We narrowed to 553 results for: PPL

1. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

   Type

   Blog Post

   Published

   Feb. 10, 2016, 3:30 p.m.

   ...assembly methods are preferred. No such restriction applies to deletions; using REPLACR-mutagenesis, Trehan...

2. Protein Tagging with CRISPR/Cas9: A Conversation with Mendenhall and Myers

   Type

   Blog Post

   Published

   July 28, 2015, 2:30 p.m.

   ..., or words of advice for people who may want to apply this tagging system to their own work? Mendenhall...

3. Plasmids 101: Blue-white Screening

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   Blog Post

   Published

   June 4, 2015, 1:03 p.m.

   ...certain media components, and are rescued by genes supplied on the transformed plasmid. Such plasmids may ...

4. Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens

   Type

   Blog Post

   Published

   Feb. 24, 2015, 7:50 p.m.

   ...analyzing the data. It should be straightforward to apply the new library for use in various cell types and...

5. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   Published

   July 10, 2014, 7:17 p.m.

   ...competitions. After much learning, the focus of the application was changed, but Sample6 still leverages the ...

6. dTAG - You're it!

   Type

   Blog Post

   Published

   June 21, 2018, 2:06 p.m.

   ...proteins is an exciting strategy, it is difficult to apply this technique to oncoproteins that lack selective...

7. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...developed by the James and Schuh labs. Clift et al. applied TRIM-away to nine proteins in ten ddifferent cell...

8. RANbodies: Reporter Nanobody Fusions

   Type

   Blog Post

   Published

   April 10, 2018, 12:56 p.m.

   ...some reporters are better suited for particular applications. Read on to learn more about each reporter or...

9. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

   Type

   Blog Post

   Published

   March 19, 2019, 1:31 p.m.

   ...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...

10. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type

    Blog Post

    Published

    July 25, 2017, 1:20 p.m.

    ...kinases good for? There are a number of potential applications for photoswitchable kinases.  Below are a few...

11. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...were developed before CRISPR-Cas9, for use in applications like siRNA and antisense oligonucleotides. Some...

12. What's New in CRISPR - November 2021

    Type

    Blog Post

    Published

    Nov. 18, 2021, 2:15 p.m.

    ...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

13. Transferable Skills Guide: Public Speaking

    Type

    Blog Post

    Published

    April 20, 2023, 4 p.m.

    ...comes to public speaking, Murphy’s law definitely applies: what can go wrong, will go wrong. The trash removal...

14. Antibodies 101: Polyclonal Antibodies

    Type

    Blog Post

    Published

    July 14, 2021, 1:15 p.m.

    ...are particularly useful for certain research applications. Many secondary antibodies are polyclonal, as...

15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...

16. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

    Type

    Blog Post

    Published

    Dec. 8, 2016, 3:30 p.m.

    ...which can then be used for downstream cloning applications. While these two strains ultimately perform ...

17. Addgene's Tips for Plasmid Quality Control

    Type

    Blog Post

    Published

    Jan. 14, 2016, 3:30 p.m.

    ...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ...

18. The Future of Research Symposium Boston 2015

    Type

    Blog Post

    Published

    Oct. 20, 2015, 2:30 p.m.

    ...and from what fields – are needed to adequately supply these demands? Are there other solutions, such ...

19. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...proteins or co-factors, thus allowing for broad applications in a variety of experiments. LoxP (locus of ...

20. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type

    Blog Post

    Published

    July 24, 2018, 12:51 p.m.

    ...Gammaretroviral Vectors: Biology, Technology and Application. Viruses. 2011. PubMed PMID: 21994751. PubMed...