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We narrowed to 836 results for: Eras

Showing: 461 - 480 of 836 results

  1. The Developmental Studies Hybridoma Bank: Over 25 Years of Antibody Sharing

    Type
    Blog Post
    Published
    March 10, 2015, 2:25 p.m.
    ...ways of generating antibodies, for example through the use of less toxic adjuvants; 2) generating complex... The minimal fees from distribution funds our operation. Antibodies are glycoproteins that are part of...increased avidity to high profile antigens; and 4) generating mAbs against new cancer biomarkers. We believe...

  2. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...) (Miller et al., 2005). Although this tight interaction is not covalent, later work showed that introducing...ER and mitochondria reveals dynamic organelle interactions. a) Labeling scheme. b) COS-7 cell ER labeled...without any cloning at all! As mentioned above, several variants of these tags have been developed over...https://doi.org/10.1021/cb900062k Gautier, A., Juillerat, A., Heinis, C., Corrêa, I. R., Kindermann, M.... Pick, H., Vogel, H., & Johnsson, K. (2003). A general method for the covalent labeling of fusion proteins...

  3. Michael Koeris' Journey from Grad Student to Entrepreneur: The Story of Sample 6

    Type
    Blog Post
    Published
    Jan. 21, 2016, 4:52 p.m.
    ...not right for the development of phage-based therapeutics. After a brief stint at the venture capital ...Novophage's focus. The company went from developing therapeutics to developing phage-based tools for detecting...develop tests for food products as opposed to therapeutics 15:15 - 16:26 : Means for testing for pathogens...for Dr. Koeris to transition from developing therapeutics to developing a diagnostic? 36:53 - 38:00 : ...

  4. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    Published
    Dec. 30, 2015, 3:30 p.m.
    ...are required to ensure that you get full library coverage in pooled library screens. Availability and Use...metalloproteinase-9 secretion, and identifying lysine methyltransferase substrates. We’re incredibly happy that lentiCRISPR...of the many plasmids being distributed to and accelerating research in labs all around the world. We thank...Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR...

  5. Inntags: Innovative Protein Epitope Tagging

    Type
    Blog Post
    Published
    Nov. 10, 2015, 3:30 p.m.
    ...of different conformations and thus potentially interact with and alter the proteins they label. In fact...chaperone Ydj1, both of which display sophisticated interactions with multiple other proteins. Again, little ...indicating that they compromised protein-protein interactions. If you’re looking to a tag a protein, inntags...tags of choice for examining protein-protein interactions, particularly in single-molecule or high-throughput...

  6. Your Top Requested Plasmid in 2016!

    Type
    Blog Post
    Published
    Jan. 11, 2017, 8:47 p.m.
    ... the key component of most CRISPR systems and generates double strange breaks at target sites 5' of the...your 20 nucleotide guide sequence of choice (for generation of a gRNA that will target SpCas9 to the approrpriate... expression post transfection by western blot. Overall, this is a versatile mammalian expression vector...Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some Common Ways to Deliver CRISPR...

  7. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    Published
    June 17, 2015, 9:04 p.m.
    ...DayCon. DayCon is a one-day conference aimed at the general public that provides an accessible introduction...conference-style experience that was accessible to the general public both in terms of cost and content level....of disease, cancer, and viral infections. An interactive lunch with student presenters and board members... of shorter, more narrowly focused lectures, interactive panels, and a student poster session. All DayCon...

  8. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    Published
    Jan. 23, 2014, 3:23 p.m.
    ...available at Addgene, also gives the option to stack several expression cassettes onto a single T-DNA in subsequent...rounds, dramatically reducing the time required to generate multi-construct transgenic plants. In the most...choice, BsaI, which can be very sensitive to temperature fluctuations. He says their lab learned the hard...into the public domain in the process. “We had several motivations for making GreenGate available through...

  9. Which Fluorescence Microscopy Technique is Best for Me?

    Type
    Blog Post
    Published
    Oct. 10, 2017, 1:57 p.m.
    ...towards the objective is collected and focused onto a camera for visualization. Illuminating a sample in the...is excited at one time and captured in a single camera exposure. This is much faster than relying on raster...fast, sensitive detector such as a scientific CMOS camera. If plasma membrane dynamics are the main focus... excitation occurs through the fluorophore’s interaction with an evanescent energy field. Lightsheet microscopes... to a higher photon flux via the more efficient camera detector and lack of a light-reducing pinhole. ...

