We narrowed to 740 results for: NSI

...categories: probes and editors. A probe array may consist of at least two sensor modules targeting a specific...auxin inducible degron (AID) system has been extensively used in C. elegans since 2015 to conditionally... which can be easily visualized.  The tool-kit consists of highly useful vectors compatible with Gibson.... Check out the new additions.  Image calcium transients in PV interneurons with AAV-S5E2-GCaMP6f, now...

...time moaning about their troubles, Silent or unresponsive clams, who seem to ignore you and your questions...communicate their ideas confidently while also considering the needs of other people. Some students and ...for it. Do not take criticism personally or get defensive. Look at the problem from their viewpoint, and... you even when you meet in person (silent or unresponsive clam), you will probably need support from your...

...which spectral flow cytometry requires special considerations. Conventional flow cytometry During conventional...cytometry for a given experiment, it is important to consider how many parameters you want to use in a given...and spectral flow cytometry, it is important to consider the expression levels of the markers you are including...molecules conjugated together) can be especially sensitive to batch effect. For example, when a PE molecule...

...gotten larger, easier, and more comprehensive thanks to the consistent development and improvement of ... for each experiment. Additional considerations One of the downsides to using AAV is the limited packaging...

...The pegRNA consists of a target-complementary spacer, a scaffold sequence, and an extension sequence containing...screens such as PRIDICT/PRIDICT2.0 provide valuable insights into pegRNA editing efficiencies for specific ...experiments, thus saving you time, sweat, and money on extensive optimization efforts. This holds true for any ...

...most starting materials, and is not particularly sensitive. The fluorescent signal can be affected by AT-...Image courtesy of: Ygonaar    Taqman is more sensitive than Sybr, with less background noise, and is ...amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination of ...approach is right for your experiment. You’ll have to consider:  Resources needed Most molecular biology labs...

...Overall the general strategy for protein expression consists of transfecting cells with your DNA template of... Mammalian systems can express proteins both transiently or through stable cell lines. Both methods produce...recombinant proteins from plants however can be expensive and time consuming as plant tissues themselves...applications. Cell-free expression systems however, are expensive and very technically challenging to use. Alyssa...

...all seriousness, EGFP/GFP has a real nontrivial propensity to noncovalently dimerize. That means that your...to not produce artifacts in your experiments. Considerable effort has gone into making FPs monomeric, yet...actually monomeric, at least in practical terms. The propensity of a particular type of molecule to form a dimer...study of GFP-based FRET, with application to intrinsically unstructured proteins. Protein Sci, 2007. 16...

...RNA-guided gene drives in the summer of 2014 (after extensive discussions with biosecurity experts, ecologists...gene drives intended for eventual release should consider building an appropriate immunizing reversal drive...not use them whenever applicable?Against this, consider the cost of an accidental release. Science relies...trusted to handle such a powerful technology responsibly. An accidental release would be particularly ...

...DDBJ, and PDB sequences and may be the most comprehensive for searching. Timesaving Tip #1: If you know...searches! Now, before you click the BLAST button, consider the Program Selection parameter, as this will ...the alignment at a more distal amino acid. We consistently run our blastx searches with the “Low complexity...101: How to Verify Your Plasmid Plasmids 101: An Inside Look at NGS Plasmid Quality Control Learn about...