We narrowed to 554 results for: ESP

1. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   Published

   June 26, 2018, 1:33 p.m.

   ... along with a mild or exacerbated inflammatory response which leads to nearly 25% child mortality. Since...

2. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   Blog Post

   Published

   May 22, 2018, 1:38 p.m.

   ... not all, S. pombe Met genes complement the corresponding S. cerevisiae met deficiencies (manuscript in...

3. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ...efficient oligomerization of target proteins in response to blue light. This tool, CRY2clust, was created...

4. Lab Automation at Addgene

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   Blog Post

   Published

   March 22, 2018, 1:28 p.m.

   ...helpful machines I have used in the lab. It’s especially useful if you want to avoid sore fingers. There...

5. Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors

   Type

   Blog Post

   Published

   Aug. 20, 2019, 12:31 p.m.

   ...interest may be very limited with this method, especially in adult animals, and not targeted to a specific...

6. Mouse Modeling, Part 2: Breeding and Crossing Mice

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   Blog Post

   Published

   Aug. 6, 2019, 12:55 p.m.

   ...reproduction include embryonic lethality, a short lifespan, or infertility and can arise due to copy number...

7. Fluorescent Proteins 101: Introduction to FRET

   Type

   Blog Post

   Published

   June 27, 2017, 1:03 p.m.

   ... observed light emission by fluorescence. The widespread use of FRET with fluorescent molecules, including...

8. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ...represents reality as accurately as possible. This is especially true in the case of protein tagging to elucidate...

9. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

   Type

   Blog Post

   Published

   Nov. 14, 2024, 2:15 p.m.

   ... poses the risk of disrupting adjacent genes, especially those regulated by or overlapping with the lncRNA...

10. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...are a gold mine of parts for synthetic biology, especially when combined with natural inducible expression...

11. Plasmids 101: Modular Cloning

    Type

    Blog Post

    Published

    March 12, 2024, 1:15 p.m.

    ... markers, promoters, enhancers, etc. MoClo is especially useful for more complex CRISPR experiments (e.g...

12. Hot Plasmids - October 2020

    Type

    Blog Post

    Published

    Oct. 13, 2020, 1:15 p.m.

    ...to label excitatory and inhibitory synapses, respectively. Once expressed in the neuron, the FingRs bind...

13. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    ...that have lots of general questions about job responsibilities, performance evaluations, professional development...

14. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type

    Blog Post

    Published

    June 25, 2020, 1:15 p.m.

    ...atmosphere, and the people.” My absolute favorite response to this question came from Lucy Hammer, our Financial...

15. A Look at Addgene's QC Process

    Type

    Blog Post

    Published

    May 6, 2025, 1:15 p.m.

    ... part of maintaining the quality of reagents, especially shared ones.  The importance of QC Plasmids ...

16. Hot Plasmids and Viral Preps - March 2021

    Type

    Blog Post

    Published

    March 23, 2021, 1:15 p.m.

    ...The AID system relies on a transport inhibitor response 1 (TIR1) expressed under a desired promoter with...

17. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...organisms , they have many levels of complexity, especially when trying to explore the links between genetics...

18. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type

    Blog Post

    Published

    Oct. 12, 2017, 2:26 p.m.

    ...CRISPR protein tagging does have its limitations, especially when trying to perform high-throughput experiments...

19. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    Published

    Sept. 7, 2017, 12:17 p.m.

    ... then handled. Additionally, many cell lines, especially the endocrine cell lines we most commonly work...

20. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    Published

    June 22, 2017, 2:30 p.m.

    ...commonly FPs with red and green wavelengths, respectively, are selected (6). These previous blog posts...