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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 590 results for: abo.1

Showing: 461 - 480 of 590 results

  3. Another Pathway into Cells: iTOP

    Type
    Blog Post
    Published
    June 23, 2015, 8:37 p.m.
    ...vesicles. Okada CY, Rechsteiner M. Cell. 1982 May;29(1):33-41. PubMed. Protein transduction: unrestricted... a protocol has been established) may be less laborious than viral purification, and it does not raise...

  4. Four Factors that Differentiate the Stem Cell Field

    Type
    Blog Post
    Published
    Sept. 28, 2016, 2:35 p.m.
    ...group of researchers special: 1. Stem cell scientists care deeply about ethics Ethics must play a prominent...the Addgene Blog Learn about Efforts to Fight Rare disease with iPSCs Read About Addgene's Outreach Efforts...the first clinical trial with iPSCs with his collaborator Takahashi in 2014 – just 8 years after the OSKM...Researcher Darrell Kotton 2. Stem cell scientists care about patients As any grad student who has spent years... a big picture perspective: Dr. Yamanaka talked about his clinical trial patients with advanced macular...proof that these researchers are thriving in a collaborative, rather than a combative, field. Of course they... in the Microbiology Community Sign Up to Write About Stem Cells Resources on the Addgene Wesbite Find...

  5. Visualizing Translation at the Single Molecule Level

    Type
    Blog Post
    Published
    Aug. 1, 2017, 1:15 p.m.
    ... cell types and physiological states. References 1. Ingolia, Nicholas T., et al. “Genome-wide analysis...Additional Resources the Addgene Blog Learn More about SunTag Catch up on Your GFP Background Choose the...

  6. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...Networking 101-Building Relationships for Success”. Choose 1-2 tactics to try. One of my favorites is making the...work if both parties are diligent about replying and thinking about good questions and answers. Professional...Sometimes, it is not who you know but who knows about you. I found some other great tips for finding mentors...Medical School researcher Connie Cepko to learn about her mentoring style. Mentors at home and away We...Catalyst Report “Making Mentoring Work” to learn about what makes a good mentoring program. If your organization...much less threatening! If they say no, don’t worry about it. It is a compliment to ask someone to share their...

  7. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...to do that than in the Addgene blog?  Fig. 1: One of our favorite content collections is our Help...We also want to tell you about things you didn’t know you wanted to know about, like research breakthroughs... want to know more about! You can also suggest blog topics or even reach out about writing a guest post...technical skills, and experiences from thousands of laboratories that makes up our Help Center, blog, website...

  8. 5 Great Apps for Lab Life

    Type
    Blog Post
    Published
    Sept. 22, 2016, 2:30 p.m.
    ...instead conveniently provide them on your smart phone. 1. Lab Archives by LabArchives The single, most important...saved and cannot be deleted, so there is no worry about misplacing your results or losing that lab notebook...enzyme, or name to discover all the information about that restriction enzyme or share it via email with... this app. However, if you get quizzed randomly about a hazardous chemical and can’t find your lab’s MSDS... new researchers or students beginning to learn about biochemistry. The least this app will do is allow...

  9. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...and also by a lactose analogue, IPTG (isopropyl β-D-1-thiogalactopyranoside). (To be completely accurate...antibiotic selection here at Plasmids 101, we can talk about an even more specific method of screening your cloning... plasmid is a great start when cloning, but how about being able to choose those that contain plasmid ... a widely used method to do just that! (To read about other screening strategies, check out our screening... called α-complementation. The system described above was put to practical use in the following way. Scientists...

  10. Scientists Map the SARS-CoV-2-Human Interaction Network

    Type
    Blog Post
    Published
    April 9, 2020, 1:15 p.m.
    ...faster. Years of science, done in weeks Figure 1: Sending out 80 FedEx envelopes in a single day --...following up on these leads as we speak, with collaborators in Paris and New York. We want YOU to join the...impact on virus replication. While we and our collaborators are starting these experiments, there's so much...are particularly interested in facilitating collaborative science, and making it accessible to all people...

