We narrowed to 609 results for: cas

1. Understanding GPCR Signaling Using TRUPATH

   Type

   Blog Post

   Published

   Dec. 14, 2020, 2:15 p.m.

   ...in the initiation of a vast number of signaling cascades, inducing different cellular responses. One of...

2. Viral Vectors 101: Parts of the AAV Packaging Plasmid

   Type

   Blog Post

   Published

   Dec. 3, 2020, 2:15 p.m.

   ... domains, all four Rep proteins share a common helicase and ATPase domains. To function, the Rep proteins...

3. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

   Type

   Blog Post

   Published

   June 11, 2020, 1:34 p.m.

   ... experiment. Viral delivery is the same in both cases, but activation depends on small molecule activation...

4. What's the Best Way to Elute and Store Your Plasmid DNA?

   Type

   Blog Post

   Published

   June 4, 2020, 1:22 p.m.

   ... your plasmid at -20°C or even at -80°C, in this case your preparation can be eluted in water or in your...

5. Fostering communication in remote collaborations

   Type

   Blog Post

   Published

   April 21, 2020, 1:14 p.m.

   ...addressed and new ideas generated. Furthermore, this casual communication reflects the type of informal communication...

6. Management for Scientists: Giving Feedback

   Type

   Blog Post

   Published

   Feb. 6, 2015, 2:46 p.m.

   ...appropriately to get the message across. Listen to our podcast interview with Harvard Medical School researcher...

7. 10 Ways to Share Your Science!

   Type

   Blog Post

   Published

   May 13, 2014, 8:34 p.m.

   ... story and science involves characters – in this case you – on a quest to ask and answer questions and...

8. Advice for Choosing a Research Project

   Type

   Blog Post

   Published

   April 17, 2014, 2:45 p.m.

   ...research project. What will the impact be in a best case scenario? If you get your dream results, what will...

9. Tetbow: Bright Multicolor Labeling for Neuronal Tracing

   Type

   Blog Post

   Published

   Jan. 24, 2019, 2:24 p.m.

   ...transgene. When multiple copies of the transgene cassette are introduced, stochasticity will result in a...

10. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type

    Blog Post

    Published

    Dec. 13, 2018, 1:14 p.m.

    ...others, such as AAV2, low yields are the norm. In the case of AAV2 this is thought to be due to the viral particle...

11. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    Published

    Aug. 18, 2023, 4:15 p.m.

    ...mRNA, directly translated into proteins, or, in the case of retroviruses, reverse transcribe their gRNA into...

12. What the HEK?

    Type

    Blog Post

    Published

    April 18, 2023, 1:15 p.m.

    ...produce recombinant proteins at high levels. In a case where clinical applications of recombinant proteins...

13. Plasmids 101: TOPO Cloning

    Type

    Blog Post

    Published

    Oct. 27, 2016, 2:30 p.m.

    ...topoisomerase site inserted into a beta-galactosidase cassette allowing a researcher to perform blue-white screening...

14. Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life

    Type

    Blog Post

    Published

    April 12, 2018, 1:02 p.m.

    ...classroom Engineering the Plant Genome Using CRISPR/Cas9 pSiM24: Simplifying Plant Genetic Engineering Resources...

15. Identifying Sequence Elements with SnapGene's Feature Database

    Type

    Blog Post

    Published

    Dec. 21, 2017, 2:06 p.m.

    ... crafted a detection algorithm that tolerates occasional mismatches or indels. Empirical tests indicated...

16. The Strength of Story Telling

    Type

    Blog Post

    Published

    Dec. 19, 2017, 2 p.m.

    ...your structure. Develop your protagonist In most cases, the protagonist will be you. Make sure to highlight...

17. Science Careers: Unruly Interests Feed Many Paths

    Type

    Blog Post

    Published

    Sept. 26, 2017, 12:12 p.m.

    ...’s scientific discoveries into an entertaining podcast? Look at our multimedia producer and online editor...

18. The Golden Stopwatch Award: Recognizing Facilitators of Research

    Type

    Blog Post

    Published

    Sept. 5, 2019, 1:06 p.m.

    ...deposit agreements to basically instantaneous in many cases.  Suggest that they get an Addgene account for Tech...

19. Antibodies 101: Epitope Availability

    Type

    Blog Post

    Published

    Jan. 11, 2022, 2 p.m.

    ...specific configurations or conditions. In those cases, pay special attention to designing your controls...

20. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ...single colonies and testing multiple preps. In some cases, we suggest gel extraction to isolate the full, ...