We narrowed to 711 results for: ANG

...awhile (check out this review), but the Bruchez and Tsang labs recently developed a photosensitizer composed... in the comments below!   References 1. He, J., Wang, Y., Missinato, M. A., Onuoha, E., Perkins, L. A...

...ability to remove, reset and ligate mismatched overhangs, yielding scarless DNA assembly, and allowing ...mixture. The exonuclease removes mismatched 3’ overhangs, so that the region of homology doesn’t have to...

...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...

... Sons of Sevenless (SOS), a guanine nucleotide exchange factor that activates Ras. They expressed this...message reaches the nucleus where gene expression changes lead to cell proliferation and differentiation....

...our first blog post on choosing a Cas9, the Feng Zhang Lab had described eSpCas9, while the Keith Joung...potentially treatments. Cas12b, characterized by the Zhang (Strecker et al., 2019) and Li (Teng et al., 2018...

... of blue light, iLID undergoes a conformational change allowing high affinity binding to SspB, brings ...host organism, the expression of some genes can change depending on the location. Current methods for ...

...experimental needs, and can transduce (infect) a wide range of cell types with a high efficiency. However, gamma-retrovirus...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...

...transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral integration... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development...

...bright. Other luciferases have been modified to change the wavelength of light they emit or to optimize...been used in biosensors where BRET reports on the change in conformation of a single protein. Considerations...

...posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our...

... a function in a given process. The process can range from a defined pathway or reaction to a complex ...go fishing! References and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic...

...switching Isotype switching occurs when a B cell changes which heavy chain constant domain it pairs with...the course of an immune response since it also changes the effector function of the antibody. Isotype ...

...Morning. Karen was a founding member of Mass Medical Angels, a venture mentor at MIT and served on the Boards... Jennifer Tsang...

...Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon  You!     Chromatin-related genes...chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree ...

...Drosophila via PhiC31 Integrase-Mediated Cassette Exchange. Genetics. 173, 769–777 (2006). PubMed PMID: 16547094...16547094. PubMed Central PMCID: PMC1526508. 2. H. Jiang,  et al. Cytokine/Jak/Stat signaling mediates regeneration...

...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...

... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...

... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...

...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing...proofread your manuscript and help you correct any language- and spelling mistakes. A manuscript that is well...

... mentor met with a group of mentees to get more “bang for the buck”. Listen to our podcast interview with...years. They helped one another navigate career changes and overcome barriers by sharing broader perspectives...