  11. Components of CRISPR/Cas9

    Type
    Blog Post
    Published
    Feb. 2, 2016, 5 p.m.
    ...individual protein has all the components necessary to: 1. Bind to a guide RNA The guide RNA, which will be ...that you know about all the different components of CRISPR, It's time to start thinking about how you can...Use the following flow chart to get you thinking about the types of experiments you're planning on doing...

  12. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...present in the data such as synapsin, hsyn synapsin-1, human synapsin, hSynap. Matching We capture multiple...this multidisciplinary effort and cross-team collaboration (thanks to all Addgenies involved). The new ...Additional resources from the Addgene blog Learn about other features on Addgene's website Find a step-...to using Addgene's new search Read to learn more about why we redesigned our homepage and mascot ...

  13. The CRISPR Software Matchmaker: A New Tool for Choosing the Best CRISPR Software for Your Needs

    Type
    Blog Post
    Published
    Nov. 3, 2015, 3:30 p.m.
    ...first of only three such databases, EENdb (published 1 Jan 2013, no longer available), is a simple catalogue...follow him on Twitter @CMacPhD.          References  1. Ghorbal, Mehdi, et al. "Genome editing in the human...databases mentioned above or, even better, first-hand knowledge from laboratories employing CRISPR on ... CRISPR system was involved. Today, that same laboratory enjoys a successful edit rate of over 90% in ...targetable NGG PAM sites whereas the human genome has about 300 million. With such a sparsely targetable genome...worse by an ever increasing marketplace. Since 2013 about 11 tools have been added every year.   Breaking ...databases are still young and will require a collaborative effort from the community in order to succeed...

  14. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...-fDIO mCherry 83900 AAVrg pAAV-mDlx-GFP-Fishell-1 Biosensors Plasmid Serotype Name 83899 AAVrg...vector name and serotype you’d like to be notified about. Chemogenetics Plasmid Serotype Name 44362 ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  15. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    Published
    Jan. 21, 2025, 2:15 p.m.
    ...pitfalls? Here's what you need to know.   Figure 1: Management can seem a mystery at first! Image used...clarifying roles, I eased the tension and improved collaboration. The good news: It’s a journey, not a final...of postdocs focusing on molecular biology and metabolism. He now guides fellow PhDs in their careers with...

  16. Plasmids 101: Methylation and Restriction Enzymes

    Type
    Blog Post
    Published
    June 30, 2016, 2:30 p.m.
    ...into account, the digest pattern depicted in Lane 1 would be expected for a XbaI/NotI digest. The program...questions is the same--methylation! Read on to learn about how DNA methylation may affect your restriction ...be methylated. Using the same example plasmid as above (which has the XbaI site blocked by methylation)...

  17. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ...the guides are relatively unstable.   Figure 1: CRISPRainbow labeling. Two MS2, PP7, or boxB sequences...the Addgene blog Download our CRISPR eBook Learn about fluorescent protein-based tools Read all CRISPR ...

  18. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    Published
    March 14, 2019, 1:54 p.m.
    ...vector-exosomes), exosome AAVs, or exo-AAVs (Figure 1). The researchers discovered that these particles ...vectors 101 eBook Resources on Addgene.org Learn about Addgene's viral service Find viral protocols and...

  19. Using CRISPR/Cas9 to Edit Disease Out of the Genome

    Type
    Blog Post
    Published
    June 23, 2022, 1:15 p.m.
    ...through the use of nanoparticles. [6].    References 1. Zhang B. 2021. “CRISPR/Cas gene therapy.” Journal...therapeutic.” There are many fundamental questions about CRISPR/Cas biology still left to answer as well,...

  20. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...as an endogenous RNA imaging tool.   Fig. 1: Csm, shRNA, and Cas13 were compared head-to-head ... minutes of FUS.  Similar constructs to the one above can be made for expressing your favorite T-cell ...
Showing: 461 - 480 of 590 results