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We narrowed to 709 results for: ANG

Showing: 441 - 460 of 709 results
  1. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...TRICK reporter mRNA this displacement leads to a change in the fluorescent signal: untranslated RNAs appear...experiments, it’s important to verify that these changes do not impact translation rate, as the authors ...
  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...experimental needs, and can transduce (infect) a wide range of cell types with a high efficiency. However, gamma-retrovirus...as the LTRs or structural proteins are not interchangeable between the two systems, other, more general...
  3. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...transduction in difficult-to-transfect cell types ranging from primary to embryonic stem cells. Viral integration... years ago. Since then, medical technology has changed a lot, but viruses have stuck around. Vaccine development...
  4. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...switching Isotype switching occurs when a B cell changes which heavy chain constant domain it pairs with...the course of an immune response since it also changes the effector function of the antibody. Isotype ...
  5. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...Alexander Stark Bas van Steensel Richard Young Yi Zhang Leonard Zon  You!     Chromatin-related genes...chromatin after T lymphocyte receptor signaling. Zhao K, Wang W, Rando OJ, Xue Y, Swiderek K, Kuo A, Crabtree ...
  6. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    Published
    July 22, 2025, 1:15 p.m.
    ...bright. Other luciferases have been modified to change the wavelength of light they emit or to optimize...been used in biosensors where BRET reports on the change in conformation of a single protein. Considerations...
  7. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...posts get out of date as techniques and knowledge changes and require updating or retiring. CRISPR/Cas9, ..., for example, has gone through rapid change in the past few years. Thus, we are constantly updating our...
  8. Plasmids 101: Screens vs. Selections

    Type
    Blog Post
    Published
    Nov. 10, 2022, 2:15 p.m.
    ... a function in a given process. The process can range from a defined pathway or reaction to a complex ...go fishing! References and resources References Wang, T., Wei, J.J., Sabatini, D.M., Lander, E.S. Genetic...
  9. Improved Plasmid Maps Powered by SnapGene

    Type
    Blog Post
    Published
    June 29, 2017, 1:06 p.m.
    ...easier to navigate. These practical and effective changes will give you the ability to find the plasmid with... community of scientists) for suggesting these changes, and the SnapGene and Addgene software development...
  10. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ... Nano-lantern color palette to include cyan and orange Nano-lanterns and showed that these three luminescent...eNL-based calcium sensor displaying a 500% signal change upon calcium binding. Suzuki et al. also envision...
  11. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ... is a Cas9 expression vector developed by Feng Zhang’s lab at the Broad Institute (Sanjana et al., 2014...PubMed PMID: 12595892. 4. Sanjana NE, Shalem O, Zhang F. Improved vectors and genome-wide libraries for...
  12. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...Developmental Editor at F1000Research.  With many changes taking place in the world of science publishing...proofread your manuscript and help you correct any language- and spelling mistakes. A manuscript that is well...
  13. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ... mentor met with a group of mentees to get more “bang for the buck”. Listen to our podcast interview with...years. They helped one another navigate career changes and overcome barriers by sharing broader perspectives...
  14. Kiran Musunuru on the Newest TALEN Genome-Editing System

    Type
    Blog Post
    Published
    Dec. 10, 2013, 3:13 p.m.
    ...genome editing tool with which one can introduce changes into the genomes of mammalian or other cells. TALENs...genetic backgrounds. It becomes more of an apples-to-oranges comparison. Addgene: Can you compare the TALEN ...
  15. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...This post was originally written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered...harder it is to throw in last minute experiments or changes. Fun fact: Addgene’s high-throughput approach means...
Showing: 441 - 460 of 709 results