We narrowed to 715 results for: lic

1. Pushing the Limits of DNA Assembly

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   Blog Post

   Published

   Sept. 6, 2017, 1:01 p.m.

   ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

2. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   Blog Post

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   July 21, 2017, 12:48 p.m.

   ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and...

3. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

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   April 19, 2017, 3:25 p.m.

   ...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents.   ...

4. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

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   Blog Post

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   Sept. 3, 2019, 1:15 p.m.

   ...identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical...

5. Summer SciComm Series: A PhD in Science Communication

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   Blog Post

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   Aug. 23, 2022, 1:15 p.m.

   ...funded by the public and performed by researchers who received their educations from public schools and ...

6. Revamp Your Lab Meetings With Creative Virtual Collaboration

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   Blog Post

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   Feb. 9, 2021, 2:15 p.m.

   ...Collaboration and creativity are pivotal in writing publications/grants too. As described by several works (Wuchty...approaches can lead to frustration, delay of publication, or even the termination of a project.” Take ...

7. Fluorescent Proteins 101: Luciferases

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   Blog Post

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   July 22, 2025, 1:15 p.m.

   ...identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus), deep-sea copepod...reniformis) Natural 481 36 Coelenterazine None No Click Beetle Red (CBR)(Pyrophorus plagiophthalamus) Natural...

8. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

   Published

   March 17, 2020, 1:32 p.m.

   ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...

9. Viral Vectors 101: Biosensors

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   Blog Post

   Published

   July 6, 2023, 1 p.m.

   ...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...

10. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...light-mediated suppression of synaptic transmission in brain slices from mouse, and showed that it can alter motor ...

11. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type

    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...

12. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...

13. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post

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    Nov. 19, 2019, 2:08 p.m.

    ... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...

14. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...which is both small and potentially less likely to elicit a strong immune response, factors that may make...

15. Antibodies 101: Flow Cytometry Controls

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    Blog Post

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    June 6, 2024, 1:50 p.m.

    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...

16. Antibodies 101: ChIP

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    Blog Post

    Published

    March 10, 2022, 3:25 p.m.

    ...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give... upfront investment than many other antibody applications, this investment can well be worth it once you...

17. Antibodies 101: Fab Fragments

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    Blog Post

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    Sept. 5, 2024, 1:15 p.m.

    ... as secondary antibodies, have much broader applicability. It is fairly easy to find these in a Fab fragment...fragments described above can be used for similar applications and have similar advantages, though with some...

18. Supporting Rare Disease Research with the 2020 BeHEARD Awards

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    Blog Post

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    Sept. 8, 2020, 2 p.m.

    ...Deficiency (CTD) is a rare X-linked inherited neurometabolic condition and one of three Cerebral Creatine... into CCDS. The brain and muscles have a high metabolic demand that is powered by ATP. A molecule central...

19. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell. Intein...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...

20. Antibodies 101: Conjugation

    Type

    Blog Post

    Published

    March 26, 2024, 1:15 p.m.

    ...especially if you start to consider clinical applications). But there are a few other classes that we ...proximity ligation, immuno-PCR, and single-cell applications (Hegazy, et al., 2020; Niemeyer, et al., 2007...