...cell lines. The DSHB was created by the National Institute for Child Health and Human Development (NICHD)...variable, allowing exquisite specificity. This characteristic allows monoclonal antibodies to be used as ...also created the Monoclonal Antibody Research Institute dedicated to: 1) developing new ways of generating...

...loved helping to answer your plasmid and cloning questions through our Plasmids 101 series and by responding...extremely popular, generating many additional questions. We’re happy to see scientists making their own...what you’re missing. Let us know if you have any questions or if you have a topic, researcher, or technology...

...African countries. Even that grim estimate might prove to be an underestimate. Given all the excitement and...blog, where he has offered to answer readers' questions. If you have information or another resource to...

...Unfortunately, many science graduate programs are still doing an inadequate job of supporting science trainee...job of providing skills training for scientists destined to continue in academia. It seems it’s largely...postdoctoral training. A grant from the National Institute of General Medical Sciences (NIGMS) will support...

...Information" heading, you'll be directed to a page listing the articles that cite this plasmid. You can use... the "Associated Plasmids" lists Benefits to requesting scientists: Quickly locate other papers that...visibility Help us improve our citations lists We are still growing our "Articles Citing This Plasmid" database...

...researchers led by Dr. Jorge Ruas of the Karolinska Institutet, the discovery really wasn’t what they’d expected... Moon and Henriette van Praag of the National Institute on Aging in a commentary about the new work published...Martinez-Redondo V, Correia JC, Izadi M, Bhat M, Schuppe-Koistinen I, Pettersson AT, Ferreira DM, Krook A, Barres...

...subjects. Here, Sadangi and his team answer a few questions about The Addictive Brain and science communication...Addictive Brain. Our community has also requested live question and answer sessions led by scientists, which is...directly approach scientists and doctors with their questions and comments.  Lastly, we are planning to launch...

...most of the most interesting jobs are never advertised. You have to meet interesting people and maintain...skills guide Resources on Addgene.org Find job listings at Addgene Get more career insight from Addgenies...

...the entire Addgene family; the depositors and requesting scientists, all of the Addgene employees, the...research. What was once a complicated process of requesting and sharing plasmids has become simplified through...gala, one thing was clear: the sense of family is still the backbone [ba-dum-chh] of this crazy group of...

...specific cell type. Find some transduction characteristics of select AAV serotypes in Table 2 from the...set of neurons can then be targeted with optical stimuli. The restricted light exposure to a specific area...opsin expression in their cell bodies. Optical stimulation in a different brain area can then reveal the...

...scientists around the world to conduct robust investigations into the human transcriptome. The MORF library...June, we announced a new partnership with the Institute for Protein Innovation (IPI), in which Addgene...new content, a new look, and a reorganization of existing content to make it more accessible. Other accessibility...

...With many interesting articles coming out in a myriad of journals every week, it can sometimes be difficult...We do this by promoting citation, supporting sophisticated search, and curating plasmid collections. Addgene's...whether the plasmid has been used in your system. Sophisticated search You can search for specific plasmids ...

... change in response to the cell cycle or other stimuli.   To create a colorful Cas9 toolbox, Ma et al....study is the method’s sensitivity. The authors estimate that a minimum of 150-200 fluorescent protein ...epigenetics and cellular reactions to external stimuli. It could also have major applications to cancer...

...fluorescent proteins, iRFP670 and iRFP720, as photoacoustic contrast agents for two-color imaging in animals...Verkhusha says they are always looking to improve upon existing genetically-encoded probes by making them brighter...: 10.1038/ncomms3153. PubMed) Multicontrast photoacoustic in vivo imaging using near-infrared fluorescent...

...manipulate gene expression in target cells. Characteristics such as flexibility, versatility, safety, and...and straightforward as possible. If you have a question about a specific plasmid element that you would...would like answered or any topic suggestions for our Plasmids 101 series, please let us know in the comments...

...conditions, cells must be able to detect extracellular stimuli. One way they do so is through G protein-coupled...of the Rluc8 insertion within the Gα. Despite exhaustive refinement, it was not possible to generate an...a target of many pharmaceutical drugs, there is still only a partial understanding of how GPCRs signal...

...certification on social media data, and I was given a fantastic spreadsheet template to build out a report on....be useful and how to organize the metrics in a digestible manner.  At first, the numbers I presented at...smoothly and was followed with great discussion and questions. I achieved my goal of helping everyone understand...

...endogenous function of the gene as a result but will still allow for expression pattern monitoring. Enhancer...require the trapping of endogenous enhancers to stimulate the expression of the reporter. The system is ...insertion into an exon, like promoter traps, it will still likely disrupt the gene it monitors due to transcriptional...

..., or as Caitlin put it, “I get a little over-enthusiastic when it comes to the labs.” While her love ... design good lesson plans, how to design test questions, and how to design lectures. I really built a ...East Coast. When we first moved out here, I was still editing from home and looking for a job.” But Caitlin...

...The discovery of GeoCas9 started with an interesting question: why engineer a new Cas9 when you can just... GeoCas9 RNPs to be incubated at high temps but still maintain activity following heat treatment. The ...