We narrowed to 605 results for: abo.1

...class.   Four ways to improve your next manuscript 1. Be consistent with your figures The figures of a ...successful writer. When I originally talked to my PI about becoming a better writer, I was unsure if I should... a manuscript and critiquing it, were not too laborious, but helped build my understanding of how to generate...and the writing workshop. SWPI's writing workshop About a month later I received another email notifying... manuscript, scientists have a tendency to be elaborate and over explain the material. The point of writing...

...could be kept frozen after collection. Figure 1: Paul included a voltmeter on top of the battery box...Addgene blog Learn about other Cas proteins Read all of our CRISPR blog posts Read about GeoCas9, another... able to eliminate the need for axenic (pure) laboratory cultures to study these microbes. From 16S rRNA...by flexibly deploying IgnaviCas9 without concern about its stability under harsh conditions. One example...

...enables the high-resolution tracking of more than 1 million cancer cells under drug treatment.” Stegmeier...science,” she says. In 11th grade she heard a talk about Z DNA that inspired her to major in molecular biology...was through this connection that she learned more about Addgene. “The mission to share critical reagents... molecular research, but also became passionate about virology,” he says. He was working in an HIV lab...gene therapy either through direct industry collaborations, grants, or setting up commercial and nonprofit...

...year and happy reading! Top 10 blog posts of 2015 1. CRISPR 101: Homology Directed Repair Addgenie Chari...eyes open for any new technologies that will bring about the next revolution in biological research. We’re...Plasmids 101: Control Plasmids Need help thinking about the types of controls you’ll need for your next ...Addgene Browse Our CRISPR Resources Learn More about Viral Vectors Browse the Repository  ...

...impartial. The best science happens when scientists have 1) the courage to make mistakes and 2) the integrity...of what I saw are that we, as a society, need to: 1) use more citations for out data so our sources are...experiments (and editors) can move slowly, news about possible broad changes to policies seems to break...primarily by wishful thinking. The March for Science is about fighting scientific partisanship, including more... could walk around and ask scientists questions about their work. Thousands of people showed up; some ...justice. Whether it’s used to fight the myths that abortions cause breast cancer and infertility or to argue...reproductive age live in a state with at least two abortion restrictions that conflict with scientific evidence...

...targeting MNase to antibody bound proteins (Figure 1). Importantly, since Protein A has weaker affinity...Chapel Hill, where he studies enhancers and blogs about science.     Additional Resources Learn how you...can use FRET to study protein interactions Learn about other types of fusion proteins Study chromatin interactions...

...into three main effector functions.  Figure 1: Fc effector functions in an immune response to a ...phagocytosis.  Other functions We’re still learning about Fc effector functions and all that antibodies can...isotypes and Fc receptors that we don’t know much about yet. Who knows what kinds of functions they may ...

...the brain where DA quantitation is desired (Figure 1). The probe perfuses liquid, which causes the passive...making, and motor control.  What do we understand about Dopamine? DA is produced by specific dopaminergic...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

...the life scientist or bioengineer.   Figure 1: Map of a plasmid with its elements described below...Molecular Biology Reference includes information about molecular cloning, how to choose a plasmid vector...straightforward as possible. If you have a question about a specific plasmid element that you would like answered...

... to just that particular assay.      Fig. 1: A native protein (left) is denatured, allowing the...naturally.  Sample Denaturing  First, let’s think about induced conformation changes. Several types of assays...immunoimaging assays. It can also elucidate information about what a given protein is doing or changing in different...

...do researchers track fluorescently labeled cells? 1. Epifluorescence microscopy This technique uses a ...of the immune system and its dynamics. References 1. Peters, Nathan C., et al. "In vivo imaging reveals...fluorescence microscope where the light source is mounted above (epi) the specimen and the excitation light passes...technique that allows imaging of living tissue up to about one millimeter in depth. Two low-energy photons ...response, but the study of these dynamics was still laborious as epifluorescence microscopy wasn’t able to give...skin. Two-photon intravital microscopy. The video above depicts the infiltration of neutrophils expressing...

