We narrowed to 482 results for: SEC

...and has been suggested to cause cytotoxicity [5]. Second, instead of using a donor-plasmid, you could use...

...through Addgene’s viral service and the methods sections of scientific articles (gasp). To my delight, ...

... in your system. It is very rare for a methods section to include all of the variables that need to be...

...active in vitro as well as in vivo in yeast, plant, insect, and mammalian cells, including human cells. piggyBac...

... was interested in microbes until the end of my second year! Conclusion and other pieces of advice Give...

...also happen at the state and local level. The big secret about science policy that I didn’t learn until ...

...PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control...

...from Pyrococcus furiosus." Acta Crystallographica Section F: Structural Biology and Crystallization Communications...

...fluorescent proteins to make better reporters and the second couples NLuc to fluorescent proteins to make better...

...fact is that we are citizens first and scientists second and that it is our duty to tell our science stories...

...Labeling scheme. b) COS-7 cell ER labeled with Halo-Sec61β with SiR-CA (magenta) and outer mitochondrial membrane...

...of CRISPR overall. Type I systems degrade large sections of DNA thanks to the dual action power of Cas3...

... flow cytometry. Let's briefly go through each section of this article again. In the beginning, we talked...

...can transfer energy directly via resonance to the second fluorophore (the acceptor) if the two probes are...

...kit.   Do you want eco-friendly options? It’s no secret that labs produce a lot of waste. If you are looking...

.... ABE Adenine Base Editor Converts A → G; the second type of base editor invented. CGBE C → G Base...

...first gRNA using restriction enzyme BbsI and the second gRNA using restriction enzyme BsaI. If you’re working...

...Higher efficiency ✓ ✓ ✓ ✓ ✓   ✓ Second sgRNA to guide nicking of the unedited strand ...

...vectors are better suited for these situations. The second scenario requires higher levels of safety and as...

...design is not as efficient in genome editing as the second design (in pX330/PX335), so we recommend people...