We narrowed to 566 results for: FIE

1. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

   Type

   Blog Post

   Published

   April 6, 2021, 12:30 p.m.

   ...our instrument and begin the analysis. We use a modified version of the Python program described by Joung...

2. Mouse Modeling, Part 2: Breeding and Crossing Mice

   Type

   Blog Post

   Published

   Aug. 6, 2019, 12:55 p.m.

   ...with the goal is to generate mice that have two modified genes in their genome, and assuming Mendalian ...

3. Design Tips for Prime Editing

   Type

   Blog Post

   Published

   Jan. 23, 2025, 2:15 p.m.

   ...schematic. Created with BioRender.com. The pegRNA specifies both the genomic location to be edited and the...

4. Lab Automation at Addgene

   Type

   Blog Post

   Published

   March 22, 2018, 1:28 p.m.

   ...through the mail due to laws concerning genetically modified organisms. Since Addgene is very proud to help...

5. Adeno Associated Virus (AAV) for Cell and Gene Therapy

   Type

   Blog Post

   Published

   Nov. 7, 2017, 1:59 p.m.

   ...al. "Off-target-free gene delivery by affinity-purified receptor-targeted viral vectors." Nature communications...

6. Viral Vectors 101: Virus Safety

   Type

   Blog Post

   Published

   June 22, 2023, 1:15 p.m.

   .... (AAVS1)). For these reasons, AAV is usually classified as a BSL-1 safety level. Adenovirus is similar...

7. Tips for Technical Support Calls

   Type

   Blog Post

   Published

   Sept. 20, 2016, 2:39 p.m.

   ... And finally, provide feedback. If you are unsatisfied with the nature or speed of a response, let your...

8. Cpf1 Update: Comparison to Cas9 and NgAgo

   Type

   Blog Post

   Published

   July 14, 2016, 2:30 p.m.

   ...nucleases and NgAgo is an important next step for the field. Based on what we know now, Cpf1 may be the best...

9. Plasmids 101: Modular Cloning

   Type

   Blog Post

   Published

   March 12, 2024, 1:15 p.m.

   ...multiple steps in which a precursor molecule is made, modified (sometimes many times), and isolated afterwards...

10. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...of Yellow Fluorescent Protein (YFP). The team identified a new YFP variant, mGold. mGold was found to ...

11. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...Plasmid Reference Pages Just Starting Work in a New Field? Check out Our Plasmid Collections! Join the Addgene...

12. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type

    Blog Post

    Published

    Jan. 21, 2015, 1:30 p.m.

    ...Bramson, author “Coping with Difficult People,” identified seven types of difficult people: Hostile-aggressives...

13. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type

    Blog Post

    Published

    June 5, 2014, 3:32 p.m.

    ... indicate their presence. They are genetically modified organisms, and GMOs have a bad reputation. The...

14. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type

    Blog Post

    Published

    June 18, 2020, 1:15 p.m.

    ...treatment or prophylaxis of COVID-19. Researchers identified nanobodies that bind to coronavirus spike proteins...

15. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ...early 2000s, a handful of chromoproteins were identified in sea anemone (Chan et al., 2006). Why do these...

16. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ...have additional questions. If you or your lab identifies a potential collaborator from the meeting, reach...

17. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type

    Blog Post

    Published

    Feb. 7, 2019, 2:16 p.m.

    ... guide and the DNA target. CFD scores are then modified (“transformed”) such that weak interactions (e.g...

18. Hot Plasmids: Summer 2025

    Type

    Blog Post

    Published

    Aug. 28, 2025, 1:15 p.m.

    ...fibronectin intrabody generated with mRNA display. Modified from Rivera & Huang, et al. (2025) under CC BY-NC-ND...

19. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ...measured pixel and the soma. The initiation site identified in panel (C) is indicated; red line is exponential...

20. Viral Vectors 101: Voltage Indicators

    Type

    Blog Post

    Published

    Jan. 30, 2024, 2:15 p.m.

    ...containing GEVIs. Chemigenetic sensors are better classified by their fluorescent reporter and the mechanism...