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We narrowed to 885 results for: ANT;

Showing: 661 - 690 of 885 results

  1. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ...and shared by others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These... over 250 scientists in Europe. Despite these fantastic numbers, a lot of European scientists and institutions...

  2. In Living Color: The Skinny on In Vivo Imaging Tools

    Type
    Blog Post
    Published
    March 27, 2014, 5:14 p.m.
    ...– a liver or brain, say, or maybe a tumor. They wanted to find something better. Transparency window That...allowing higher resolution in samples containing substantial autofluorescence background.” Just last month...when in doubt, read the methods sections of the relevant papers, and then read them again. Most of the ...

  3. Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout Screening

    Type
    Blog Post
    Published
    Feb. 20, 2014, 4:57 p.m.
    ...easily manipulate specific genes. But what if you want to study genes all across the genome? Two new human... and tricks Wang offers this tip: “It's quite important, at every step of the process, that you are not...not losing library representation.” It’s also important that you infect enough target cells. What’s “enough...

  4. Fostering communication in remote collaborations

    Type
    Blog Post
    Published
    April 21, 2020, 1:14 p.m.
    ... zones, it’s important that everyone can access data and drafts of manuscripts and grant applications ...directions of the project. The state of research is constantly shifting, and long-distance collaborations are...

  5. Bringing Sustainable Practices to the Lab: Recycling

    Type
    Blog Post
    Published
    Feb. 14, 2019, 2:35 p.m.
    ...popularity of single-use items, plastic is a substantial portion of lab waste. Despite the fact that researchers...challenge you to take to action! These days, it’s important for individuals, companies, and universities to...to work on this issue. Be a champion for this important cause! To get more lab sustainability tips, check...

  6. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ... organisms: mammalian cells, yeast, drosophila, plants, worms, zebrafish, frogs, some bacteria; but not...has maximum activity from 50-70 °C. The key advantage of GeoCas9 is that it functions at temperatures...yet? Let us know in the comments below. And if you want to give GeoCas9 a try, as always, the plasmids are...

  8. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...worldwide and the presentation made it clear how important it is for us to improve our resources for this... meet hundreds of the over 11,000 conference registrants, including scientists from six continents who...presentation at the CRISPR screens symposia outlined the fantastic work this Broad Institute lab is doing creating...

  9. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...invading phages. Rising to the challenge - Evolution of anti-CRISPR genes in phages The CRISPR/Cas adaptive immune...CRISPR/Cas. Earlier this year, expression of 1 of 5 anti-CRISPR genes were found to inactivate the Type I-F... the presence of a variety of undiscovered viral anti-CRISPR genes. These data highlight an interesting...

  10. Meet the New Addgene Board Members!

    Type
    Blog Post
    Published
    April 28, 2020, 1:14 p.m.
    ...teenager in high school, I knew that this is what I wanted to do,” Stegmeier says He began this journey in...wealth of resources on Addgene’s website and blog important. “The experience from the scientific community... either through direct industry collaborations, grants, or setting up commercial and nonprofit companies...

  11. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ... of the main tenets of our quality control (QC) mantra is to do our utmost to ensure that scientists are... containing just the expression cassette that we want, it also includes a small amount of spurious DNA...packaging plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined...

  12. Summer SciComm Series: Master of SciComm

    Type
    Blog Post
    Published
    July 21, 2022, 1:15 p.m.
    ...I didn’t truly address the question of what I wanted to do when I grew up until I was all but finished... I was struck with the realisation that I didn’t want a career in neuroscience—whatever that entails. ... this subject allowed me to engage in findings relevant to my background (neuroimaging, biomechanics, ...

  13. A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light

    Type
    Blog Post
    Published
    June 17, 2021, 1:17 p.m.
    ...cultures in the shakerTurn the LEDs onAnd when you wantYou can just turn them offNo need for any washNo need... aureochrome1 (VfAu1), led to functional BLADE variants, too. The team tested different linkers between...light without the need of any cloning.     What advantages does BLADE have over AraC? When compared to the...

