We narrowed to 526 results for: SHE
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TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m....lunch on your schedule, but it will probably be rushed and you’ll have to answer questions between bites...
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Synthetic promoter AAVs for cell-type specific expression in retinal cells
TypeBlog PostPublishedMarch 12, 2020, 1:15 p.m....work has focused on modifying the outside protein shell, or capsid, of the virus so that it only enters ... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....wells while the protein-antigen complexes will be washed away during the protocol. The amount of antibody... -
Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene
TypeBlog PostPublishedApril 6, 2021, 12:30 p.m....Originally published Aug 3, 2017 and last updated Apr 6, 2021 by Will Arnold. Introducing NGS in 2017... -
Mouse Modeling, Part 2: Breeding and Crossing Mice
TypeBlog PostPublishedAug. 6, 2019, 12:55 p.m....strategy that uses genetically engineered mice. Best wishes on your experiment! References Becher, Burkhard... -
Engaging with science and society at pgEd
TypeBlog PostPublishedSept. 20, 2018, 12:17 p.m....as a society are to figure out the best way to shepherd technologies through thorny ethical issues, ensure... -
Plasmids 101: Modular Cloning
TypeBlog PostPublishedMarch 12, 2024, 1:15 p.m.... many different MoClo kits have already been established. Keep an eye out for our next blog on choosing... -
Tips for Titering Your Lentiviral Preps
TypeBlog PostPublishedMarch 15, 2016, 2:30 p.m.... a project forward, transductions should not be rushed into. Before you start any transduction, you should... -
How-to: 5 Steps to a Great Panel Discussion
TypeBlog PostPublishedOct. 22, 2014, 1:57 p.m....This post was originally published on LinkedIn. Follow Addgene on LinkedIn for repository news and updates... -
Hot Plasmids: Summer 2025
TypeBlog PostPublishedAug. 28, 2025, 1:15 p.m....-58223-5. TnpB, so hot right now By Alyssa Shepard While CRISPR has become a household name, the search... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m.... Alyssa Shepard... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m.... Alyssa Shepard... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... Alyssa Shepard... -
Viral Vectors 101: Voltage Indicators
TypeBlog PostPublishedJan. 30, 2024, 2:15 p.m....example is attractive due to the large number of sheer molecules and signal you can generate without disturbing... -
No Llamas Required - Synthetic Nanobodies Against Membrane Proteins
TypeBlog PostPublishedJune 18, 2020, 1:15 p.m....biotinylated target protein is added and after a few washes, binding is detected using a colorimetric reaction... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m.... References and Resources References Shen C-H (2019) Amplification of Nucleic Acids. In: Diagnostic... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m.... is generally advised, there is little detail published on how to do it best. Scientists are typically... -
3D Printing Meets CRISPR Cas9
TypeBlog PostPublishedOct. 17, 2017, 12:55 p.m....could make a model of Cas9 based on the structure published by Martin Jinek’s group. We had already made an... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....fluorescently-tagged proteins at endogenous loci, shedding light on previously unknown protein functions.... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....strategy looks very similar to HDR, but it is accomplished using much smaller regions of homology, which...