...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne ...know better. Networking is not about how many contacts you have; it’s about building genuine relationships...doing with nothing else to talk about. I know absolutely nothing about them and they don’t know me. It...relationships with people you care about knowing. How do you follow up with new connections and build the relationship...your new relationships Here are some ways to go about strengthening a new relationship with authenticity... on LinkedIn and always include a personal note about where you met and what you discussed. Do this in...such as FiercePharma), or sign up to be alerted about their new publications via Pubmed, so you can congratulate...

...help you decide if a master’s might be for you. 1. Are you interested in master’s-level jobs? Ultimately... Resources Resources on the Addgene blog Learn about career planning resources What Do I Do Now? Academic...

...occur, but good performers move around easily  Table 1: Taking it a Step Further - Big Company v. Small Company...students Collaborations may be less “complicated” - for example, easy to initiate a collaboration without...up the pace Constant worry about grants and renewals What is different about non-academia? Scientific...make a decision about something like this without sufficient data? Starting to learn about the diverse options...with scientists just like you What is different about academia? Publishing in peer reviewed journals ...without having to worry about proprietary information Work hours are long, but there is more flexibility with...paths to pursue. Spend some time really thinking about your motivations and what parts of being a scientist...

...approximately 20 nm of a specific DNA sequence in only 1 min. Using these lentiviral plasmids, catalytically... plasmids you need. If you'd ever like to write about a recent plasmid deposit please sign up here. Listen...

...sequencing the unique DNA barcodes. Figure 1: Schematic of barcoding with a pooled library. Created...variations in the spike protein, teaching us more about viral evolution and antibody binding. Figure...

...process is explained more fully in the animated Fig. 1 below. Normalization is a fundamental and often understated...”) of DNA, have changed our collective thinking about the feasible size and scope of projects in many ...next generation (NGS) sequencing have prevented laboratories from using these methods for routine processes...daunting for large numbers of samples. As mentioned above, one of the primary technical benefits of plexWell...1ng – 100ng) are normalized to two-fold variation above and below mean read count per sample, and the amount...NGS readout also reveals additional information about the presence of non-plasmid DNA or heterogeneous...   Check out the SeqWell website to learn more about our plexWell kits and services! Many thanks to Joe...

.... You can email her at [email protected].   References 1. Huang, Ally, et al. "BioBits™ Explorer: A modular...student at MIT. While I had always enjoyed learning about biology in high school, it wasn’t until I started...college that I really fell in love with it. Something about being able to actually hold all those seemingly ...seemingly abstract biological reactions that I learned about from textbooks just made everything click in my head...students build circuits in physics class to learn about electronics or mix chemicals together in chemistry...a power source, but now you don’t have to worry about operating the rest of the car as well. Similarly...with us! We’ll work with you to get the word out about your plasmids and their potential educational uses...

...can also rank the plasmids by abundance, scaled to 1, to create a “cumulative wealth distribution” curve... and we thought it would be a good time to talk about the amplification and verification processes we ... DNA. These reactions should allow us to obtain about 100 to 1,000 bacterial colonies per individual plasmid...from the count_spacers.py file from Feng Zhang’s laboratory. There are a number of other analysis scripts...you have any tips, input, or stories of interest about library use, let us know in the comments! Additional...NGS Sequencing to Validate AAV Preps Learn More about Pooled CRISPR Libraries Resources on Addgene.org...

...participants could share their notes together. Figure 1: Can you draw Spongebob from these instructions without... with the three other organizers. We asked them about their experiences as they navigate the new norm ...pre-workshop questionnaire to the participants to learn about what they were looking to learn in the workshop ...having a stable internet connection,” says Abitua about her workshop. “We made sure that we had a backup...on the Addgene blog Read all of our blog posts about conferences Find 25 tips for working from home ...