  14. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...evaluating CRISPR technologies, including the ever important on/off-target efficiency. Likewise, the NIST Genome... template that contains your desired edit, and I want to make sure that I (and Addgene) don’t forget this...Check out this post to see if an engineered Cas9 variant might work for your experiment. PITChing MMEJ as...

  15. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...enjoy, remember, and listen to narratives — take advantage of that in your work The part of my job I most...add author details a different person in the lab wanted to be the first author. When I raised this, he ... likely to be memorable. Work out what story you want to tell; tell it; stop. Many thanks to our guest...

  16. Viral Vectors 101: Transductions

    Type
    Blog Post
    Published
    April 30, 2024, 1:15 p.m.
    ...is the biological outcome you want to achieve? For example, do you want the nucleic acid cargo to be integrated... If you know the basics but are here because you want to know the intricacies of transductions, we've ...

  17. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...fuel sources.” In her work, Dr. Polka made R-body mutants that respond to different pH’s and some that are...School describes how AAVs function and indicates important things to consider if you’re getting started with...through Addgene.  Thanks again to all of our fantastic depositors and guest writers. Your contributions...

  18. Reagent Repositories Are Speeding up Science During the Pandemic

    Type
    Blog Post
    Published
    Aug. 4, 2020, 1:15 p.m.
    ...SARS-CoV-2 were developed for broad use and are now important for accelerating research into this virus. One...scientific community. Addgene was awarded a Fast Grant to ensure that the scientific community will continue...Deposits of new reagents, policies from journals and granting agencies requiring materials to be deposited into...

  20. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...CRISPR tools, without compromising their strategic advantages in gene editing, while keeping the system to ... array with a given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences...outperformed other tRNA candidates (either from human or plant sources) in both the strength as a promoter and ...

  21. Early Career Researcher Toolbox: Free Tools for Science Writing

    Type
    Blog Post
    Published
    April 28, 2021, 1:15 p.m.
    ...screen and transform your work into a paper or a grant. Here’s a list of tools and resources that might...differences between these two citation managers. If you want more tips on finding and organizing papers, check...post: Early Career Researcher Toolbox: Finding Relevant Papers. These are only a few of the many writing...

  22. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...be it an organic dye or FP) is one of the most important steps in the design of an imaging experiment.  ...Goedhart on the Addgene blog – outlining various important considerations when choosing a FP, but much of...valid email address).  One may also simply attach important references that serve to further characterize ...

  23. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    ...xOtH5bs4HiI Advantages of MG-2I-dL5** over other photosensitizers One of the main advantages of MG-2I-dL5... Have you ever wanted to selectively kill a subset of cells in your model system? Turns out that with...

  24. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ... worthwhile. Particularly if in the end all they want to do is to quickly generate a single cell line ...." If you ever run out of ready-to-use virus and want to make new lentiviral preps on your own, fear not...excited to provide this new service for researchers wanting an easier way to use CRISPR and would love to hear...

  25. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...be of secondary importance (or tertiary, or non-existant). On the very first night of the iPSC symposium... the stem cell community for addressing these important questions that the scientific method cannot address... it took the joint effort of five post-docs to implant human kidney progenitors into a pig. In stark contrast...

  26. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...get more done. There are also a host of other advantages to practicing effective delegation skills: ...responsibility if things go wrong. Here are some important criteria for delegating tasks appropriately: ... and accountability? What types of issues do you want to know about right away? Provide adequate support...

  27. Resume Writing for Non-academic Science Careers

    Type
    Blog Post
    Published
    Dec. 18, 2014, 4:06 p.m.
    ...understanding of the topic will give you some advantage over other candidates. Write about your transferable...research. For example, did you help put together a grant application? Are you familiar with the publishing...whether to stay in academia or not, it is even more important to start early. Think about your plan for the ...

  30. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...handle, but I wasn’t using them to my professional advantage. It wasn’t until I wrote a blog post about a research...start using social media to their professional advantage, I’ve selected five resources that have tips for...can fit into a researcher’s work day. It’s also important to remember that it’s not the amount of time you...
Showing: 661 - 690 of 885 results