We narrowed to 740 results for: NSI

...manipulate neural populations in order to get new insights into functional network interactions. Combining...bacterial strain. The sensor will be transported inside a small 3D printed robot that will “swim” in the...

...for specific parameters: jGCaMP7s is the most sensitive responder to action potentials. For a single action... variant 1513-WPRE (105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary...

...Although highly useful, this technique requires extensive sample processing to avoid disrupting ribosome-mRNA...protein levels were negatively correlated with GFP intensity, showing that TRICK is an accurate readout of ...

...sample. The second sensor is a fusion protein consisting of sfGFP fused to a His-tagged ABC transporter... to make her work understandable to people both inside and outside the scientific community. We’re excited...

... However, the pH changes in our solutions are transient and return to normal once the tube is opened and...restored. We have no evidence to suggest that the transient change in pH affects AAV function. Furthermore...

...computer programming skills? Other things to consider are wage, opportunity to advance, and how many...jobs are normally available (as sectors such as forensics currently have too many applicants compared to...

...site of that. 3. Stem cell science is hard, and expensive Dr. Yamanaka explained that it took 50 people ...technologies, stem cell techniques are neither fast nor inexpensive.  After talking to numerous poster presenters...

...fluorescent protein fusions allowed us to image R bodies inside cells, and these constructs may be useful to future... S., Schleifer, K.-H., Verni, F. & Rosati, G. Defensive extrusive ectosymbionts of Euplotidium (Ciliophora...

...CRISPR/Cas systems have a variety of enzymes responsible for processing foreign DNA as well as the RNA...historical notes CRISPR arrays in bacterial genomes consist of repeated elements separated by unique sequences...

... effects. However, these methods can be labor intensive and quite time consuming.   As Cas9/gRNA activity...several systems to do this but most are labor-intensive, require multiple fluorescent proteins, and aren...

...Base editors make specific DNA base changes and consist of a catalytically impaired Cas protein (dCas or... to base editor activity and editing speed and consists of the following components: A plasmid in the...

...inefficiently that no industrial operation would ever consider them worth the trouble.  Enter ELSA: E. coli Learning...for research.  We’ve also involved ourselves in extensive outreach programs to encourage and embolden the...

...Cas enzymes and variants available. Without a comprehensive resource, it can be overwhelming to choose a...resources throughout the site. In the Experimental Considerations section on each enzyme page, you'll find a ...

... they flank the gene of interest. A single ITR consists of palindromic arms (A-A’, B-B’, and C-C’) that... Deng, J., Hebben, M., & Liao, J. (2024). A Comprehensive Study of the Effects by Sequence Truncation ...

...integrated into the genome of the target cell or transiently expressed. It’s important to note that for all...vector can express your construct in target cells transiently or constitutively depending on the virus used...

...is an extremely useful technique that provides insight into protein:DNA interactions. ChIP works by using...exploring these interactions, and can give valuable insight into DNA expression and protein interactions, including...

...the displaced strand.   Figure 1: EvolvR consists of an error-prone polymerase (PolI) that is fused...provide a better understanding of the key residues responsible for this protein-drug interaction.   The Dueber...

...selected to participate on this panel, but my consistent writing for the Addgene blog (~1-2 blog posts...social media, but how you use social media to consistently engage with others about your topic(s) of interest...

...; or by incomplete PCR, which skips the final extension step to retain products with single-stranded overhangs...assembly is cost, as T4 polymerase is much less expensive than the enzymes required for Gibson assembly....

...a nanobody.  AsLOV2 is a light-oxygen-voltage sensing domain from the oat plant (Avena sativa). In darkness... of more OptoNBs would allow for more uses both inside and outside cells. References Gil, Agnieszka ...