Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 834 results for: Eras

Showing: 691 - 720 of 834 results

  2. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ... to pursue their scientific curiosity but can literally put their work on the shoulders of giants. The... and blueprints has the potential not only to accelerate scientific progress, but also to enable science...

  3. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...MOI, often resulting in a drop in transduction coverage compared to just one vector.   Q2: Do you think...a desire for very high MOI on top of very high coverage. Please see this paper for more information about...

  4. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ... of an antibody isotype? An isotype determines several key characteristics of an antibody as well as the...in their heavy chain constant regions result in several unique physical characteristics of these five isotypes...

  5. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...CRISPR gene editing methods relied solely on Cas9. Overall, these approaches have two main drawbacks: 1) ...plasmids; for multiple edits, single plasmids are generally more efficient. Pro-Tip Make sure to reverse the...

  6. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...expressing recombineering proteins Red/ET from a temperature-sensitive plasmid. Homologous recombination then.... Using commercially available high-fidelity polymerases such as KOD and Phusion, which are recommended...

  7. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    Published
    June 2, 2015, 6:06 p.m.
    ...they examined gRNA expression levels and indel generation for two PTG constructs in rice protoplasts. Compared...human cells. Nissim L, Perli SD, Fridkin A, Perez-Pinera P, Lu TK. Mol Cell. 2014 May 22;54(4):698-710. ...

  8. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...auxotrophic selection in E. coli? Additional considerations HIS3 L-hisitidine no S. cerevisiae  no yes...ade6+ purine (adenine)   S. pombe  no no   Considerations when using auxotrophic selection Of course,...

  9. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...  Prime editing improvements By Angela Holmes Several labs have recently shared some exciting plasmid...systems improve gene editing performance at therapeutically relevant gene targets and expand the application...

  10. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...individual colors to 6. A gRNA with all three hairpins generates white light. All 6 gRNAs can be expressed in a...separate vector. Figure 2: CRISPRainbow generates 6 unique colors and a white composite. gRNAs with...

  11. Human Germline Editing Using CRISPR

    Type
    Blog Post
    Published
    Aug. 10, 2017, 2:19 p.m.
    ...human embryos has been met with a storm of media coverage. But the paper published August 2nd in Nature ...present.   Another issue surrounding CRISPR-based therapy is the level of testing needed to define whether...

  13. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...plasmids, such as special growth instructions (temperature, strain, media supplements), suggested sequencing...re like us, you need a strategy for processing several sequencing reactions in parallel. We have a few...

  15. Plasmids 101: Golden Gate Cloning

    Type
    Blog Post
    Published
    Aug. 27, 2015, 2:30 p.m.
    ...and synthetic biology Synthetic biologists have leveraged the power of Golden Gate cloning into a modular...cloning or Gibson assembly. Another important consideration is the design of flanking overhangs. Although...

  17. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    Published
    Aug. 24, 2021, 1:15 p.m.
    ...binding biotin), is conjugated to the enzyme horseradish peroxidase (HRP). Finally, the substrate of HRP... mx+b?). Figure 5: A standard curve is generated by plotting OD against known concentrations of...

  20. Engaging with science and society at pgEd

    Type
    Blog Post
    Published
    Sept. 20, 2018, 12:17 p.m.
    ... of society or devoid of social context. This “general public” audience comprises the taxpayers who fund...be affected by the knowledge and applications generated from this research. At both a practical and ethical...

  21. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...folks at the makerspace comfortable with next-generation sequencing data and the bioinformatics pipeline...resources to pursue it. Since makerspaces are generally funded on a membership model - everyone contributes...

  22. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...purposes, but mainly for the delivery of various therapies and viral vectors. This injection sounds very ... that require ingestion, such as experimental therapies or pain relief, straight to the stomach. This ...

  23. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...other departments/laboratories? How long is the average PhD in this department? How has the department ...research focuses on Drosophila-parasite pathogen interactions.  ...

  24. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type
    Blog Post
    Published
    Feb. 4, 2020, 2:15 p.m.
    ...protein family. What sets them apart is the way they generate their color: they strongly absorb visible light...hydrophilic amino acids to reduce non-specific interactions with cytosolic proteins. This step is important...

  25. Fluorescent Proteins 101: Fluorescent Protein Timers

    Type
    Blog Post
    Published
    May 4, 2017, 2:30 p.m.
    ...timers may be sensitive to changes in oxygen, temperature, and pH, but timer activity can be calibrated...dynamics in a synthetic circuit (Saxena et al.) In a general sense, FP timers can be used in any situation where...

  26. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...Also, avoid unnecessary talking because talking generates microbe-laden aerosols that may enter the hood... CO2 and get agitated with sudden changes in temperature (especially if you have air conditioning on) ...

  27. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ... I needed help with: wouldn’t it be fun to get several of the labs in our program to write short, funny...of content they share and produce and how they interact with their followers. It’s also been a great place...

  28. Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards

    Type
    Blog Post
    Published
    May 22, 2015, 4:58 p.m.
    ...conclusively shown that barrier protocols are vulnerable to human error. And with gene drives, one mistake... M. Concerning RNA-guided gene drives for the alteration of wild populations. eLife e03401 (2014). doi...

  29. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...process has changed with the introduction of next generation sequencing!  Choosing a BLAST program Of the...amount of time to perform the search as well as the overall alignment results. The default setting is “Optimize...

  30. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type
    Blog Post
    Published
    Aug. 20, 2020, 2:30 p.m.
    ...7 mutations found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition...engineering was Cas12a (formerly Cpf1), a nuclease that generates double strand breaks in target genes resulting...
Showing: 691 - 720 of 834 results