We narrowed to 553 results for: PPL
-
TypeBlog PostPublishedMay 24, 2018, 1:20 p.m....Proceedings of the National Academy of Sciences 100.suppl 1 (2003): 11861-11865. PubMed PMID: 12917490. PubMed...
-
Pathways Over Time Plasmids Engage Students in Functional Genomics Research
TypeBlog PostPublishedMay 22, 2018, 1:38 p.m....ura3 mutants and cannot grow without uracil supplementation (6). Expression of MET/Met genes from the pYES2.1... -
Science communication: 9 Strategies to Get Your Foot in the Door
TypeBlog PostPublishedSept. 27, 2017, 1:23 p.m....of competitive TED talk – to win the audience’s applause with your performance. When your institute opens... -
Fluorescent Proteins 101: Introduction to FRET
TypeBlog PostPublishedJune 27, 2017, 1:03 p.m...., which could monitor physical stresses or even apply forces (Gayrard and Borghi 2016) to a selected protein... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....descriptive purpose focuses on the practical application of the plasmid and will help a scientist outside... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....excitation or inhibition upon light (or drug) application. Genetic manipulation can also reduce expression... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....regions with Vivid (Vvd) photodimers. Upon the application of blue light, Vvd dimerizes bringing the C-terminal... -
Hot Plasmids - October 2020
TypeBlog PostPublishedOct. 13, 2020, 1:15 p.m....Hybrid for Multiplexed Editing and screening Applications (CHyMErA) Knockout Libraries express hybrid ... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m....B, Bondy-Denomy J (2020) Anti-CRISPR protein applications: natural brakes for CRISPR-Cas technologies.... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....its design changes for every new prime editing application. And optimizing its sequence is important, as... -
Making CRISPR Plasmids Using Fragmid
TypeBlog PostPublishedAug. 29, 2024, 1:15 p.m....validated and were chosen because of their broad applicability in different model systems. What’s the error... -
CRISPR in the Clinic
TypeBlog PostPublishedNov. 2, 2023, 1:15 p.m....disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to disease correction in adult stem cells... -
High Complexity Golden Gate Assembly with NEB’s DAD
TypeBlog PostPublishedAug. 1, 2023, 1:15 p.m....Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https... -
Viral Vectors 101: Chemogenetics
TypeBlog PostPublishedJuly 11, 2023, 1:15 p.m....chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org... -
Viral Vectors 101: Virus Safety
TypeBlog PostPublishedJune 22, 2023, 1:15 p.m....protection are key to lab safety and the same principles apply to viral handling. Gloves are always required and... -
Antibodies 101: Stripping and Reprobing Western Blots
TypeBlog PostPublishedAug. 1, 2024, 1:15 p.m....retaining residual antibodies. If you have already applied antibodies and need to dry the membrane for storage... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....time. Antibodies made from animals are limited in supply and producing antibodies from plasmids is much ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....from Tang et al., 2020. G-baToN can also be applied to other fluorophore/antibody pairs. Because the... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....trials support the utility of AAV as a broadly applicable gene delivery tool, aspects of AAV biology should... -
Tips for Technical Support Calls
TypeBlog PostPublishedSept. 20, 2016, 2:39 p.m...., I’m confident that these tips and tricks will apply to any biology-related customer support interaction...