNA. Upon renaturation, you will generate DNA duplexes...Expression of gRNAs Generating Mouse Models Using CRISPR/Cas9 Resources at Addgene.org Generating Stable Cell...inactivate the gene BRAF (a kinase implicated in several human cancers) in A549 cells (a human lung cancer...express Cas9. In these particular experiments, we generated A549 cells stably expressing Cas9 via transduction...52962-LV, Figure 1). Some cells were then used to generate a monoclonal cell lines by limiting dilution. ...Figure 2: Cas9 expression in monoclonal cell lines generated from A549 cells transduced with lentiCas9-blast...lanes 3, 4) were used as controls. Duplexes were generated from BRAF-edited and GFP-edited amplification ...