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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 816 results for: nin

Showing: 441 - 460 of 816 results

  1. Summer SciComm: Science Storytelling

    Type
    Blog Post
    Published
    Sept. 16, 2022, 1:15 p.m.
    .... You can introduce your results right at the beginning of your communication, use conflict to get your...really dig into the significance of the results, explaining what new questions are next or how this will ...impact future research or policy.  It is worth mentioning that this is far from the only way to communication...

  2. Antibodies 101: Epitope Availability

    Type
    Blog Post
    Published
    Jan. 11, 2022, 2 p.m.
    ...abstract protein during your sample prep process, imagining how it starts and if any steps could affect its...conditions. In those cases, pay special attention to designing your controls in validation, as you may need to...help you avoid a great deal of frustration when designing and troubleshooting immunoimaging assays. It can...

  3. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ... (also referred to as TX-TL systems) have been gaining popularity as a synthetic biology tool. These systems... biosynthetic pathways. Biosynthetic pathways containing multiple enzymes could be successfully reconstituted...library targeting S. pneumoniae D39V from Jan-Willem Veening is now available with one sgRNA per operon or gene...

  5. Viral Genome Sequencing - A One Stop Shop for AAV Identity and Serotype Confirmation

    Type
    Blog Post
    Published
    April 23, 2020, 1:15 p.m.
    ...and while we think of the AAV vector genomes as containing just the expression cassette that we want, it...plasmids. We decided to use this to our advantage by designing a Python script that looks for pre-defined serotype...two types of adeno-associated virus particles containing complementary polynucleotide chains. J Virol ...

  6. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...knock-in at a genomic locus or more commonly via cloning of recombinant proteins. Why use a tag? If you...peptide (YPYDVPDYA) derived from human influenza hemagglutinin. The tag has two negatively charged amino acids...need monoclonal GFP antibodies or guidance on designing a GFP tag Addgene has you covered!   Potential...

  7. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ...Michohomology-mediated end joining (MMEJ), also known as alternative non-homologous end joining (Alt-NHEJ), has ...so much more to working with CRISPR than just designing a repair template that contains your desired edit...

  8. 10 Great Guest Posts We're Thankful For

    Type
    Blog Post
    Published
    Nov. 18, 2016, 3 p.m.
    .... Minigenomes are stripped down viral genomes containing only the sequences required for viral replication...CombiGEM and CRISPR for Combinatorial Genetic Screening CombiGEM is a technology that can be used to quickly...barcode small libraries of CRISPR gRNAs to use for screening experiments. Get the full details on how to make...

  9. Scientific Peer-review: Providing Critical and Kind Feedback and Advocating for Open Science

    Type
    Blog Post
    Published
    Aug. 8, 2019, 1:19 p.m.
    ... early peer-review attempts took place at the beginning of the 18th century. Yet the peer-review was not...first read, I am usually able to construct the beginning of my review - "The manuscript by ZZZ et al., ...do it on another day - preferably the following morning - as usually deadlines are pressing. During this...

  10. Advice for Moving Into Sales After Your Science Postdoc

    Type
    Blog Post
    Published
    Aug. 21, 2014, 3:15 p.m.
    ...after 6 years in the business of western blots and cloning, I decided to pack it in. I had just started month...Career Achievements”, and “Academic Achievements”. Leaning my CV more towards me as a product and not me as...I had no previous business, marketing or sales training and knew I had to start somewhere. I knew if I...

  11. Mentoring for Scientists: I Have a Mentor, What Now?

    Type
    Blog Post
    Published
    March 6, 2014, 7:12 p.m.
    ...– it is the discussion after that will lead to learning. Listen to our podcast interview with Harvard...this point, consider that I try to exercise every morning, but the temptation to skip the gym is strong. ...out for me that gets me out of bed and into my running shoes. Be a mentor to become a better mentee One...

  12. DAP arrays for multiplex gene editing

    Type
    Blog Post
    Published
    July 7, 2022, 1:15 p.m.
    ...potential in vivo applications or multiplex CRISPR screening.   Using tRNAs Luckily, a fun feature of tRNA...given tRNA (derived from human or plant source), by tuning the length of 5’ leader sequences of a given tRNA...engineering of crops or allow for sophisticated CRISPR screenings that would perturb the unprecedented number of...

  13. Hot Plasmids - December 2019 - CRISPR pooled library, LipoGlo, CRISPRa, and Voltage Imaging Tools

    Type
    Blog Post
    Published
    Dec. 10, 2019, 1:58 p.m.
    ... a only a single gRNA per cell. The viral DNA containing the gRNA then integrates into the genome. Thus...are poorly transduced by lentivirus, and thus screening with available CRISPR libraries was not possible...retroviral mouse CRISPR pooled library can be used for screening of cells that are refractory to lentiviral transduction...

  14. Viral Vectors 101: Inverted Terminal Repeats

    Type
    Blog Post
    Published
    Oct. 19, 2023, 1:15 p.m.
    ...Replacing the D’ region of an ITR with a sequence containing transcription factor binding sites can increase...expression at varying capacities. AAV vectors containing the AAV2 ITRs can be cross-packaged with capsids...PMID: 31914802. Savy, A., Dickx, Y., Nauwynck, L., Bonnin, D., Merten, O.-W., & Galibert, L. (2017). Impact...

  15. FPbase: A new community-editable fluorescent protein database

    Type
    Blog Post
    Published
    May 16, 2018, 1 p.m.
    ...posts by Joachim Goedhart on the Addgene blog – outlining various important considerations when choosing...contributions (using simple web forms) while also maintaining a relatively strict database design (schema)....inaccurate data submissions, so FPbase includes a versioning system that allows users to see when a given ...

  16. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...supernatant containing exo-AVVs from producer cells, and then to enrich the microvesicles containing AAVs (...Exosome-Associated AAV by Overexpression of the Tetraspanin CD9." Molecular Therapy-Methods & Clinical Development...

  17. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...fluorescent proteins and a new two-photon laser scanning microscope will allow Tsakanova to image cortical...insights into functional network interactions. Combining synthetic biology with engineering: glowing bacteria... bacterial sensor, he will construct plasmids containing promoter regions that activate fluorescent protein...

  18. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...publications from these journals into two groups: those containing plasmids that the authors deposited with Addgene... other reasons for the correlation. However, determining this exactly is difficult. For example, it is... papers in the same journals! So, is all this remaining difference caused by depositing the related plasmids...

  19. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...mass spectrometry app. I digress. When I started learning proper cell culture technique, I tried to find...my lab we do a lot of genetic construction and cloning experiments. Not all of our lab enzymes are made...be beneficial for new researchers or students beginning to learn about biochemistry. The least this app...

  20. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ...used TRICK to examine translation of Drosophila patterning gene oskar. Early in development, the osk-TRICK...with the red reporter mRNA. Yan et al. used harringtonine, a translation inhibitor that stalls ribosomes...sequences into the coding sequence and 3’ UTR. Before beginning experiments, it’s important to verify that these...
Showing: 441 - 460 of 816 results