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Addgene
Showing: 661 - 690 of 824 results

  1. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ... a learn a lot from these ASM pros and hope to leverage their expertise as we advance our mission of sharing...well-optimized CRISPR screening technology. Addgene got several shout-outs for making popular screening libraries...

  2. The Stingy Scientist: How the Baby Gel Box Was Born

    Type
    Blog Post
    Published
    April 15, 2014, 3:11 p.m.
    ...aliquots could be stored indefinitely at room temperature (thus also saving freezer space).  The procedure...BitesizeBio is the protocol for making your own Taq polymerase by Outreach Scientist, Andy Baltus. Addgene founder...

  3. Choosing a Good Mentor for Scientists

    Type
    Blog Post
    Published
    Jan. 16, 2014, 2:56 p.m.
    ...develop general technical proficiency, communicate your work, maintain ethical standards and interact professionally...

  4. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type
    Blog Post
    Published
    Sept. 5, 2019, 1:06 p.m.
    ...The Golden Stopwatch Award highlights TTOs that accelerate science by processing agreements quickly! If ...your institution’s TTO or legal office first. Generally, Addgene is also waiting to receive that legal...

  5. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...being targeted for autophagy. The development of several fluorescent autophagy reporters now allows for ...translation, this fusion protein is cleaved to generate one molecule of GFP-tagged LC3 for every one molecule...

  8. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    ...depositors. Below you’ll find brief summaries of several excellent guest posts from our depositors. Interested...Harvard University, describes R-bodies, “force generating protein machines that can extend and retract ...

  9. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    Published
    Aug. 11, 2020, 1:15 p.m.
    ... Repeats of Adeno-Associated Virus. Human Gene Therapy Methods 30:206–213 . https://doi.org/10.1089/hgtb...basic viral vector elements Read about four considerations for the perfect AAV tool Learn about synthetic...

  10. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ...of knowledge or interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers... have a higher level of science knowledge than average and are more likely to spend time engaging with...

  11. RUBY-Red Siliques

    Type
    Blog Post
    Published
    March 1, 2022, 2:15 p.m.
    ... plants (Arabidopsis floral dip). In the next generation the CRISPR construct needs to be segregated away...transformants can be produced. In the following generation when CRISPR needs to be removed to prevent further...

  12. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...viruses bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast... Taylor (Ed.), Viruses and Man: A History of Interactions (pp. 23–40). Springer International Publishing...

  13. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ...Plasmids 101: Cre-lox Cre recombinase can be used to generate inversions, deletions, and translocations. It ...Joining So you know that CRISPR can be used to generate knockouts, but how does it work? Guest contributors...

  14. Rewiring Metabolic Circuitry with CRISPR RNA Scaffolds [Video]

    Type
    Blog Post
    Published
    April 7, 2015, 4:21 p.m.
    ... cellular behaviors often fall short with the generation of various flux imbalances. The conventional ...authors sequentially rerouted the metabolic flux to generate differently pigmented derivatives of violacein...

  15. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ... plasmids deposited at Addgene still accrue on average 2 to 3 times as many citations as other papers ...could be growing more quickly which could also generate this type of correlation. But even with that caveat...

  16. With Our New Viral Service, We're Taking CRISPR Further!

    Type
    Blog Post
    Published
    Dec. 6, 2016, 3:30 p.m.
    ...if in the end all they want to do is to quickly generate a single cell line and proceed to use it for experiments...researcher must amplify the library, use next-generation sequencing to ensure that the library has retained...

  17. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...should recommend #PhysicalDistancing. Social interactions and #social support are even more necessary ...while. Getting started early on introductions and literature reviews could make the months leading up to your...

  18. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ...organizations in 111 countries. From 2019 to 2023, we averaged facilitating 89,477 MTAs per year.  The MTA Process...the process, from notifications and emails to generating the UBMTA link for the recipient organization...

  19. Management for Scientists: Delegating is Key

    Type
    Blog Post
    Published
    Feb. 20, 2015, 8:30 p.m.
    ...effectively is now your new measure of success. It is imperative that you resist the temptation to “just do it...of the team to ensure that no component of the overall project gets left undone or is duplicated. With...

  21. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    Published
    March 25, 2014, 3:15 p.m.
    ...creating stable cell lines and are not used for general plasmid selection. Instead, reporter genes are ...material and will subsequently pass it on to future generations. Stably transfected cells may be considered an...

  22. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...CRISPR gene editing methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) ...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...

  23. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...MOI, often resulting in a drop in transduction coverage compared to just one vector.   Q2: Do you think...a desire for very high MOI on top of very high coverage. Please see this paper for more information about...

  24. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ... of an antibody isotype? An isotype determines several key characteristics of an antibody as well as the...in their heavy chain constant regions result in several unique physical characteristics of these five isotypes...

  25. Flow Cytometry Readouts: Yes, No, and Everything in Between

    Type
    Blog Post
    Published
    Aug. 20, 2024, 1:15 p.m.
    ...(MFI) of the different populations. MFI is the average brightness from all cells that are positive for...background in protein mutagenesis and cancer immunotherapies. She is currently a Research Assistant in Arlene...

  27. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... to pursue their scientific curiosity but can literally put their work on the shoulders of giants. The... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...

  28. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then.... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

  29. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...they examined gRNA expression levels and indel generation for two PTG constructs in rice protoplasts. Compared...human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. ...

  30. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes...ade6+ purine (adenine)   S. pombe  no no   Considerations when using auxotrophic selection Of course,...
Showing: 661 - 690 of 824 results