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...Lumio Green and Lumio Red from Invitrogen (now ThermoFisher Scientific). These reagents are nonfluorescent...is that binding is covalent, so free dye can be washed away. In addition, labeling contrast can be improved... developed, so don’t be afraid to try novel, unpublished combinations of labeling strategies. FRET experimental...

...around the same time. (The papers were actually published in the same journal on the same day.) This team...lot of terminology in this post! Here’s a quick refresher if you ever lose track of which acronym is which...10.1038/s41556-023-01342-2 Koblan, L. W., Arbab, M., Shen, M. W., Hussmann, J. A., Anzalone, A. V., Doman,...

...CRISPR editing world. The approach, originally published in Nature, is a “search-and-replace” genome editing...Yang, W., Leith, A., Daza, R. M., Martin, B., & Shendure, J. (2022). Precise genomic deletions using paired...s41587-021-00868-w Nelson, J. W., Randolph, P. B., Shen, S. P., Everette, K. A., Chen, P. J., Anzalone, ...

...optimized primers that may work better than the published ones, so if/when you use HercII or Kapa Hifi the...all FAQs from Le Cong on June 24, 2025 by Alyssa Shepard.   References and Resources References Beumer...Additional resources on the Addgene blog CRISPR Cheat Sheet Build Your CRISPR Vocabulary CRISPR 101: Off-Target...

...transcription start site. In STARR-seq, randomly sheared genomic libraries are cloned downstream of a minimal...simple and complex DNA constructs Have you ever wished you could design a gene knockdown or overexpression...characterize positive hits. This work has been published in Cell. The Moffat laboratory maintains a website...Center has kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository...

...to Cas9. All of this is accompanied by new or refreshed figures made using BioRender.com. Figure... Alyssa Shepard...

...you ever thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed...to set-up emails to alert you when papers are published on your favorite topic. If you have any other...

... serotype pAAV-hDlx-Flex-dTomato-Fishell_7 AAV2 Controls Fishell New serotype pAAV-mDlx-NLS-mRuby2...

...A newly established all-in-one vector construction system for CRISPR/Cas9-mediated multiplex genome engineering.... “However, there had been no systematically established way of making an all-in-one vector for multiplex...

...In the late 1980s, another researcher, Douglas Prasher, got the idea that this new green glowing protein...reported its sequence. Soon after that, in 1994, Prasher’s collaborator Martin Chalfie expressed GFP in exogenous...