Skip to main content
Addgene
Showing: 461 - 480 of 545 results

  1. Twenty Years of Addgene Sharing: CRISPR

    Type
    Blog Post
    Published
    April 18, 2024, 1:15 p.m.
    ... effectors, made available in 2013 and 2016, respectively, quickly became popular, and have maintained...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...protein for imaging. Given its popularity and widespread usage, a proteolysis-targeting chimera (PROTAC...

  6. Plasmids 101: Optimizing Plasmid Yields

    Type
    Blog Post
    Published
    May 26, 2016, 2:30 p.m.
    ... shaken around 220 RPM, but smaller cultures, especially those in deep-well plates, require a faster shaking...

  10. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...limitation of LSFMs using single side illumination, especially with large tissue, is that any obstacles (e.g...

  11. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...Detecting cells that are expressing your gene, especially if you are studying a novel gene, is not always...

  15. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ....1371/journal.pone.0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification...

  16. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ...expression and detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining...

  17. CRISPR 101: RNA Editing with Cas13

    Type
    Blog Post
    Published
    July 31, 2020, 12:30 p.m.
    ...enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly edit...

  19. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...vector with restriction enzymes and adding the corresponding homologous sequences to the insert by PCR. ...
Showing: 461 - 480 of 545 results