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We narrowed to 836 results for: Eras

Showing: 441 - 460 of 836 results

  1. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    Published
    Feb. 11, 2025, 2:15 p.m.
    ...the spotlight — Cas3. Cas3 can induce genomic alterations on a scale that other Cas effectors can’t, while...maintaining relatively high efficiency. These alterations are achieved through DNA degradation, thanks ...that the cell can use to repair. Figure 3: General overview of the Cascade-Cas3 mechanism. Created... gRNA. Cas3 appears to have fewer off-target alterations, likely due to the multi-Cas mechanism. These...has the potential to be incredibly useful in therapeutics. Imagine deleting a problematic copy of a gene...

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...light generated by sunlight to observe single gold particles with diameters less than 4nm. Lateral view...field-of-views (6). For more comprehensive reviews on several other LSFM configurations’ various features see...microscopes are less efficient than the modern cameras used in LSFM. In LSFM the laser light sheet, typically...microscopy . Since image acquisition is performed using cameras with electron multiplying couple-charged device...

  3. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...paCas9-1 using nMagC714 instead of nMagHigh1-C714, generating paCas9-2. This change did not significantly alter... alter the system’s efficiency at generating mutations when activated with light and lowered background...out the Cas9 domains in their split fusions and generate a photoactivatable nickase and a photoactivatable...protein due to being fused with high-affinity interacting proteins Coh2 and DocS, respectively. The use... the versatility of this approach. This study generated the first optically active saCas9 protein. Stop...Charpentier E, Möglich A (2013) Engineering of temperature- and light-switchable Cas9 variants. Nucleic ...

  4. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    Published
    Oct. 10, 2023, 1 p.m.
    ...formation on agar plates, less stable at high temperatures and in acidic environments.   Carbenicillin   ...mechanism. How to choose between the two? Carb is generally a ‘better’ antibiotic than amp: it is more stable...inactivates it. The NPTII gene confers resistance to several aminoglycosides, including G418 which is commonly...induce cell death by intercalating into DNA and generating DNA breaks. The Sh ble gene provides zeocin resistance...., & Brooks, G. F. (1986). Effect of storage temperature and pH on the stability of antimicrobial agents...

  5. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

    Type
    Blog Post
    Published
    Oct. 4, 2018, 12:44 p.m.
    ...Brunello library consists of 77,441 sgRNAs with an average of 4 sgRNAs per gene and 1,000 non-coding control...identifying strong hits in genetic screens, they do have several limitations. CRISPRko results in a complete loss... and the ORF overexpression library identified several overlapping genes, most genes were unique to the...Applications for the Root and Doench CRISPR libraries Overall the Root and Doench lab’s have proven their optimized..., Kendall R., et al. "Up, down, and out: next generation libraries for genome-wide CRISPRa, CRISPRi, and...

  6. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...hundreds of samples, with an average of 10 plasmids per deposit. Each week an average of 16 deposits is received...Laboratory Operations Manager at Addgene.  In addition to helping with daily laboratory operations, she also...stored right away. Every week, the lab performs an average of 140 transformations. The lab typically transforms...

  7. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ...Administrative and Operations Coordinator at Addgene. In addition to her operational responsibilities, ... was a lot of blood, sweat, and, I’m guessing, literal tears (though none of the founders have EVER confirmed...to do, feel free to crowdsource! 2. Free food generally gets people to show up If you feel like you may... event planner)! If you have a very intricate itinerary, there is a higher likelihood that something will...

  8. What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program

    Type
    Blog Post
    Published
    May 2, 2019, 12:41 p.m.
    ...they study (unless if you count normal human interaction for all of us studying human genes in test tubes...invited to attend. My job is to ensure that, in general, these labs all include materials sharing in their...Monarch butterfly food. They have chemical pathways generating interesting drug-like compounds especially cardenolides... heart disease. Caterpillars that eat milkweed generate cardenolides that tastes yucky (and is poisonous...Celina Juliano) This is a known stem cell and regeneration model. Little bits of hydra can grow back into...

  9. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    Published
    May 21, 2024, 1:30 p.m.
    ...can use your cover letter to explain why your transferable skills make you a good candidate for the position...experience or skills, often by pointing to your transferable skills. In the above example, if you don’t have...During my thesis work on [phenomenon X], I read several of your publications on [specific topic]. I am ...and grammatical errors. It does not need to be a literary masterpiece. For most jobs, the writing quality...professional. Always review grammar and spelling several times and ask someone else read your cover letter...

  10. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...their structural elements, oris interact with various RNA polymerases and proteins from bacterial hosts...ordonez, Serratia marcescens, Shigella spp., Vibrio cholerae, Xanthomonas campestris pv. campestris, X. campestris...Rhodobacter sphaeroides, Salmonella typhimurium, Vibrio cholerae, Xanthomonas campestris   In addition to incorporating... the broad host-range plasmid RK2. Molecular & General Genetics: MGG, 152(3), 129–135. https://doi.org...

