We narrowed to 712 results for: lic

1. Pushing the Limits of DNA Assembly

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   Blog Post

   Published

   Sept. 6, 2017, 1:01 p.m.

   ...transforma­­­tion or as a PCR template. Some of the applications of the NEBuilder HiFi DNA Assembly Mix include...the limitations associated with otherwise more complicated assembly approaches, and opens the door for new...

2. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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   Blog Post

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   July 21, 2017, 12:48 p.m.

   ...as overexpression of each gene individually. Applications of the Gal4/UAS system There are a number of...lines. In the meantime, be sure to browse the publication references and web links from my first post and...

3. The Power Behind NGS Plasmid Validation: seqWell

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   Blog Post

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   April 19, 2017, 3:25 p.m.

   ...sequencing instruments by enhancing the efficiency and simplicity of library prep. As part of our mission, we’ve...of Toronto. He is an author of 20+ scientific publications and patents.   ...

4. Viral Vectors 101: Biosensors

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   Blog Post

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   July 6, 2023, 1 p.m.

   ...ready-to-use. While biosensors span the gamut of applications within chemistry, biology, and physics, in this...Neurotransmission and Neuromodulation: Engineering and Applications. Frontiers in Cellular Neuroscience. 2019 Oct...

5. Expanding the Targeting Scope and Editing Efficiency of Adenine Base Editors

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   Blog Post

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   March 17, 2020, 1:32 p.m.

   ... SpCas9. In order to expand the gene-editing applications and targeting scope of ABEs David Liu’s lab ...through many rounds of mutations, selection, and replications per day.  The PACE and PANCE systems use M13...

6. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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   Blog Post

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   Nov. 19, 2019, 2:08 p.m.

   ... protein purification with OptoNBs The first application for OptoNBs takes us in vitro: light-controlled...OptoNBs to control cell signaling The second application for OptoNBs takes us into cells: light-based ...

7. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

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   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...off-target activity, which may be undesirable in many applications. At the time of our first blog post on choosing...which is both small and potentially less likely to elicit a strong immune response, factors that may make...

8. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

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   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...that undergo light-induced dimerization. Upon application of blue light, iLID undergoes a conformational...light-mediated suppression of synaptic transmission in brain slices from mouse, and showed that it can alter motor ...

9. Antibodies 101: ChIP

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   Blog Post

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   March 10, 2022, 3:25 p.m.

   ...next-gen sequencing. ChIP is an incredibly useful application for exploring these interactions, and can give... upfront investment than many other antibody applications, this investment can well be worth it once you...

10. Fluorescent Proteins 101: Luciferases

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    Blog Post

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    July 22, 2025, 1:15 p.m.

    ...identified in the sea pansy (Renilla reniformis), click beetle (Pyrophorus plagiophthalamus), deep-sea copepod...reniformis) Natural 481 36 Coelenterazine None No Click Beetle Red (CBR)(Pyrophorus plagiophthalamus) Natural...

11. Antibodies 101: Flow Cytometry Controls

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    Blog Post

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    June 6, 2024, 1:50 p.m.

    ...ll need a few controls specific to flow as an application. These controls will allow you to distinguish...commercial antibody pages. Flow cytometry can be a complicated and daunting process. Controls like single color...

12. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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    Blog Post

    Published

    July 28, 2017, 1:30 p.m.

    ...throughout the lifespan of the fly to determine the implications of your genetic manipulations. You can produce...immunofluorescent images like in this paper (2). Metabolic assays such as Seahorse can be employed to look...

13. Top Requested Lentivirus and AAV of 2016

    Type

    Blog Post

    Published

    Jan. 6, 2017, 3:56 p.m.

    ... preps. Cas9 is the headliner of most CRISPR applications and can be expressed from your cell line of ... PubMed Central PMCID: PMC3069789. 3. Kügler S, Kilic E, Bähr M. Human synapsin 1 gene promoter confers...

14. All in a Twist: dsRNA

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    Blog Post

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    Feb. 2, 2023, 2:15 p.m.

    ...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...

15. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

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    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...Each half of the base editor is fused to a fast-splicing split intein and co-infected into the cell. Intein...increasingly been recognized for its biotechnological applications including conversion of CO2 into water and electrical...

16. Antibodies 101: Secondary Antibodies

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    Blog Post

    Published

    Dec. 21, 2021, 2:15 p.m.

    ...IgG2 or IgG4. This can be important for some applications. Assay use While secondary antibodies are useful..., the flexibility of their use varies across applications. Western blots, for instance, almost always ...

17. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

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    Blog Post

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    Aug. 5, 2021, 12:15 p.m.

    ... Haoyi Qiu He Tian Every two years, we seek applications for awards to early career scientists using ...virtual conference attendance. We also opened up applications to research technicians and undergraduate researchers...

18. Troubleshooting Your Plasmid Cloning Experiment

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    Blog Post

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    Sept. 24, 2019, 12:55 p.m.

    ... this stage and that can be used to simulate in silico cloning. This will definitely help in understanding...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

19. Plasmid Tools for Microbiome Studies

    Type

    Blog Post

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    June 27, 2019, 12:25 p.m.

    ...modular suite of plasmids (pGT) with various replicative origins that can be used for broad or narrow ...Central PMCID: PMC5963681.  Meyer, Richard. "Replication and conjugative mobilization of broad host-range...

20. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

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    Blog Post

    Published

    March 19, 2019, 1:31 p.m.

    ...   With the addition of blue light, the terminal helices of the LOV2 domain unfold, disrupting the protein...response to blue light (445nm). The discovery and applications of protein partners that bind to only the light...