We narrowed to 695 results for: prot

...including only titration of AAV particles and a protein stain to assess purity. For labs that produce a... blog post on AAV titers Browse viral vectors protocols and tips Resources on Addgene.org Find AAVs ...Learn more about Addgene's viral service Find protocols for working with virus ...

...goal is to provide scientists with new tools and protocols that they can easily incorporate into their own...viral particle’s affinity for heparan sulfate proteoglycans on the surface of the packaging cells (Vandenberghe...service Learn about viral production Find viral protocols here ...

...These kits provide in-depth, ready-to-teach lab protocols and supporting materials such as instructional...conditions, to guiding students through specific protocols, ABRC staff are available via e-mail and phone...Addgene.org Find Plant Vectors at Addgene Find Simple Protocols for Introductory Lab Work MoClo Plants Parts Kit...

...serotypes that can be used to deliver fluorescent proteins to cells as determined by the serotype. You can... viral vector pages as well as expanded virus protocols, information on how we produce and test virus,...Overview of Viral Production at Addgene Find Virus Protocols Browse All Viral Vectors ...

...this post. 3. Plasmids 101: E. coli Strains for Protein Production Addgenie Julian Taylor-Parker explains...might want to use a specialized E. coli strain for protein production and gives a quick rundown of some of...of the strains you can use to express your protein of interest. 4. Plasmids 101: Blue-White Screening ...

...immunoassays due to only having one binding site per protein. Having multiple antibodies allows you to use them...light over the last decade, but the fluorescent proteins (FPs) necessary for many of these applications...Campbell, B.C., et al. Chemically stable fluorescent proteins for advanced microscopy. Nat Methods 19, 1612–...

...that EvolvR could also be used to map protein-protein interactions, investigate non-coding segments of the...understanding of the key residues responsible for this protein-drug interaction.   The Dueber Lab propose that...

...Besides being compatible with any vector, the SLIC protocol itself is flexible. For one thing, those 5′ overhangs...boosts efficiency with the homologous recombination protein RecA. (It’s effective with as little as 3 ng of...Resources at Addgene Ligation-Independent Cloning Protocol Gibson Assembly Blog Post Choosing a Molecular...

...combine computational processing with biological protocols and revolutionary physical approaches. However...the structure in a similar way to the biological protocol, and assembly designs based around the use of ...commercially available tools and components. Rapid prototyping techniques such as 3D printing, on-demand CNC...

...changes and consist of a catalytically impaired Cas protein (dCas or Cas nickase) fused to a DNA-modifying .... 2). These systems allow for rapid continuous protein evolution through many rounds of mutations, selection...rate Phage infection results in a complete ABE protein. If the phage delivers an active variant of the...

...explore how engineering fields rely on rapid prototyping to develop and test hypotheses. Students then...then use cellular extracts as a means to rapidly prototype DNA circuits and logic gates. This BioBuilder-...projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub teams’ biological ...

...In 2017, Lenny Teytelman, CEO of protocols.io, organized a panel to discuss reproducibility issues in...2018 and involved presentations and a handout on protocol sharing, material sharing, computational reproducibility...management plans, electronic lab notebooks, wet lab protocols, reagent sharing, bioinformatic tools, data and...

...target pathogenic proteins, as well as in the lab to deplete expression of proteins of interest. RNAi ..., I. X., Grunseich, C., Fox, J., et al., Human proteins that interact with RNA/DNA hybrids. Genome Res...

...neurons and depending on the choice of channel protein, they can trigger neuronal excitation or inhibition... using recombinant adeno-associated virus. Curr Protoc Microbiol. (2013); Chapter 14:Unit14D.5. PubMed...Learn about Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...specific to the type of module it is (Pol 2 protomer, Cas protein, ect...). Whatever individual components...approach, it’s easy to switch out reporters, Cas proteins, guide cassettes, promoters, N’ and/or C’ terminus...

...the Type IIS restriction enzyme and thermocycler protocol. It will also identify overhangs with a high potential...possible, NEB also optimized the thermocycler protocols and made sure that T4 ligase was the correct choice...screening for selection, failed under normal cycling protocols, and required a 48-hour incubation at 37 °C. While...

...other plasmids in the deposit, NCBI sequence and protein data, as well as available backbone sequences. ...type  Any tags, selectable markers, and fusion proteins listed for each plasmid  If the plasmids pass...Educational Resources Sequence Analysis of a Plasmid Protocol ...

... for packaging, however, the isoforms of these proteins, as well as the viral LTRs differ. Therefore, ...posts for updates on the most recent deposits, protocols, and tips for using retroviral vectors in your...of your choice. More information and a detailed protocol on how to use the pLKO.1 vector can be found here...

...programs can be put in place to provide community protection. Toehold switches for pathogen detection In ...reported the development of a rapid design and prototyping pipeline that can take pathogen sequence information...critical needs and to further develop our rapid prototyping and sensor assembly pipeline for future emerging...

...off-target editing than Cas9, confirming that this protein is well suited for genome editing.  Find Cpf1 Plasmids...100 nt.) However, the newly described non-CRISPR protein NgAgo is smaller than any of these options (2.7...eSpCas9 and SpCas9-HF1. Both AsCpf1 and LbCpf1 ribonucleoproteins (RNPs) failed to induce off-target editing...