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Showing: 441 - 460 of 725 results

  1. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

  2. Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

    Type
    Blog Post
    Published
    Sept. 26, 2024, 1:15 p.m.
    ...; or by incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs...assembly is cost, as T4 polymerase is much less expensive than the enzymes required for Gibson assembly....

  3. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ...Cas enzymes and variants available. Without a comprehensive resource, it can be overwhelming to choose a...resources throughout the site. In the Experimental Considerations section on each enzyme page, you'll find a ...

  4. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...the displaced strand.   Figure 1: EvolvR consists of an error-prone polymerase (PolI) that is fused...provide a better understanding of the key residues responsible for this protein-drug interaction.   The Dueber...

  5. Early Career Researcher Toolbox: Social Media for Scientists

    Type
    Blog Post
    Published
    Dec. 11, 2018, 12:46 p.m.
    ...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

  6. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ... effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA activity...several systems to do this but most are labor-intensive, require multiple fluorescent proteins, and aren...

  8. Developing a cold tolerant E. coli using specialized chaperones

    Type
    Blog Post
    Published
    Jan. 28, 2020, 2:35 p.m.
    ...inefficiently that no industrial operation would ever consider them worth the trouble.  Enter ELSA: E. coli Learning...for research.  We’ve also involved ourselves in extensive outreach programs to encourage and embolden the...

  12. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...And there are the usual transfection drawbacks: transient expression and needing to work with a transfectable...For cloning, Zetsche et al used four oligos that consist of direct repeats and crRNA.  Similar to a jigsaw...

  14. Antibodies 101: ChIP

    Type
    Blog Post
    Published
    March 10, 2022, 3:25 p.m.
    ...is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using...exploring these interactions, and can give valuable insight into DNA expression and protein interactions, including...

  15. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...integrated into the genome of the target cell or transiently expressed. It’s important to note that for all...vector can express your construct in target cells transiently or constitutively depending on the virus used...

  16. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...monoclonal Cas9 background because you’ll have more consistent levels of Cas9 expression between experiments...pAAV-hSyn-DIO-hM4D(Gi)-mCherry Our current AAV inventory consists almost entirely of viruses expressing DREADDs ...

  17. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ... and gotten unusual or unexpected results? Or considered why DpnI will degrade your template DNA from ...level, the potential for methylation should be considered when digesting DNA. Why? Well, even though Dam...

  20. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ... transport miRNA from drug-resistant cells to sensitive ones. Though this process is not fully understood...malignancy, concluding that these cells may be considered "natural tumor suppressors." A growing interest...
Showing: 441 - 460 of 725 results