We narrowed to 554 results for: ESP
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TypeBlog PostPublishedMarch 14, 2024, 1:15 p.m....protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...
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Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....The nuclease domain of Cas3 is considered weak, especially compared to the power of Cas9. However, by continually... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....intradermal injections is studying inflammatory responses, as this method is useful for analyzing localized... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m..... They will make mistakes and you will take responsibility (within reason!). Try to buffer them from ... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....fluorescent molecules conjugated together) can be especially sensitive to batch effect. For example, when ... -
Starter guide to induced pluripotent stem cells (iPSCs) part 1: A renaissance in regenerative medicine
TypeBlog PostPublishedOct. 30, 2018, 1:04 p.m....fibroblasts via retroviral and lentiviral infections respectively [9, 10]. These stellar works led to a Nobel ... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m....CRISPR protein tagging does have its limitations, especially when trying to perform high-throughput experiments... -
Cloning Mammalian Cells with the Agarose Method
TypeBlog PostPublishedSept. 7, 2017, 12:17 p.m.... then handled. Additionally, many cell lines, especially the endocrine cell lines we most commonly work... -
Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles
TypeBlog PostPublishedJune 22, 2017, 2:30 p.m....commonly FPs with red and green wavelengths, respectively, are selected (6). These previous blog posts... -
Fluorescent Proteins 101: Fluorescent Protein Timers
TypeBlog PostPublishedMay 4, 2017, 2:30 p.m....monomeric fluorescent proteins mKO and mCherry, respectively. While Tsuboi et al developed mK-GO somewhat... -
Plasmids 101: Optimizing Plasmid Yields
TypeBlog PostPublishedMay 26, 2016, 2:30 p.m.... shaken around 220 RPM, but smaller cultures, especially those in deep-well plates, require a faster shaking... -
When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells
TypeBlog PostPublishedApril 19, 2016, 2:30 p.m....Snapp. Stop using EGFP/GFP for fusion proteins! Despite multiple studies in high profile journal articles... -
Experimenting with New Careers while in Grad School
TypeBlog PostPublishedFeb. 25, 2016, 3:30 p.m....excellent way to meet people with similar interests! Especially as someone interested in doing science outreach... -
PITChing MMEJ as an Alternative Route for Gene Editing
TypeBlog PostPublishedFeb. 23, 2016, 3:30 p.m....proper insertion at the 5’ and 3’ junctions, respectively. These results indicate that PITCh is a robust... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....limitation of LSFMs using single side illumination, especially with large tissue, is that any obstacles (e.g... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....Detecting cells that are expressing your gene, especially if you are studying a novel gene, is not always... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m.... people to use the Golden Gate cloning system especially with plants in mind. When it’s ready, I’ll make... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit... -
Capturing a Moment with Marc Zimmer’s “The State of Science”
TypeBlog PostPublishedJuly 30, 2020, 1:15 p.m....colleagues who read it with me, the consistent response from scientist and non-scientist alike was that... -
SciComm: Taking Science to Elementary and Middle Schools
TypeBlog PostPublishedJuly 9, 2019, 12:52 p.m....As a person with many roles and responsibilities, I have to think a lot about how to balance those roles...