  10. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ...s how science progresses after all, with each generation of scientists standing on the shoulders of their...Technology Policy issued a memorandum calling on Federal agencies with over $100 million in annual R&D expenditures...support increased public access to the results of federally-funded research. 2)      In June, the White House...Bohannon published an article describing a sting operation in which he submitted a phony paper to a list ...

  11. IDP and your PI: A Roadmap for Career Planning and Personal Development

    Type
    Blog Post
    Published
    July 21, 2015, 2:30 p.m.
    ...then formulate a plan to further develop your transferable skills and reach your career goals.      Although...subject matter discussed at the meeting, but the overall tone of the meeting is almost more important. As...Wunderlich Z, Estrada J, Park J, Bragdon MD, Lopez Rivera F, Biette KM, DePace AH. Mol Cell. 2015 Jun 4;58... What Makes a Good Manager Anyway Developing Transferable Skills During Science Training ...

  12. Inside Addgene's 10 Year Anniversary Gala

    Type
    Blog Post
    Published
    July 3, 2014, 3:48 p.m.
    ... the interactive photo graffiti wall. I had a lot of fun playing the prototype of an interactive game ...about her grandfather’s humble beginnings as an operator of a printing press. Just as her grandfather used...the newspaper, Addgene uses E. coli bacteria to generate copies of plasmids. The community of scientists...

  13. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...opportunities, protocols, publications, and more to accelerate the pace of research into COVID-19. While this...opportunities, and community response initiatives. Accelerate the translational impact of research Universities...important for creating research synergies and accelerating the translational impact of research activities...Alliance at the CCRP2. Other groups have focused on iterating on methods for coronavirus research, sharing resources...

  14. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen receptor ligand binding...about new recombinases suitable for this system. Gerald Rubin’s lab sought to make complex genome modifications...specific recombination site for each enzyme, were generated to test recombination efficiency. When expressed...PubMed. Reduction of Cre recombinase toxicity in proliferating Drosophila cells by estrogen-dependent activity...

  15. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...and Dr. Paul Stoffels from J&J. The panel was moderated by a non-scientist from Bloomberg news. Given ...who shared first authorship on their seminal, cooperative paper in Science.  Interestingly, this project...finding they have been able to use CRISPR/Cas9 to generate mice with genes knocked out in the insulin signaling..., open academic exploration in this area will accelerate the launch of clinical applications. During ...

  16. London Calling - Addgene’s New Europe Office in London

    Type
    Blog Post
    Published
    June 10, 2014, 3:20 p.m.
    ... brand new Europe office located in London and operated in collaboration with LGC Standards. And now you... VII. During my years in research, I developed several new plasmids, including: plasmids to study the ...and shared by others to develop an anti-tumour therapy using antibodies targeting dendritic cells. These...sharing those ideas with others. This love of interacting with people and talking about science makes her...

  17. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

    Type
    Blog Post
    Published
    June 11, 2020, 1:34 p.m.
    ...behaviors. Scientists continue to engineer opsins to operate at increasingly faster kinetics. The opsin ChETA...body. Administering the drug once can result in several hours of neuronal excitation or inhibition. This...sustained activation period is beneficial for use in therapeutic or behavioral applications where drawn out effects... by Designer Drugs): Chemogenetic Tools with Therapeutic Utility. Annual Review of Pharmacology and Toxicology...

  18. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...of time, especially at room temperature or at 4°C. Storing DNA: temperature and longevity You should consider...molecular biology like restriction enzymes and polymerases. In the situation where you need to use plasmid...Plasmid DNA can be stable at 4°C or even room temperature for a short period, and there are indications...

  19. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...lights (Baier et al., 2009).  Zebrafish behaviors Several behavioral assays can be done on zebrafish at 5...neurons that trigger the CPG (central pattern generator) (Wyart et al., 2009). The CPG is a neural circuit...circuit involved in locomotion that generates periodic motor commands for rhythmic movements like spontaneous...

  20. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...blot and immunofluorescence. GST  Glutathione S-Transferase (GST) is a 26 kDa tag which can be isolated with...Purification of His fusion proteins from E. coli is generally more straightforward and of a higher purity than...washing. Nonetheless, this tag can be purified with several different resin types and is a fairly small tag...and other qualities of each tag class, they are generally best suited for their primary application. Ready...
Showing: 461 - 480 of 836 results