We narrowed to 557 results for: ESP

1. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   Published

   Sept. 7, 2017, 12:17 p.m.

   ... then handled. Additionally, many cell lines, especially the endocrine cell lines we most commonly work...

2. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   Blog Post

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   June 22, 2017, 2:30 p.m.

   ...commonly FPs with red and green wavelengths, respectively, are selected (6). These previous blog posts...

3. Fluorescent Proteins 101: Fluorescent Protein Timers

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   Blog Post

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   May 4, 2017, 2:30 p.m.

   ...monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed mK-GO somewhat...

4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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   Blog Post

   Published

   Dec. 10, 2020, 1 p.m.

   ... Mouse Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy 26:432–442 . https://doi.org...

5. Viral Vectors 101: Producing Your rAAV

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   Blog Post

   Published

   July 16, 2024, 1:15 p.m.

   ... https://doi.org/10.1089/hum.2010.107 Zen, Z., Espinoza, Y., Bleu, T., Sommer, J. M., & Wright, J. F. ...

6. Twenty Years of Addgene Sharing: CRISPR

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   Blog Post

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   April 18, 2024, 1:15 p.m.

   ... effectors, made available in 2013 and 2016, respectively, quickly became popular, and have maintained...

7. Degrading DNA with Cascade-Cas3

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   Blog Post

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   Feb. 11, 2025, 2:15 p.m.

   ...The nuclease domain of Cas3 is considered weak, especially compared to the power of Cas9. However, by continually...

8. Common Injection Routes in Mice

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   Blog Post

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   Jan. 22, 2025, 9:42 p.m.

   ...intradermal injections is studying inflammatory responses, as this method is useful for analyzing localized...

9. Plasmids 101: Degron Tags

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   Blog Post

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   March 14, 2024, 1:15 p.m.

   ...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...

10. Antibodies 101: Conventional vs Spectral Flow Cytometry

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    Blog Post

    Published

    Aug. 3, 2023, 1:15 p.m.

    ...fluorescent molecules conjugated together) can be especially sensitive to batch effect. For example, when ...

11. Transferable Skills Guide: Managing a Team

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    Blog Post

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    Jan. 30, 2018, 2:42 p.m.

    .... They will make mistakes and you will take responsibility (within reason!).  Try to buffer them from ...

12. Plasmids 101: Optimizing Plasmid Yields

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    Blog Post

    Published

    May 26, 2016, 2:30 p.m.

    ... shaken around 220 RPM, but smaller cultures, especially those in deep-well plates, require a faster shaking...

13. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    Blog Post

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    April 19, 2016, 2:30 p.m.

    ...Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles...

14. Experimenting with New Careers while in Grad School

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    Blog Post

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    Feb. 25, 2016, 3:30 p.m.

    ...excellent way to meet people with similar interests! Especially as someone interested in doing science outreach...

15. PITChing MMEJ as an Alternative Route for Gene Editing

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    Blog Post

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    Feb. 23, 2016, 3:30 p.m.

    ...proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh is a robust...

16. Light Sheet Fluorescence Microscopy

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    Blog Post

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    July 16, 2015, 2:30 p.m.

    ...limitation of LSFMs using single side illumination, especially with large tissue, is that any obstacles (e.g...

17. Plasmids 101: Multicistronic Vectors

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    Blog Post

    Published

    Sept. 9, 2014, 8:20 p.m.

    ...Detecting cells that are expressing your gene, especially if you are studying a novel gene, is not always...

18. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    Published

    June 12, 2014, 3:30 p.m.

    ... people to use the Golden Gate cloning system especially with plants in mind. When it’s ready, I’ll make...

19. SciComm: Taking Science to Elementary and Middle Schools

    Type

    Blog Post

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    July 9, 2019, 12:52 p.m.

    ...As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...

20. Simplify Cloning with in vivo Assembly

    Type

    Blog Post

    Published

    Oct. 18, 2018, 12:37 p.m.

    ...vector with restriction enzymes and adding the corresponding homologous sequences to the insert by PCR. ...