  11. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    Published
    Nov. 12, 2020, 2:15 p.m.
    ... An overview of peptide synthesis. A ribosome interacts with mRNA and charged tRNAs to build a peptide...codon usage among different organisms. There are several codons that encode the same amino acid. Each organism... be harmful (Sauna and Kimchi-Sarfaty, 2011).  Overall, codon optimization may not always be the optimal...in vivo experiments and in biotechnology and therapeutics (Mauro and Chappell 2014).  Ultimately, it is...critical analysis of codon optimization in human therapeutics. Trends in Molecular Medicine 20:604–613  https...

  12. Hot Plasmids - August 2020

    Type
    Blog Post
    Published
    Aug. 18, 2020, 1:15 p.m.
    ...In their BioRxiv post, the authors generated and described several improved genetically encoded fluorescent...! Schematic of the evolution platform to generate TEV protease variants. Catalysis by TEV protease...markers can lead to non-specific labeling. They generated plasmids tagged with EB3 to mark the growing tips...plasmids containing different nanobodies (NB) were generated in pMAK:  pMAK461(NBαRabitb-IgG-Fc, Addgene #140699...

  13. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    Published
    Sept. 21, 2017, 2:07 p.m.
    .... Cas13a (previously referred to as C2c2), has several unique properties that further expand the CRISPR...other hand, uses only a short ~24 base crRNA that interacts with the Cas13a molecule through a uracil-rich...changes in the Cas13a molecule. Like Cas9, Cas13a tolerates single mismatches between the crRNA and target...interest (red) is amplified using Recombinase Polymerase Amplification (RPA) or Reverse-transcription ...knockdown to single gRNA expression. Although the collateral RNA cleavage response is well described in bacterial...

  14. Plasmids 101: Control Plasmids

    Type
    Blog Post
    Published
    April 9, 2015, 3:29 p.m.
    ...plasmid-based experiments. What are control plasmids? Generally, control plasmids help to ensure that the observed...constitutively expresses a reporter protein (e.g., GFP or luciferase) that is either co-transfected with the test ...non-targeting control also assesses any effects of the general introduction of shRNA into your target cells. Positive...replicate controls: technical and biological. In general, technical replicates can be thought of as "plate...of Plasmid A in different aliquots of cells on several different days. Biological replicates control for...

  15. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...organized pipeline and accelerated the processing of our sequencing data.”  Recently, Seqera published a case...about our bioinformatics quest, which led us to Seqera Labs, a company that provides open source workflow...You can read it, and other case studies, on the Seqera website. ...

  16. Thoughts on the Future of Research 2015 Boston Symposium

    Type
    Blog Post
    Published
    Nov. 25, 2015, 4 p.m.
    ...communicator overall. Don’t Write in a Vacuum Your research papers should go through many iterations read ...Saturday Hack Day projects sought to make an interactive game that highlights just this point. As part... this, trainees need to be able to network and interact more with people who have taken a variety of career...write for a university publication, improving your general writing skills will help you in your academic publishing...

  17. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...coupling of Cre-lox with FLP-FRT recombination to generate additional recombination events, and the synthesis...and sea anemone mKate2.) They then successfully generated custom antibodies to each of these proteins and...analysis. To achieve even cell labeling, they generated farnesylated derivatives of the fluorescent proteins...previously visible with Brainbow-1 and -2. The general structure of Brainbow-1.0 is retained in Brainbow...constructs are stably maintained through at least six generations. In addition to the neuronal pThy1-Brainbow constructs...

  18. Plasmids 101: Dimers and Multimers

    Type
    Blog Post
    Published
    Jan. 10, 2023, 2:15 p.m.
    ...twice the length of the monomer (~11.6 kb). Image generated by SnapGene software.  Thanks to increasing...which has been used in plasmid vaccine and gene therapy development to examine plasmid topologies (e.g....stationary phase. Growing the culture at a lower temperature such as 30°C or optimizing the incubation time...Fishel RA, James AA, Kolodner R. recA-independent general genetic recombination of plasmids. Nature. 1981...294184a0. PMID: 7029308. Goebel W, Helinski DR. Generation of higher multiple circular DNA forms in bacteria...

  19. Is this the right place for me? 8 tactics for choosing a lab

    Type
    Blog Post
    Published
    Oct. 2, 2018, 12:56 p.m.
    ... would thrive on this level of attention and interaction (and I did). Some of the information you will...advisor. They are always traveling, or too busy to interact regularly with the newer lab members. You must...careers?    Will you be encouraged to develop transferable skills by attending career events or being involved...your own choosing? Does the PI keep up on the literature and pass on relevant papers? Will you have access...resources on the Addgene blog Check out our transferable skills guide Learn about careers in science ...

  20. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ...started by designing a novel topology, then iteratively generated 3D models and amino acid sequences that ...topology of protein” diagrams, which show the interactions between amino acids in an approximate, two-dimensional...crosslinked peptides proposed as a scaffold for a new generation of drugs, all developed with their protein design...
Showing: 441 - 460 of 836 results