We narrowed to 750 results for: Tro;

1. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...genome, and showed success in models of muscular dystrophy. In this case, Konermann et al. targeted MAPT,...

2. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ...Because the performance of a fluorescent protein strongly depends on both the biological system as well ...

3. Luminescent Imaging with Nano-lanterns

   Type

   Blog Post

   Published

   May 25, 2017, 2:30 p.m.

   ...probes, with sufficient signal for in vivo and in vitro imaging. Saito et al. used this Nano-lantern to ...

4. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...They might be researching enhancer elements that control the transcription of a gene a megabase away, or...

5. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

   Type

   Blog Post

   Published

   Nov. 5, 2019, 1:28 p.m.

   ...both small and potentially less likely to elicit a strong immune response, factors that may make it especially...

6. How Addgene Creates Content

   Type

   Blog Post

   Published

   Nov. 17, 2022, 2:15 p.m.

   ...  Susanna Stroik...

7. Plasmids 101: Screens vs. Selections

   Type

   Blog Post

   Published

   Nov. 10, 2022, 2:15 p.m.

   ... Susanna Stroik...

8. How to Keep a Lab Notebook for Bioinformatic Analyses

   Type

   Blog Post

   Published

   June 2, 2016, 2:30 p.m.

   ...difficult to accurately call a file. Version control your commonly used scripts: If you edit a general...

9. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   Published

   July 10, 2014, 7:17 p.m.

   ...equipment that still works. “We use functional spectrometers from the early 1990s that were free and free...

10. Summer SciComm Series: Cognitive Load

    Type

    Blog Post

    Published

    June 16, 2022, 1:15 p.m.

    ... audience.  More resources on the Addgene blog Intro to our Summer SciComm Series...

11. Announcing the Winners of the 2021 Michael Davidson and Roger Tsien Commemorative Conference Awards

    Type

    Blog Post

    Published

    Aug. 5, 2021, 12:15 p.m.

    ...in pMinDisplay (PDGFR anchoring domain), in electroporated in rat hippocampal cultured neurons, imaged...

12. Reproducibility for Everyone: Lessons from an Open Science Collaboration

    Type

    Blog Post

    Published

    Feb. 28, 2019, 1:34 p.m.

    ...practices for reproducibility: data management plans, electronic lab notebooks, wet lab protocols, reagent sharing...

13. Visualizing Protein Turnover In Situ

    Type

    Blog Post

    Published

    Jan. 16, 2018, 3:20 p.m.

    ...protein synthesis and degradation. These processes control the abundance of proteins in cells. Homeostatic...

14. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...create a barcode for your plate, you will get in trouble if you don’t qualify what kind of “plate” you mean...

15. Developing Lab Management Software for Biology

    Type

    Blog Post

    Published

    Aug. 25, 2016, 2:30 p.m.

    ...individually keep track of any failures and do troubleshooting on their own. For a few samples this isn’t ...

16. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type

    Blog Post

    Published

    Dec. 16, 2015, 3:30 p.m.

    ...Created with BioRender.com. To decrease the electropositivity of the HNH/RuvC groove, Slaymaker et al. made...

17. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...similar to an academic research environment” with a strong focus on publishing, but still made it so she could...

18. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...plasmids — more on that in a minute! Fun fact! Electroporation is one popular method scientists use to promote...

19. Making CRISPR Plasmids Using Fragmid

    Type

    Blog Post

    Published

    Aug. 29, 2024, 1:15 p.m.

    ... in the hands of the Doench lab. Additionally, controls available in the system are validated and were...

20. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...techniques that you can use antibodies for in our Intro to Antibodies post Resources on Addgene.org Browse...

21. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    ...allow it. Before you start your presentation, introduce yourself to your audience. Pay attention to...

22. Engaging with science and society at pgEd

    Type

    Blog Post

    Published

    Sept. 20, 2018, 12:17 p.m.

    ...communication posts Extracurricular activities for a strong science career path Resources on Addgene.org ...

23. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

    Type

    Blog Post

    Published

    June 26, 2018, 1:33 p.m.

    ... Decades ago, other labs discovered that the in vitro functions of 11 different Arg-mutant enzymes could...

24. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...boiling point of water. Dr. Terns gave a great introduction to the repeated and distinct aspects of the ...

25. Illuminating Epigenetics with A FRET Based Biosensor

    Type

    Blog Post

    Published

    Nov. 19, 2015, 3:30 p.m.

    ...living cells where methylation of these sites was controlled, the reporter consistently showed significantly...

26. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ...mEPSPs has been possible with conventional electrophysiological methods, it has remained difficult with ...

27. Viral Vectors 101: Producing Your rAAV

    Type

    Blog Post

    Published

    July 16, 2024, 1:15 p.m.

    ...cell-associated (Vandenberghe et al., 2010). For certain in vitro and in vivo applications, you can consider using...

28. Transferable Skills: Negotiation

    Type

    Blog Post

    Published

    March 3, 2022, 2 p.m.

    ...purchases do we prioritize in a lab with both electrophysiologists and molecular biologists? What is their...

29. Polymerase Chain Reaction Overview and Applications

    Type

    Blog Post

    Published

    Aug. 10, 2021, 1:15 p.m.

    ...a polymerase chain reaction (PCR). PCR is an in vitro method in which a small amount of DNA can be copied...

30. Hot Plasmids and Viral Preps - May 2021

    Type

    Blog Post

    Published

    May 18, 2021, 1:15 p.m.

    ...CRISPR/Cas9 constructs. zCas9i is a high efficiency intron-optimized SpCas9-coding gene. SlugCas9 is a compact...